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Description: The monoclonal antibody X53 recognizes human, mouse, porcine, guinea pig, and rat Collagen Type X. X53 does not cross-react with collagen types I, II, II, IV, or VI. Collagen Type X is a homotrimeric short chain collagen. Collagen Type X has limited expression in normal adult tissues, primarily being expressed during bone growth and development. Expression is localized to the peri- and extracellular matrix of hypertrophic chondrocytes during endochondral ossification and also plays a role in cartilage mineralization. Collagen Type X is elevated in many tumor types with expression being localized in the vasculature. Mutations to the gene COL10A1, which encodes human Collagen Type X, is associated with Schmid type metaphyseal chondrodysplasia.
Applications Reported: This X53 antibody has been reported for use in immunohistochemical staining of frozen tissue sections, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, microscopy, and immunocytochemistry.
Applications Tested: This X53 antibody has been tested by immunohistochemistry on formalin-fixed paraffin embedded human tissue using low pH antigen retrieval and can be used at less than or equal to 5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Filter Recommendation: When using this eFluor® 570 antibody conjugate, we recommend a filter that will capture the 570 emission wavelength (for example, Excitation 545/25, 565LP, Emission 605/70). A standard Alexa Fluor® 555 or TRITC filter is acceptable.
Excitation: 555 nm; Emission: 570 nm
Type X collagen is a product of hypertrophic chondrocytes and has been localized to presumptive mineralization zones of hyaline cartilage.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
蛋白别名: Collagen alpha-1(X) chain; collagen X, alpha-1 polypeptide; collagen, type X, alpha 1; collagen, type X, alpha 1(Schmid metaphyseal chondrodysplasia); pro alpha 1; procollagen, type X, alpha 1; Schmid metaphyseal chondrodysplasia; type X collagen; type X collagen, alpha 1
基因别名: Col10; Col10a-1; COL10A1
UniProt ID: (Human) Q03692, (Mouse) Q05306
Entrez Gene ID: (Pig) 448809, (Human) 1300, (Dog) 100856711, (Mouse) 12813, (Rat) 25681, (Guinea pig) 106026924