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Invitrogen
E-cadherin Polyclonal Antibody
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FIGURE: 1 / 20
E-cadherin Antibody (PA5-85088) in ICC/IF
Immunocytochemistry analysis of E-cadherin in HCT116 cells fixed in 4% paraformaldehyde at RT for 15 min. Samples were incubated with E-cadherin polyclonal antibody (Product # PA5-85088) using a dilution of 1:500 (green). Blue: Fluoroshield with DAPI. Scale bar = 10 μm.
Product Details
PA5-85088
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat,
Zebrafish
Published species
C. elegans,
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human E-Cadherin. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
0.14 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol, 1% BSA
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Keep as concentrated solution.
Predicted reactivity: Mouse (80%), Rat (83%), Dog (80%), Pig (83%), Bovine (82%).
Positive Control: MCF-7, HT-29.
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
E-Cadherin (epithelial cadherin) is a classical cadherin from the cadherin (alcium dependent adhesion protein) superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function is thought to contribute to progression in cancer by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. Identified transcript variants arise from mutation at consensus splice sites. E-cadherin plays a central role in the growth and development of cells by controlling tissue architecture, and maintenance of tissue integrity. In humans, E-cadherin is encoded by the CDH1 gene present on chromosome 16. Studies have demonstrated that reduction and/or loss of E-cadherin expression in carcinomas correlates positively with the potential of these tumors for invasion and metastasis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: ARC-1; cadherin 1, E-cadherin (epithelial); cadherin 1, epithelial; cadherin 1, type 1; cadherin 1, type 1, E-cadherin (epithelial); cadherin e; Cadherin-1; cadherin-E; calcium-dependent adhesion protein, epithelial; CAM 120/80; CD324; cell adhesion molecule; cell-CAM 120/80; E-Cadherin; E-cadherin 1; Ecadherin; Epithelial cadherin; hab; half baked; Uvomorulin
Gene Aliases: AA960649; Arc-1; CD324; CDH1; CDHE; E-cad; E-cadherin; ECAD; L-CAM; LCAM; Um; UVO
UniProt ID: (Human) P12830, (Mouse) P09803, (Rat) Q9R0T4, (Zebrafish) Q90Z37
Entrez Gene ID: (Human) 999, (Mouse) 12550, (Rat) 83502, (Zebrafish) 114424
glycoprotein binding
calcium ion binding
protein binding
beta-catenin binding
ankyrin binding
GTPase activating protein binding
gamma-catenin binding
cell adhesion molecule binding
cadherin binding involved in cell-cell adhesion
protein phosphatase binding
protein domain specific binding
metal ion binding
G-protein alpha-subunit binding
cadherin binding
cadherin
homophilic cell adhesion via plasma membrane adhesion molecules
synapse assembly
response to toxic substance
single organismal cell-cell adhesion
pituitary gland development
negative regulation of cell-cell adhesion
extracellular matrix disassembly
extracellular matrix organization
neuron projection development
adherens junction organization
response to drug
positive regulation of transcription factor import into nucleus
positive regulation of transcription, DNA-templated
cellular response to lithium ion
cellular response to indole-3-methanol
protein localization to plasma membrane
establishment of protein localization to plasma membrane
in utero embryonic development
trophectodermal cell differentiation
epithelial cell morphogenesis
cell adhesion
sensory perception of sound
calcium-dependent cell-cell adhesion via plasma membrane cell adhesion molecules
protein metabolic process
regulation of protein localization
regulation of water loss via skin
regulation of cysteine-type endopeptidase activity involved in apoptotic process
regulation of epithelial cell proliferation
negative regulation of epithelial cell proliferation
protein homooligomerization
intestinal epithelial cell development
salivary gland cavitation
regulation of branching involved in salivary gland morphogenesis
bicellular tight junction assembly
cellular response to amino acid stimulus
negative regulation of canonical Wnt signaling pathway
cochlea development
regulation of protein localization to cell surface
regulation of neuron migration
response to organic substance
gastrulation with mouth forming second
neuron migration
neural tube formation
cell-cell adhesion involved in mesendodermal cell migration
ectoderm development
germ cell migration
adherens junction maintenance
cell migration involved in gastrulation
epiboly involved in gastrulation with mouth forming second
cell-cell adhesion involved in gastrulation
cell-cell adhesion
cell-cell adhesion via plasma-membrane adhesion molecules
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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