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Invitrogen
DAXX Polyclonal Antibody
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FIGURE: 1 / 13
DAXX Antibody (PA5-79137) in ICC/IF
Immunocytochemistry analysis of Daxx using anti-Daxx antibody (Product # PA5-79137) . Daxx was detected in a section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and then incubated with 2μg/mL rabbit anti-Daxx antibody (Product # PA5-79137) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visua... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-79137
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived human Daxx recombinant protein (Position: K56-R345).
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Lyophilized
Concentration
500 µg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5mg BSA
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human K562 whole cell, rat PC-12 whole cell, mouse thymus tissue. IHC: rat intestine tissue, human intestinal cancer tissue. ICC/IF: U20S cell, Hela cell, SMMC-7721 cell, A549 cell. Flow: 293T cell.
Target Information
This gene encodes a multifunctional protein that resides in multiple locations in the nucleus and in the cytoplasm. It interacts with a wide variety of proteins, such as apoptosis antigen Fas, centromere protein C, and transcription factor erythroblastosis virus E26 oncogene homolog 1. In the nucleus, the encoded protein functions as a potent transcription repressor that binds to sumoylated transcription factors. Its repression can be relieved by the sequestration of this protein into promyelocytic leukemia nuclear bodies or nucleoli. This protein also associates with centromeres in G2 phase. In the cytoplasm, the encoded protein may function to regulate apoptosis. The subcellular localization and function of this protein are modulated by post-translational modifications, including sumoylation, phosphorylation and polyubiquitination. Alternative splicing results in multiple transcript variants.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: CENP-C binding protein; DADB-159G18.9; DAXX; death domain-binding protein; HDaxx; death-associated protein 6; death-domain associated protein; ETS1-associated protein 1; Fas death domain-associated protein; Fas-binding protein; MGC126245; MGC126246; SMIM40; unnamed protein product
Gene Aliases: BING2; DAP6; EAP1
UniProt ID: (Human) Q9UER7, (Mouse) O35613
Entrez Gene ID: (Human) 1616, (Rat) 140926, (Mouse) 13163
p53 binding
transcription coactivator activity
transcription corepressor activity
protein binding
enzyme binding
protein kinase binding
protein kinase activator activity
heat shock protein binding
ubiquitin protein ligase binding
histone binding
protein homodimerization activity
androgen receptor binding
RNA polymerase II sequence-specific DNA binding transcription factor binding
transcription regulator inhibitor activity
ATP-dependent H3-H4 histone complex chaperone activity
molecular condensate scaffold activity
JUN kinase binding
kinesin binding
nucleosome assembly
chromatin remodeling
regulation of transcription, DNA-templated
JNK cascade
extrinsic apoptotic signaling pathway via death domain receptors
regulation of gene expression
negative regulation of gene expression
androgen receptor signaling pathway
regulation of protein ubiquitination
telomeric heterochromatin assembly
cellular response to heat
cellular response to unfolded protein
neuron intrinsic apoptotic signaling pathway in response to oxidative stress
regulation of apoptotic process
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
cellular response to cadmium ion
cellular response to copper ion
cellular response to diamide
DNA replication-dependent chromatin disassembly
cellular response to sodium arsenite
negative regulation of transcription from RNA polymerase II promoter
mitotic cytokinesis
negative regulation of protein kinase activity
cell proliferation
response to metal ion
negative regulation of myotube differentiation
PML body organization
regulation of multicellular organism growth
positive regulation of apoptotic process
negative regulation of apoptotic process
positive regulation of protein kinase activity
positive regulation of transcription from RNA polymerase II promoter
cellular response to hydrogen peroxide
protein localization to chromatin
cellular response to interferon-gamma
cellular response to tumor necrosis factor
apoptotic signaling pathway
negative regulation of cellular response to hypoxia
positive regulation of apoptotic signaling pathway
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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