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Invitrogen
FIS1 Polyclonal Antibody
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图: 1 / 18
FIS1 Antibody (PA5-22142) in ICC/IF
FIS1 Polyclonal Antibody detects FIS1 protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37oC for 30 min. Green: FIS1 protein stained by FIS1 Polyclonal Antibody (Product # PA5-22142) diluted at 1:2,000. Red: MitoTrackerR Red CMXRos, a mitochondria tracker. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
产品信息
PA5-22142
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
已发表种属
C. elegans,
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Full length human FIS1 Recombinant protein.
偶联物
形式
Liquid
浓度
1.4 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7, with 20% glycerol
内含物
0.025% ProClin 300
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Recommended positive controls: 293T, A431, HeLa, HepG2, Raji, Mouse brain, mouse liver, rat brain.
Predicted reactivity: Mouse (97%), Rat (97%), Rhesus Monkey (100%), Bovine (97%).
靶标信息
Fis1, a 17KDa integral membrane protein, is a novel member of mitochondrial fission machinery. It consists of a central leucine-zipper domain, a coiled-coil region, a tetratricopeptide repeat domain and a C-terminal transmembrane domain. It is a human orthologue of Fis1p (S.cerevisiae) and is localized in the outer mitochondrial membrane. Fis1 acts as a pro-apoptotic factor and plays a vital role in the regulation of mitochondrial fission. It interacts with DLP1 and Mdv1p and facilitates Dnm1p recruitment to the functional fission complex, thereby inducing apoptosis. Role of Fis1 has also been implicated in growth and division of peroxisomes. Loss of Fis1 inhibits apoptosis and results in elongation of mammalian mitochondria.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: FIS1 homolog; fis1 {ECO:0000250|UniProtKB:Q9Y3D6}; fission 1 (mitochondrial outer membrane) homolog; H_NH0132A01.6; hFis1; Mitochondrial fission 1 protein; mitochondrial fission molecule; rFis1; tetratricopeptide repeat domain 11; Tetratricopeptide repeat protein 11; TPR repeat protein 11
基因别名: 2010003O14Rik; CGI-135; FIS1; TTC11
UniProt ID: (Human) Q9Y3D6, (Rat) P84817, (Mouse) Q9CQ92
Entrez Gene ID: (Human) 51024, (Rat) 288584, (Mouse) 66437
mitochondrial fission
mitophagy
release of cytochrome c from mitochondria
protein targeting to mitochondrion
positive regulation of cytosolic calcium ion concentration
mitochondrial fusion
regulation of mitochondrion organization
peroxisome fission
negative regulation of endoplasmic reticulum calcium ion concentration
calcium-mediated signaling using intracellular calcium source
positive regulation of cysteine-type endopeptidase activity involved in apoptotic process
mitochondrial fragmentation involved in apoptotic process
protein homooligomerization
positive regulation of mitochondrial calcium ion concentration
mitochondrion morphogenesis
positive regulation of mitochondrial fission
positive regulation of protein targeting to membrane
positive regulation of intrinsic apoptotic signaling pathway
apoptotic process
