Invitrogen

Dynamin 2 Polyclonal Antibody

Name: Dynamin 2 Polyclonal Antibody
Brand: Invitrogen
SKU: PA1-661

6个已发表图片

20 篇参考文献

查看其他14个Dynamin 2抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (3)
已发表图片 (6)

Dynamin 2 Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 9

Dynamin 2 Antibody (PA1-661) in ICC/IF

Immunofluorescence analysis of Dynamin 2 was performed using 70% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with Dynamin 2 Polyclonal Antibody (Product # PA1-661) at 1:100 in 0.1% BSA, incubated at 4 degree celsius over... View More

Dynamin 2 Antibody in Immunohistochemistry (IHC)

Product Image Gallery

Immunogen image

Immunogen Image Dynamin 2 Polyclonal Antibody

产品信息

PA1-661

应用

建议稀释比

已发表文章

免疫印迹 (WB)

2-4 µg/mL

查看14篇已发表文章 14篇已发表文章

免疫组化 (IHC)

查看2篇已发表文章 2篇已发表文章

免疫组化（石蜡） (IHC (P))

查看1篇已发表文章 1篇已发表文章

免疫细胞化学 (ICC/IF)

1:100

查看3篇已发表文章 3篇已发表文章

产品规格

种属反应

Human, Mouse, Rat

已发表种属

Human, Mouse, Non-human primate, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic Peptide: C S(760) P T P Q R R P V S S I H P P G R P P A(779)

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein G

保存液

PBS with 1mg/mL BSA

内含物

0.05% sodium azide

保存条件

-20°C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

AB_2293040

产品详细信息

PA1-661 detects dynamin II (Dyn2) from rat, mouse, and human tissues and cells.

PA1-661 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~100 kDa protein representing Dyn2 from HeLa cell lysate.

PA1-661 immunizing peptide corresponds to amino acid residues 760-779 from human Dyn2. This sequence is 95% conserved in mouse and rat Dyn2. PA1-661 immunizing peptide (Cat. # PEP-056) is available for use in neutralization and control experiments.

靶标信息

Dynamins represent one of the subfamilies of GTP-binding proteins. These proteins share considerable sequence similarity over the N-terminal portion of the molecule, which contains the GTPase domain. Dynamins are associated with microtubules. They have been implicated in cell processes such as endocytosis and cell motility, and in alterations of the membrane that accompany certain activities such as bone resorption by osteoclasts. Dynamins bind many proteins that bind actin and other cytoskeletal proteins. Dynamins can also self-assemble, a process that stimulates GTPase activity. Five alternatively spliced transcripts encoding different proteins have been described. Additional alternatively spliced transcripts may exist, but their full-length nature has not been determined.

仅用于科研。不用于诊断过程。未经明确授权不得转售。