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Invitrogen
DNA-PK Recombinant Rabbit Monoclonal Antibody (SC57-08)
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DNA-PK Antibody (MA5-32192) in ICC/IF
Immunofluorescence analysis of DNA-PK was performed using HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with DNA-PK Rabbit Monoclonal Antibody (Product # MA5-32192) at 1:100 dilution in 0.1% BSA and incubated overnight at 4 degree and then labeled with Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32731) at a dilution of 1:200... View More
产品信息
MA5-32192
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
已发表种属
Human
宿主/亚型
Rabbit
/ IgG
Expression System
HEK293 cells
分类
Recombinant Monoclonal
类型
Antibody
克隆号
SC57-08
抗原
Synthetic peptide within Human DNA PKcs aa 4062-4109
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein A
保存液
TBS, pH 7.4, with 40% Glycerol, 0.05% BSA
内含物
0.05% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
靶标信息
DNA-PK is a heterotrimeric, DNA-dependent protein kinase that initiates signaling cascades in response to DNA damage. DNA-PK is an atypical kinase, closely related to the PI3 kinases, and is often potently inhibited by small molecules designed to target PI3 kinases. DNA-PK encodes a component of the mediator complex, a transcriptional coactivator complex thought to be required for the expression of almost all genes. The mediator complex is recruited by transcriptional activators or nuclear receptors to induce gene expression, possibly by interacting with RNA polymerase II and promoting the formation of a transcriptional pre-initiation complex. Multiple transcript variants encoding different isoforms have been found for DNA-PK.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: DNA-dependent protein kinase catalytic subunit; DNA-PK catalytic subunit; DNPK1; doxorubicin nephropathy; hyper-radiosensitivity of murine scid mutation, complementing 1; p460; severe combined immunodeficiency
基因别名: AI326420; AU019811; DNA-PKcs; DNAPDcs; DNAPK; DNPK1; DOXNPH; dxnph; HYRC; HYRC1; IMD26; p350; p460; PRKDC; scid; slip; XRCC7
UniProt ID: (Human) P78527, (Mouse) P97313
Entrez Gene ID: (Human) 5591, (Mouse) 19090, (Rat) 360748
double-stranded DNA binding
protein kinase activity
protein serine/threonine kinase activity
DNA-dependent protein kinase activity
protein binding
ATP binding
transcription factor binding
enzyme binding
poly(A) RNA binding
nucleotide binding
DNA binding
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
phosphotransferase activity, alcohol group as acceptor
molecular_function
non-receptor serine/threonine protein kinase
protein modifying enzyme
somitogenesis
negative regulation of protein phosphorylation
B cell lineage commitment
pro-B cell differentiation
T cell lineage commitment
negative regulation of immunoglobulin production
positive regulation of immune system process
double-strand break repair
double-strand break repair via nonhomologous end joining
cellular protein modification process
brain development
heart development
cell proliferation
intrinsic apoptotic signaling pathway in response to DNA damage
response to gamma radiation
response to activity
telomere capping
peptidyl-serine phosphorylation
protein destabilization
positive regulation of type I interferon production
cellular response to insulin stimulus
T cell differentiation in thymus
immunoglobulin V(D)J recombination
T cell receptor V(D)J recombination
ectopic germ cell programmed cell death
regulation of circadian rhythm
positive regulation of apoptotic process
negative regulation of apoptotic process
positive regulation of transcription from RNA polymerase II promoter
positive regulation of fibroblast proliferation
rhythmic process
spleen development
thymus development
positive regulation of developmental growth
regulation of smooth muscle cell proliferation
signal transduction involved in mitotic G1 DNA damage checkpoint
double-strand break repair via alternative nonhomologous end joining
negative regulation of cellular senescence
negative regulation of response to gamma radiation
telomere maintenance
double-strand break repair via homologous recombination
immunoglobulin production
DNA repair
protein phosphorylation
cellular response to DNA damage stimulus
response to ionizing radiation
phosphorylation
lymphocyte differentiation
V(D)J recombination
