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Invitrogen
Claudin 3 Polyclonal Antibody
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FIGURE: 1 / 93
Claudin 3 Antibody (34-1700) in ICC/IF
Immunofluorescence analysis of Claudin-3 was performed using 70% confluent log phase NIH:OVCAR-3 cells. The cells were fixed with 4% paraformaldehyde for 5 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with Claudin 3 Polyclonal Antibody (Product # 34-1700) at 1:100 dilution in 0.1% BSA, incubated at 4 degree ... View More
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.jpg?time=20240507?time=20240507 "Claudin 3 Antibody (34-1700) in IHC")
.jpg?time=20240507?time=20240507 "Claudin 3 Antibody (34-1700) in WB, IHC")
.jpg?time=20240507?time=20240507 "Claudin 3 Antibody (34-1700) in WB, IHC")
Product Details
34-1700
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Dog,
Human,
Mouse
Published species
Amphibian,
Bovine,
C. elegans,
Dog,
Fish,
Goat,
Hamster,
Human,
Mouse,
Pig,
Plant,
Rabbit,
Rat,
Rhesus monkey,
Sheep,
Virus,
Xenopus,
Zebrafish
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide derived from the C-terminal region of mouse Claudin-3
Conjugate
Form
Liquid
Concentration
0.25 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4
Contains
0.1% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody is specific for the 22 kDa Claudin-3 protein. Reactivity has been confirmed with mouse liver and lung homogenates, canine MDCK and human MCF-7 cell lysates by western blotting, and with formalin-fixed, paraffin-embedded (FFPE) human normal colon and colon cancer tissues by immunohistochemistry. For best results in immunohistochemistry with formalin-fixed, paraffin-embedded (FFPE) tissues, heat induced epitope retrieval (HIER) with citrate buffer, pH 6.0, is required prior to staining.
Target Information
Claudin 1-8 proteins are a family of transmembrane proteins associated with tight junctions. Tight junctions are specialized regions of cell to cell contact; made up of network of strands to act as a molecular gasket for preventing the leakage of ions, water etc. between cells. They are abundant in luminal epithelial sheets where they maintain epithelial cell polarity. Different tissues exhibit different Claudin composition. Differential expression of this gene has been observed in different types of malignancies, including breast cancer, ovarian cancer, hepatocellular carcinomas, urinary tumors, prostate cancer, lung cancer, head and neck cancers, thyroid carcinomas, etc. Alternatively spliced transcript variants encoding different isoforms have been found.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Claudin-3; Clostridium perfringens enterotoxin receptor 2; CPE-R 2; CPE-receptor 2; hRVP1; integral membrane protein claudin-3; Rat ventral prostate.1 protein homolog; ventral prostate.1 protein homolog; ventral prostate.1-like protein
Gene Aliases: AI182374; C7orf1; CLDN3; CPE-R2; CPETR2; HRVP1; mRVP1; RVP1
UniProt ID: (Human) O15551, (Dog) Q95KM5, (Mouse) Q9Z0G9
Entrez Gene ID: (Human) 1365, (Dog) 403648, (Mouse) 12739
response to hypoxia
epithelial cell morphogenesis
signal transduction
calcium-independent cell-cell adhesion via plasma membrane cell-adhesion molecules
protein homooligomerization
protein heterooligomerization
bicellular tight junction assembly
positive regulation of protein phosphorylation
negative regulation of cell proliferation
positive regulation of gene expression
negative regulation of gene expression
establishment of endothelial blood-brain barrier
regulation of cell morphogenesis
positive regulation of cell migration
negative regulation of cell migration
actin cytoskeleton reorganization
cell junction maintenance
negative regulation of wound healing
positive regulation of wound healing
regulation of membrane permeability
positive regulation of bicellular tight junction assembly
positive regulation of metallopeptidase activity
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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