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Invitrogen
Connexin 30 Polyclonal Antibody (Z-PP9)
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Connexin 30 Antibody (71-2200) in ICC/IF
Immunofluorescence analysis of Connexin-30 was done on 70% confluent log phase Caco-2 cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with Connexin-30 Rabbit polyclonal Antibody (Product # 71-2200) at 2 µg/mL in 1% BSA and incubated for 3 hours at room te... View More
产品信息
71-2200
应用
建议稀释比
已发表文章
产品规格
种属反应
Cat,
Human,
Mouse,
Rat
已发表种属
C. elegans,
Cat,
Chicken,
Human,
Mouse,
Non-human primate,
Rat,
Xenopus
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
克隆号
Z-PP9
抗原
A synthetic peptide derived from the unique C-terminus of the mouse Connexin 30 protein
偶联物
形式
Liquid
浓度
0.25 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7.4
内含物
0.1% sodium azide
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
This antibody is specific for the Connexin 30. Cross-reactivity with the highly related Connexin 26 protein or with other Connexin family members has not been detected. Reactivity of this antibody has been confirmed by western blotting using cell lysates derived from adult rat, mouse, human, and cat brains.
靶标信息
Gap junctions allow the transport of ions and metabolites between the cytoplasm of adjacent cells. They are formed by two hemichannels, made up of six connexin proteins assembled in groups. Each connexin protein has four transmembrane segments, two extracellular loops, a cytoplasmic loop formed between the two inner transmembrane segments, and the N- and C-terminus both being in the cytoplasm. The specificity of the gap junction is determined by which connexin proteins comprise the hemichannel. In the past, connexin protein names were based on their molecular weight, however the new nomenclature uses sequential numbers based on which form of the gap junction is present. This gene encodes one of the connexin proteins. Mutations in this gene have been found in some forms of deafness and in some families with hidrotic ectodermal dysplasia.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: connexin 30; Connexin-30; Cx30; ectodermal dysplasia 2, hidrotic (Clouston syndrome); Gap junction beta-6 protein; gap junction membrane channel protein beta 6; gap junction protein, beta 6 (connexin 30); gap junction protein, beta 6, 30kDa
基因别名: AA958971; CX30; Cxn-30; D14Bwg0506e; DFNA3; DFNA3B; DFNB1B; ECTD2; ED2; EDH; GJB6; HED; HED2
UniProt ID: (Human) O95452, (Mouse) P70689
Entrez Gene ID: (Human) 10804, (Mouse) 14623, (Rat) 84403
