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Invitrogen
EAP30 Polyclonal Antibody
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FIGURE: 1 / 1
EAP30 Antibody (PA5-87999) in WB
Western blot analysis of EAP30 in extracts of various cell lines. Samples were incubated with EAP30 Polyclonal antibody (Product # PA5-87999) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-87999
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-258 of human SNF8 (NP_0091722)
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
2.08 mg/mL
Amount
208 µg
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: MHRRGVGAGA IAKKKLAEAK YKERGTVLAE DQLAQMSKQL DMFKTNLEEF ASKHKQEIRK NPEFRVQFQD MCATIGVDPL ASGKGFWSEM LGVGDFYYEL GVQIIEVCLA LKHRNGGLIT LEELHQQVLK GRGKFAQDVS QDDLIRAIKK LKALGTGFGI IPVGGTYLIQ SVPAELNMDH TVVLQLAEKN GYVTVSEIKA SLKWETERAR QVLEHLLKEG LAWLDLQAPG EAHYWLPALF TDLYSQEITA EEAREALP; Positive Samples: 293T, Mouse brain; Cellular Location: Cytoplasm, Endosome membrane, Late endosome membrane, Nucleus
Target Information
ELL encodes an RNA polymerase II transcription factor that undergoes frequent translocation in acute myeloid leukemia (AML). In addition to its elongation activity, ELL contains a novel type of RNA polymerase II interaction domain that is capable of repressing polymerase activity in promoter-specific transcription. EAP30 is a subunit of the ELL complex. EAP30 can interact with ELL and derepress ELL's inhibitory activity in vitro.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ESCRT-II complex subunit VPS22; Vacuolar-sorting protein SNF8
Gene Aliases: D11Moh34; Snf8
UniProt ID: (Mouse) Q9CZ28
Entrez Gene ID: (Mouse) 27681
transcription, DNA-templated
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
transport
positive regulation of gene expression
regulation of multivesicular body size involved in endosome transport
protein transport
endocytic recycling
regulation of protein catabolic process
protein targeting to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway
early endosome to late endosome transport
positive regulation of protein catabolic process
regulation of protein complex stability
positive regulation of exosomal secretion
regulation of viral budding via host ESCRT complex
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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