Invitrogen

Claudin 1 Polyclonal Antibody (MH25)

Name: Claudin 1 Polyclonal Antibody (MH25)
Brand: Invitrogen
SKU: 71-7800
Price: 5124.00 CNY
Availability: InStock

高级验证

88个已发表图片

194 篇参考文献

查看其他26个Claudin 1抗体

说明书

Certificates

实验方案

问题与答案

技术支持

说明书

Certificates

实验方案

问题与答案

技术支持

说明书

Certificates

实验方案

问题与答案

技术支持

Additional Information:

Claudin 1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

图: 1 / 94

Claudin 1 Antibody (71-7800) in IHC (P)

Immunohistochemistry analysis of Claudin-1 showing staining in the membrane and weak cytoplasm staining of paraffin-embedded human liver tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for ... View More

Claudin 1 Antibody in Immunohistochemistry (IHC)

Claudin 1 Antibody in Western Blot, Immunohistochemistry (WB, IHC)

Claudin 1 Antibody in Immunocytochemistry (ICC/IF)

Claudin 1 Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)

Claudin 1 Antibody in Western Blot, Immunohistochemistry (Frozen) (WB, IHC (F))

Claudin 1 Antibody in Immunohistochemistry (Frozen) (IHC (F))

Claudin 1 Antibody in Western Blot, Immunoprecipitation (WB, IP)

Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

Product Image Gallery

Immunogen image

Immunogen Image Claudin 1 Polyclonal Antibody (MH25)

产品信息

71-7800

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1-2 µg/mL

查看80篇已发表文章 80篇已发表文章

免疫组化 (IHC)

查看46篇已发表文章 46篇已发表文章

免疫组化（石蜡） (IHC (P))

1:10-1:100

查看8篇已发表文章 8篇已发表文章

免疫组化（冰冻） (IHC (F))

查看5篇已发表文章 5篇已发表文章

免疫细胞化学 (ICC/IF)

查看46篇已发表文章 46篇已发表文章

酶联免疫吸附实验 (ELISA)

0.1-1.0 µg/mL

免疫沉淀 (IP)

查看4篇已发表文章 4篇已发表文章

其他PubMed (Misc)

查看5篇已发表文章 5篇已发表文章

产品规格

种属反应

Dog, Human, Mouse, Rat

已发表种属

Avian, Bovine, Chicken, Dog, Fish, Human, Mouse, Pig, Rabbit, Rat, Tag, Virus

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

克隆号

MH25

抗原

A synthetic peptide derived from the C-terminus of the human/mouse Claudin-1 protein.

3D表位/免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.25 mg/mL

100 µg

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7.4

内含物

0.1% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_2533997

产品详细信息

This antibody reacts with the ~ 22 kDa Claudin-1 protein. Reactivity was confirmed by western blotting and immunofluorescence. Positive controls include MDCK cells (dog), Caco-2cells (human), mouse liver, rat liver, and rat kidney.

NOTE: This antibody strongly cross reacts with the Claudin-3 protein. It has been observed in western blotting inhibition studies using Caco-2 cell lysates, that this antibody is inhibited by addition of Claudin-3 peptide. For a Claudin-1 specific antibody that does not cross-react with Claudin-3, please order Cat. No. 519000 (Rb x Claudin-1) instead.

靶标信息

Claudin-1 is a member of the transmembrane protein family claudins located in cell-cell tight junctions and it acts as a co-receptor for HCV entry into hepatic cells. Claudins are abundant in luminal epithelial sheets where they maintain epithelial cell polarity. Claudin-1 is expressed in most tissues such as bladder, fallopian tube, liver, pancreas, prostate, and skin.

仅用于科研。不用于诊断过程。未经明确授权不得转售。