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Invitrogen
Estrogen Receptor beta Polyclonal Antibody
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Estrogen Receptor beta Antibody (PA1-311) in WB
Western blot analysis was performed on nuclear enriched extracts (30 µg lysate) of LNCap (Lane 1), PC-3 (Lane 2), MCF-7 (Lane 3), MDA-MB-231 (Lane 4), T-47D (Lane 5) and HeLa (Lane 6). The blots were probed with Anti-Rabbit Polyclonal Antibody (Product # PA1-311, 1 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, HRP conjugate (Product # A270... View More
产品信息
PA1-311
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Non-human primate,
Sheep,
Rat
已发表种属
Bovine,
C. elegans,
Dog,
Hamster,
Human,
Mouse,
Non-human primate,
Pig,
Rat,
Sheep
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Synthetic peptide corresponding to residues A(55) E P Q K S P W C E A R S L E H(70) of rat ER beta.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS with 1mg/mL BSA
内含物
0.05% sodium azide
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
PA1-311 detects estrogen receptor (ER) beta from human, non-human primate, mouse, ovine and rat tissues.
PA1-311 has been successfully used in Western blot, immunocytochemistry and gel shift procedures. By Western blot, this antibody detects an ~55-62 kDa protein representing ER beta from rat brain homogenate. PA1-311 efficiently supershifts rat ER beta/ER response element (vitellogenin xenopus A2 gene).
The PA1-311 immunogen is a synthetic peptide corresponding to residues A(55) E P Q K S P W C E A R S L E H(70) of rat ER beta.
Recombinant ER beta expressed in baculovirus system (Cat. # RP-311 and RP-312) can be purchased for control experiments in Western blot and gel shift assays.
PA1-311 can be used with blocking peptide PEP-011.
靶标信息
Estrogen Receptor beta (ER beta, NR3A2) protein is approximately 55kD, though multiple isoforms ranging from 36-59kD have been described. Human ER beta was initially cloned and characterized from testis. Estrogen Receptors (ER) are members of the steroid/thyroid hormone receptor superfamily of nuclear receptors. The estrogen receptor is a ligand-activated transcription factor, that when bound to estrogen hormone, induces a conformational change that allows dimerization and binding to estrogen response elements (ERE) in DNA. When bound to EREs, ER can positively or negatively regulate gene transcription through the recruitment of coactivator or corepressor proteins. There are two different forms of the estrogen receptor, alpha and beta, encoded by separate genes (ESR1 and ESR2, respectively). Estrogen receptor beta (ER beta) binds estrogens with an affinity similar to that of estrogen receptor alpha. Some ER beta isoforms dominantly inhibit the activity of estrogen receptor alpha in reproductive tissues. ER beta is found widely in many tissues throughout the body and can act as a potent tumor suppressor, playing a crucial role in many cancer types such as prostate cancer, autism spectrum disorder, medullary thyroid carcinoma, De Quervain Disease and risk of cardiovascular disease.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: ER; ER beta; ER-beta; estrogen receptor 2 (ER beta); estrogen receptor 2 beta; Estrogen receptor beta; estrogen receptor beta 4; Nuclear receptor subfamily 3 group A member 2; oestrogen receptor beta; steroid receptor
基因别名: ER-BETA; ER[b]; ERB; Erb2; ERBETA; ESR-BETA; ESR2; ESRB; ESTRB; NR3A2
UniProt ID: (Human) O75584, (Sheep) Q9TU15, (Mouse) O08537, (Rat) Q62986
Entrez Gene ID: (Human) 2100, (Sheep) 443066, (Mouse) 13983, (Rat) 25149
core promoter sequence-specific DNA binding
DNA binding
transcription factor activity, sequence-specific DNA binding
steroid hormone receptor activity
transcription coactivator activity
RNA polymerase II transcription factor activity, ligand-activated sequence-specific DNA binding
steroid binding
protein binding
drug binding
zinc ion binding
enzyme binding
estrogen receptor activity
estrogen response element binding
RNA polymerase II transcription factor activity, estrogen-activated sequence-specific DNA binding
peroxisome proliferator activated receptor binding
receptor antagonist activity
estradiol binding
steroid hormone binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
lipid binding
hormone binding
sequence-specific DNA binding
metal ion binding
negative regulation of transcription from RNA polymerase II promoter
ovarian follicle development
behavioral fear response
neuron migration
transcription, DNA-templated
regulation of transcription, DNA-templated
transcription from RNA polymerase II promoter
brain development
learning or memory
negative regulation of cell proliferation
hormone-mediated apoptotic signaling pathway
intracellular steroid hormone receptor signaling pathway
intracellular estrogen receptor signaling pathway
response to testosterone
regulation of cell proliferation
positive regulation of apoptotic process
steroid hormone mediated signaling pathway
regulation of neuron apoptotic process
estrous cycle
positive regulation of epidermal growth factor receptor signaling pathway
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
negative regulation of behavior
negative regulation of smooth muscle cell proliferation
negative regulation of epithelial cell proliferation
positive regulation of sequence-specific DNA binding transcription factor activity
Sertoli cell proliferation
uterus development
vagina development
negative regulation of cell death
prostate gland epithelium morphogenesis
epithelial cell maturation involved in prostate gland development
negative regulation of androgen receptor signaling pathway
positive regulation of ERK1 and ERK2 cascade
negative regulation of neuron death
negative regulation of feeding behavior
negative regulation of reactive oxygen species metabolic process
transcription initiation from RNA polymerase II promoter
signal transduction
cell-cell signaling
response to water deprivation
response to activity
response to insecticide
cerebellum development
amygdala development
hypothalamus development
negative regulation of cell growth
prostate gland development
response to nutrient levels
response to genistein
cellular response to drug
response to estrogen
response to human chorionic gonadotropin
response to ethanol
positive regulation of vasodilation
Sertoli cell development
cellular response to magnetism
cellular response to estradiol stimulus
response to dexamethasone
extracellular negative regulation of signal transduction
response to salt
response to bisphenol A
male gonad development
female gonad development
response to hormone
response to organic cyclic compound
response to estradiol
response to drug
cellular response to organic cyclic compound
