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Invitrogen
DYKDDDDK Tag Monoclonal Antibody (L5), Alexa Fluor™ 488
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DYKDDDDK Tag Antibody (MA1-142-A488) in WB
Western blot was performed using Anti-DYKDDDDK Tag Monoclonal Antibody (L5), Alexa Fluor 488 (Product # MA1-142-A488) by loading whole cell extracts of untransfected and transiently transfected HEK-293E lysates: untransfected, 40 µg (Lane 1), empty vector control, 40 µg (Lane 2), HA-H3-DYKDDDDK, 40 µg (Lane 3), DYKDDDDK-p65-HA, 40 µg (Lane 4), DYKDDDDK-p65-HA, 20 µg (Lane 5), DYKDDDDK-p65-HA, 10 µg (Lane 6), DYKDDDDK-p65-HA, 5 µg (Lane 7), Myc-p65-V5, 40 µg (Lane 8) and 25 ng of Positope (Lane 9) were electrophoresed using NuPAGE™ 4-12% Bi... View More
产品信息
MA1-142-A488
应用
建议稀释比
已发表文章
产品规格
种属反应
Tag
已发表种属
C. elegans,
Tag
宿主/亚型
Rat
/ IgG2a
分类
Monoclonal
类型
Antibody
克隆号
L5
抗原
DYKDDDDK-tagged mouse Langerin
偶联物
激发/发射光谱
499/520 nm
查看光谱
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein G
保存液
PBS with 1mg/mL BSA
内含物
0.05% sodium azide
保存条件
4°C, do not freeze
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
similar epitope as the FLAG tag from Sigma
靶标信息
DYKDDDDK tag, also known as FLAG tag, is a short peptide sequence that is commonly used as a protein tag to facilitate protein purification, detection, and localization. The tag consists of eight amino acids: Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys. It is often fused to the N- or C-terminus of a protein of interest, allowing for easy identification and isolation of the protein using anti-FLAG antibodies. The DYKDDDDK tag is widely used in molecular biology and biochemistry research, and has been employed in various applications, such as protein-protein interaction studies, protein expression analysis, and protein localization studies. The FLAG tag structure has been optimized for compatibility with the proteins it is attached to, in that it is more hydrophilic than other common epitope tags and therefore less likely to denature or inactivate proteins to which it is appended. FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
