Search
Invitrogen
Dynamin 2 Polyclonal Antibody
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
Additional Information:
{{banner.description}}
FIGURE: 1 / 9
Dynamin 2 Antibody (PA1-661) in ICC/IF
Immunofluorescence analysis of Dynamin 2 was performed using 70% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with Dynamin 2 Polyclonal Antibody (Product # PA1-661) at 1:100 in 0.1% BSA, incubated at 4 degree celsius over... View More
Product Details
PA1-661
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human,
Mouse,
Non-human primate,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic Peptide: C S(760) P T P Q R R P V S S I H P P G R P P A(779)
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein G
Storage buffer
PBS with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
PA1-661 detects dynamin II (Dyn2) from rat, mouse, and human tissues and cells.
PA1-661 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~100 kDa protein representing Dyn2 from HeLa cell lysate.
PA1-661 immunizing peptide corresponds to amino acid residues 760-779 from human Dyn2. This sequence is 95% conserved in mouse and rat Dyn2. PA1-661 immunizing peptide (Cat. # PEP-056) is available for use in neutralization and control experiments.
Target Information
Dynamins represent one of the subfamilies of GTP-binding proteins. These proteins share considerable sequence similarity over the N-terminal portion of the molecule, which contains the GTPase domain. Dynamins are associated with microtubules. They have been implicated in cell processes such as endocytosis and cell motility, and in alterations of the membrane that accompany certain activities such as bone resorption by osteoclasts. Dynamins bind many proteins that bind actin and other cytoskeletal proteins. Dynamins can also self-assemble, a process that stimulates GTPase activity. Five alternatively spliced transcripts encoding different proteins have been described. Additional alternatively spliced transcripts may exist, but their full-length nature has not been determined.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Dynamin 2; Dynamin II; Dynamin UDNM; Dynamin-2; unnamed protein product
Gene Aliases: b2b2159Clo; CMT2M; CMTDI1; CMTDIB; DI-CMTB; DNM2; DYIIAAB; DYN2; DYNII; LCCS5; Udnm
UniProt ID: (Human) P50570, (Rat) P39052, (Mouse) P39054
Entrez Gene ID: (Human) 1785, (Rat) 25751, (Mouse) 13430
nucleotide binding
GTPase activity
protein binding
GTP binding
phosphatidylinositol-4,5-bisphosphate binding
microtubule binding
hydrolase activity
SH3 domain binding
enzyme binding
protein kinase binding
D2 dopamine receptor binding
protein complex binding
phosphatidylinositol 3-kinase regulatory subunit binding
WW domain binding
nitric-oxide synthase binding
membrane traffic protein
G2/M transition of mitotic cell cycle
regulation of transcription, DNA-templated
post-Golgi vesicle-mediated transport
endocytosis
receptor-mediated endocytosis
phagocytosis
autophagy
centrosome cycle
signal transduction
synaptic vesicle budding from presynaptic endocytic zone membrane
antigen processing and presentation of exogenous peptide antigen via MHC class II
regulation of axon extension
receptor internalization
transferrin transport
positive regulation of apoptotic process
stress fiber assembly
positive regulation of transcription, DNA-templated
synaptic vesicle transport
neuron projection morphogenesis
protein polymerization
membrane organization
actin filament bundle organization
membrane tubulation
vesicle scission
negative regulation of membrane tubulation
G-protein coupled receptor internalization
Golgi to plasma membrane transport
spermatogenesis
metabolic process
response to light stimulus
positive regulation of lamellipodium assembly
negative regulation of transforming growth factor beta receptor signaling pathway
regulation of Rac protein signal transduction
response to cocaine
macropinocytosis
positive regulation of nitric oxide biosynthetic process
positive regulation of endocytosis
positive regulation of phagocytosis
cilium morphogenesis
cellular response to carbon monoxide
cellular response to X-ray
cellular response to nitric oxide
positive regulation of substrate adhesion-dependent cell spreading
cellular response to dopamine
regulation of Golgi organization
positive regulation of sodium:potassium-exchanging ATPase activity
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support