Rockland

Collagen Type IV Polyclonal Antibody, Biotin

Name: Collagen Type IV Polyclonal Antibody, Biotin
Brand: Rockland
SKU: 600-406-106

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说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (1)

Collagen Type IV Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Collagen Type IV Antibody (600-406-106) in IHC (P)

Immunohistochemistry of Rabbit Anti-Collagen IV Biotin Conjugated Antibody. Tissue: human kidney (Left) with strong red staining observed in glomeruli and liver (Right) with strong staining in sinusoids. Fixation: formalin fixed paraffin embedded. Antigen retrieval: steamed in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes. Primary antibody: Collagen IV antibody at 10 µg/mL for 1 h at RT. ... View More

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Immunogen image

Immunogen Image Collagen Type IV Polyclonal Antibody, Biotin

产品信息

600-406-106

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:2,000-1:10,000

免疫组化（石蜡） (IHC (P))

1:1,000-1:5,000

酶联免疫吸附实验 (ELISA)

1:20,000-1:100,000

免疫沉淀 (IP)

1:100

产品规格

种属反应

Bovine, Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Anti-Collagen Type IV was produced by repeated immunizations with Collagen Type IV from human and bovine placenta.

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偶联物

Biotin Biotin Biotin

形式

Lyophilized

浓度

1 mg/mL

纯化类型

Affinity chromatography

保存液

0.02M potassium phosphate, pH 7.2, with 10mg/mL BSA, 0.15M NaCl

内含物

0.01% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

Store vial at 4° C prior to restoration. Restore with 0.1 mL of deionized water (or equivalent). For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of restoration.

Typically negligible cross-reactivity against other types of collagens was detected by ELISA against purified standards. Some class-specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type IV collagens and has negligible cross-reactivity with Type I, II, III, V and VI collagens. Non-specific cross-reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.

靶标信息

Collagen IV is a type of collagen protein that is a major component of the basement membrane in various tissues, including the kidneys, lungs, and skin. It forms a network-like structure that provides support and stability to the surrounding tissue. Collagen IV is composed of three alpha chains, which are encoded by the COL4A1, COL4A2, and COL4A3 genes. Mutations in these genes can lead to various genetic disorders, such as Alport syndrome and Goodpasture syndrome, which affect the kidneys and lungs, respectively.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: arresten; COL4A1 NC1 domain; Collagen 4; Collagen alpha-1(IV) chain; Collagen alpha-2(IV) chain; Collagen alpha-3(IV) chain; Collagen alpha-4(IV) chain; Collagen alpha-5(IV) chain; Collagen alpha-6(IV) chain; collagen IV, alpha-1 polypeptide; collagen IV, alpha-3 polypeptide; Collagen IV, alpha-4 polypeptide; collagen IV, alpha-5 polypeptide; collagen IV, alpha-6 polypeptide; collagen of basement membrane, alpha-1 chain; collagen of basement membrane, alpha-4 chain; collagen of basement membrane, alpha-5 chain; collagen of basement membrane, alpha-6; collagen, type IV, alpha 1; collagen, type IV, alpha 3 (Goodpasture antigen); collagen, type IV, alpha 4; collagen, type IV, alpha 5; collagen, type IV, alpha 6; dA149D17.3; dA24A23.1; dJ889N15.4 (Collagen Alpha 6(IV)); Goodpasture antigen; tumstatin; Type IV Collagen

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基因别名: ASLN; ATS; BSVD; CA44; CA54; COL4A1; COL4A2; COL4A3; COL4A4; COL4A5; COL4A6; CXDELq22.3; DELXq22.3; DFNX6; ICH; POREN2; RATOR