Invitrogen

ERK1/ERK2 Polyclonal Antibody

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32 篇参考文献
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说明书
实验方案
问题与答案
技术支持
ERK1/ERK2 Polyclonal Antibody

产品信息

44-654G

应用
建议稀释比
已发表文章

免疫印迹 (WB)

1:1,000
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免疫组化（石蜡） (IHC (P))

1:10-1:100
-

免疫细胞化学 (ICC/IF)

1 µg/mL
-

免疫沉淀 (IP)

-
查看1篇已发表文章 1篇已发表文章

亲和纯化 (AP)

-
查看2篇已发表文章 2篇已发表文章

其他PubMed (Misc)

-
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产品规格

种属反应

Bovine, Human, Mouse, Rat

已发表种属

C. elegans, Dog, Human, Insect, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

The antibody was produced using a synthetic peptide derived from amino acids 317-339 within the C-terminal half of the human ERK1 protein; the sequence is conserved in human and rat
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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

纯化类型

Antigen affinity chromatography

保存液

Dulbecco's PBS, pH 7.3, with 1mg/mL BSA

内含物

0.05% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2533710

产品详细信息

For western blotting applications, we recommend using the antibody at a 1:1,000 starting dilution. Positive controls used in western blotting were human epidermoid carcinoma A431, mouse NIH3T3, and rat pheochromocytoma (PC12) cells.

靶标信息

ERK1 and ERK2 are widely expressed and are involved in the regulation of meiosis, mitosis, and postmitotic functions in differentiated cells. Many different stimuli, including growth factors, cytokines, virus infection, ligands for heterotrimeric guanine nucleotide-binding protein (G protein)-coupled receptors and transforming agents, activate the ERK1 and ERK2 pathways. When growth factors bind to the receptor tyrosine kinase, Ras interacts with Raf, the serine/threonine protein kinase and activates it as well. Once actived, Raf phosphorylates serine residue in 2 further kinases, MEK1/2, which in turn phosphorylates tyrosine/threonine in extracellular-signal regulated kinase (ERK) 1/2. Upon activation, the ERKs either phosphorylate a number of cytoplasmic targets or migrate to the nucleus, where they phosphorylate and activate a number of transcription factors such as c-Fos and Elk-1.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: ERK-1; ERK-2; erk1 erk2; ERK1b; ERT1; ERT2; Extracellular signal-regulated kinase 1; Extracellular signal-regulated kinase 2; extracellular signal-related kinase 1; extracellular-signal-regulated kinase 2; Insulin-stimulated MAP2 kinase; MAP kinase 1; MAP kinase 2; MAP kinase 3; MAP kinase isoform p42; MAP kinase isoform p44; MAPK 1; MAPK 2; MAPK 3; Microtubule-associated protein 2 kinase; mitogen activated protein kinase 1; mitogen-activated 3; Mitogen-activated protein kinase 1; Mitogen-activated protein kinase 2; Mitogen-activated protein kinase 3; MNK1; p42; p42 MAP Kinase; p42-MAPK; p44 MAP kinase; p44-ERK1; p44-MAPK; pp42/MAP kinase; protein tyrosine kinase ERK2

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基因别名: 9030612K14Rik; AA407128; AU018647; C78273; ERK; ERK-1; ERK-2; ERK1; ERK2; ERT1; ERT2; Esrk1; HS44KDAP; HUMKER1A; Mapk; MAPK1; MAPK2; MAPK3; MNK1; Mtap2k; p38; p40; p41; p41mapk; p42-MAPK; P42MAPK; p44; p44-ERK1; p44-MAPK; P44ERK1; P44MAPK; PRKM1; PRKM2; PRKM3

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UniProt ID: (Bovine) P46196, (Human) P28482, (Human) P27361, (Mouse) P63085, (Rat) P63086, (Rat) P21708, (Mouse) Q63844

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Entrez Gene ID: (Bovine) 327672, (Human) 5594, (Bovine) 531391, (Human) 5595, (Mouse) 26413, (Rat) 116590, (Rat) 50689, (Mouse) 26417

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功能
phosphotyrosine binding protein serine/threonine kinase activity MAP kinase kinase activity ATP binding RNA polymerase II carboxy-terminal domain kinase activity phosphatase binding identical protein binding DNA binding MAP kinase activity protein binding transcription factor binding kinase activity mitogen-activated protein kinase kinase kinase binding scaffold protein binding nucleotide binding protein kinase activity transferase activity transferase activity, transferring phosphorus-containing groups protein kinase binding non-receptor serine/threonine protein kinase
参与通路
activation of MAPK activity protein phosphorylation apoptotic process cellular response to DNA damage stimulus cell cycle positive regulation of gene expression positive regulation of peptidyl-threonine phosphorylation peptidyl-serine phosphorylation peptidyl-threonine phosphorylation cytosine metabolic process regulation of ossification regulation of cellular pH thyroid gland development regulation of protein stability lipopolysaccharide-mediated signaling pathway positive regulation of telomere maintenance via telomerase mammary gland epithelial cell proliferation cellular response to amino acid starvation cellular response to reactive oxygen species response to nicotine ERBB signaling pathway outer ear morphogenesis response to exogenous dsRNA negative regulation of cell differentiation thymus development T cell receptor signaling pathway B cell receptor signaling pathway stress-activated MAPK cascade positive regulation of telomerase activity Bergmann glial cell differentiation long-term synaptic potentiation face development lung morphogenesis trachea formation labyrinthine layer blood vessel development cardiac neural crest cell development involved in heart development ERK1 and ERK2 cascade response to epidermal growth factor cellular response to cadmium ion cellular response to tumor necrosis factor cellular response to granulocyte macrophage colony-stimulating factor stimulus cellular response to dopamine positive regulation of telomere capping MAPK cascade MAPK import into nucleus transcription, DNA-templated chemotaxis response to stress signal transduction chemical synaptic transmission axon guidance positive regulation of cell proliferation fibroblast growth factor receptor signaling pathway response to toxic substance regulation of phosphatidylinositol 3-kinase signaling viral process sensory perception of pain platelet activation positive regulation of cell migration regulation of stress-activated MAPK cascade Fc-epsilon receptor signaling pathway Fc-gamma receptor signaling pathway involved in phagocytosis response to estrogen positive regulation of translation positive regulation of transcription, DNA-templated regulation of sequence-specific DNA binding transcription factor activity regulation of cytoskeleton organization JAK-STAT cascade involved in growth hormone signaling pathway caveolin-mediated endocytosis regulation of Golgi inheritance regulation of cellular response to heat regulation of early endosome to late endosome transport positive regulation of protein phosphorylation transcription initiation from RNA polymerase I promoter protein complex assembly DNA damage induced protein phosphorylation phosphorylation arachidonic acid metabolic process BMP signaling pathway positive regulation of cyclase activity positive regulation of histone phosphorylation positive regulation of histone acetylation peptidyl-tyrosine autophosphorylation positive regulation of transcription from RNA polymerase II promoter cartilage development positive regulation of ERK1 and ERK2 cascade interleukin-1-mediated signaling pathway cellular response to mechanical stimulus positive regulation of xenophagy negative regulation of apolipoprotein binding organ morphogenesis response to lipopolysaccharide intracellular signal transduction cellular response to organic substance
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