Invitrogen

Cytochrome C Monoclonal Antibody (37BA11)

Name: Cytochrome C Monoclonal Antibody (37BA11)
Brand: Invitrogen
SKU: 45-6100

高级验证

7个已发表图片

17 篇参考文献

查看其他50个Cytochrome C抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

Cytochrome C Antibody in Immunocytochemistry (ICC/IF)

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Cytochrome C Antibody (45-6100) in ICC/IF

Immunofluorescence analysis of Cytochrome C was performed using 70% confluent log phase Hep G2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with Cytochrome C Monoclonal Antibody (37BA11) (Product # 45-6100) at 1 µg/mL in 0.1% BSA, incubated at 4 deg... View More

Cytochrome C Antibody in Western Blot (WB)

Cytochrome C Antibody in Flow Cytometry (Flow)

Cytochrome C Antibody in Immunohistochemistry (IHC)

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产品信息

45-6100

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.25-1 µg/mL

查看9篇已发表文章 9篇已发表文章

免疫组化 (IHC)

查看3篇已发表文章 3篇已发表文章

免疫组化（石蜡） (IHC (P))

查看2篇已发表文章 2篇已发表文章

免疫细胞化学 (ICC/IF)

0.5-2 µg/mL

查看1篇已发表文章 1篇已发表文章

流式细胞分析 (Flow)

1 µg/mL

亲和纯化 (AP)

查看1篇已发表文章 1篇已发表文章

其他PubMed (Misc)

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产品规格

种属反应

Bovine, C. elegans, Human, Mouse, Rat

已发表种属

C. elegans, Human, Mouse, Rat, Yeast

宿主/亚型

Mouse / IgG2a, kappa

分类

Monoclonal

类型

Antibody

克隆号

37BA11

抗原

Bovine heart Cytochrome C

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

IgG fraction

保存液

HEPES buffered saline

内含物

0.02% sodium azide

保存条件

-20°C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

AB_2533821

产品详细信息

45-6100 was used successfully in the western blot analysis of cytochrome C using human breast cancer cell line lysates.

Positive control: Isolated mitochondria from Human, Bovine, Rat and Mouse heart; Human fibroblasts; HeLa cells.

WB: Predicted molecular weight: 14 kDa. ICC/IF: Heat induced antigen retrieval in 0.1 M Tris-HCl, 5% urea, pH 9.5 for 5 min at 95°C improves signal.

靶标信息

Cytochrome C is an electron transporting protein that resides within the intermembrane space of the mitochondria, where it plays a critical role in oxidative phosphorylation and the production of cellular ATP. Release of cytochrome C from the mitochondria to the cytosol is triggered by apoptotic stimuli. In the cytoplasm, the cytochrome C binds apoptotic protease activating factor which activates the apoptotic initiator procaspase 9. Cytosolic cytochrome C functions in the activation of caspase 3, an ICE family molecule that is a key effector of apoptosis.

仅用于科研。不用于诊断过程。未经明确授权不得转售。