Invitrogen

DAB2IP Polyclonal Antibody

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问题与答案

问题与答案

问题与答案

抗体检测数据 (2)

DAB2IP Antibody in Immunocytochemistry (ICC/IF)

DAB2IP Antibody in Immunocytochemistry (ICC/IF)

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DAB2IP Antibody (PA5-144652) in ICC/IF

Immunocytochemistry analysis of DAB2IP in SH-SY5Y cells. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 &(8)451;, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were incubated with DAB2IP polyclonal antibody (Product # PA5-144652) with a dilution of 1:200 in 2% negative goat serum overnight at 4 &(8)451;. Followed by secondary antibody Goat Anti-Rabbit IgG H&L (iFluor™ 488) at a dilution of 1:1,000. PBS instead of the primary ... View More

DAB2IP Antibody in Immunocytochemistry (ICC/IF)

DAB2IP Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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DAB2IP Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry analysis of DAB2IP in SH-SY5Y cells. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 &(8)451;, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were incubated with DAB2IP polyclonal antibody (Product # PA5-144652) with a dilution of 1:200 in 2% negative goat serum overnight at 4 &(8)451;. Followed by secondary antibody Goat Anti-Rabbit IgG H&L (iFluor™ 488) at a dilution of 1:1,000. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

DAB2IP Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of DAB2IP in paraffin-embedded human thyroid carcinoma tissue. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with DAB2IP polyclonal antibody (Product # PA5-144652) with a dilution of 1:800 for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen, and tissues were counterstained with hematoxylin and mounted with DPX.

Immunogen image

DAB2IP Polyclonal Antibody

Immunogen Image DAB2IP Polyclonal Antibody

产品信息

PA5-144652

应用

建议稀释比

已发表文章

免疫组化（石蜡） (IHC (P))

1:800

-

免疫细胞化学 (ICC/IF)

1:200

-

产品规格

种属反应

Human, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant protein within human DAB2IP aa 950-1189/1189.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

2 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.4, with 0.1% BSA, 40% glycerol

内含物

0.05% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

Positive control: Mouse brain tissue lysate, rat brain tissue lysate, Hela cell lysate, A431 cell lysate, human thyroid carcinoma tissue, SH-SY5Y.

Predicted band size: 132 kDa

Subcellular Location: Cell membrane, Cytoplasm.

靶标信息

DAB2IP is a Ras GTPase-activating protein (GAP) that acts as a tumor suppressor. The DAB2IP gene is inactivated by methylation in prostate and breast cancers.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: AIP-1; ASK-interacting protein 1; ASK1-interacting protein 1; DAB2 interaction protein; DAB2-interacting protein; DIP1/2; disabled homolog 2 interacting protein; Disabled homolog 2-interacting protein; DOC-2/DAB-2 interactive protein; DOC-2/DAB2 interactive protein; DOC2/DAB2 interactive protein; nGAP-like protein

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基因别名: AF9Q34; AIP-1; AIP1; DAB2IP; DIP1/2; KIAA1743

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UniProt ID: (Human) Q5VWQ8, (Rat) Q6P730

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Entrez Gene ID: (Human) 153090, (Rat) 192126

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GTPase activator activity death receptor binding protein binding SH3 domain binding kinase binding protein kinase binding mitogen-activated protein kinase kinase binding mitogen-activated protein kinase kinase kinase binding phosphatidylinositol-3-phosphate binding protein complex binding signaling adaptor activity Toll-like receptor 4 binding phosphatidylinositol 3-kinase regulatory subunit binding identical protein binding protein homodimerization activity vascular endothelial growth factor receptor 2 binding phosphatidylinositol 3-kinase binding protein phosphatase 2A binding phosphatidylinositol-4-phosphate binding 14-3-3 protein binding cadherin binding involved in cell-cell adhesion protein N-terminus binding GTPase-activating protein

negative regulation of transcription from RNA polymerase II promoter MAPK cascade activation of MAPKKK activity angiogenesis negative regulation of protein phosphorylation inflammatory response activation of signaling protein activity involved in unfolded protein response cell cycle I-kappaB phosphorylation activation of JUN kinase activity negative regulation of cell proliferation extrinsic apoptotic signaling pathway via death domain receptors negative regulation of endothelial cell migration negative regulation of epithelial cell migration negative regulation of epithelial to mesenchymal transition positive regulation of neuron projection development negative regulation of phosphatidylinositol 3-kinase signaling negative regulation of angiogenesis cell motility involved in cerebral cortex radial glia guided migration layer formation in cerebral cortex negative regulation of vascular endothelial growth factor receptor signaling pathway negative regulation of NF-kappaB transcription factor activity negative regulation of toll-like receptor 4 signaling pathway negative regulation of GTPase activity tube formation negative regulation of catenin import into nucleus cellular response to vascular endothelial growth factor stimulus vascular endothelial growth factor receptor-2 signaling pathway reelin-mediated signaling pathway regulation of growth negative regulation of epidermal growth factor receptor signaling pathway positive regulation of apoptotic process regulation of GTPase activity regulation of I-kappaB kinase/NF-kappaB signaling negative regulation of I-kappaB kinase/NF-kappaB signaling regulation of protein complex assembly negative regulation of MAP kinase activity positive regulation of MAPK cascade regulation of protein heterodimerization activity positive regulation of JUN kinase activity positive regulation of GTPase activity negative regulation of phosphatidylinositol 3-kinase activity cellular protein catabolic process innate immune response positive regulation of protein catabolic process negative regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter positive regulation of JNK cascade negative regulation of Ras protein signal transduction negative regulation of fibroblast proliferation neuron projection morphogenesis negative regulation of epithelial cell proliferation intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress negative regulation of G0 to G1 transition negative regulation of ERK1 and ERK2 cascade positive regulation of cell cycle arrest cellular response to lipopolysaccharide cellular response to interleukin-1 cellular response to tumor necrosis factor cellular response to epidermal growth factor stimulus negative regulation of protein serine/threonine kinase activity positive regulation of protein serine/threonine kinase activity endothelial cell apoptotic process negative regulation of canonical Wnt signaling pathway positive regulation of synapse maturation cell-cell adhesion positive regulation of dendrite development regulation of p38MAPK cascade negative regulation of vascular endothelial growth factor signaling pathway positive regulation of proteasomal protein catabolic process positive regulation of IRE1-mediated unfolded protein response negative regulation of cyclin catabolic process positive regulation of neuron migration positive regulation of apoptotic signaling pathway apoptotic process response to unfolded protein negative regulation of cell growth positive regulation of epithelial cell proliferation