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Invitrogen
FITC/5-FAM/6-FAM Recombinant Rabbit Monoclonal Antibody (6R1B5)
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FITC/5-FAM/6-FAM Antibody (MA5-55270) in WB
Western blot analysis of recombinant FITC conjugated goat IG protein using FITC/5-FAM/6-FAM Recombinant Monoclonal Antibody (Product # MA5-55270) at 1:2,000 dilution followed by a HRP-conjugated Goat anti-Rabbit IgG (H+L) secondary antibody at 1:10,000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
产品信息
MA5-55270
产品规格
种属反应
Chemical
宿主/亚型
Rabbit
/ IgG
Expression System
HEK293 cells
分类
Recombinant Monoclonal
类型
Antibody
克隆号
6R1B5
抗原
Chemical compounds corresponding to FITC/5-FAM/6-FAM.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein A
保存液
PBS, pH 7.3, with 50% glycerol, 0.05% BSA
内含物
0.05% ProClin 300
保存条件
-20°C, Avoid Freeze/Thaw Cycles
RRID
产品详细信息
Immunogen Sequence: FITC conjugated to KLH
靶标信息
FITC (Fluorescein Isothiocyanate), 5-FAM (5-Carboxyfluorescein), and 6-FAM (6-Carboxyfluorescein) are commonly used gene targets for fluorescent labeling in molecular biology research. These dyes belong to the fluorescein family and emit a green fluorescence when excited by specific wavelengths of light. FITC, 5-FAM, and 6-FAM are utilized in various techniques such as fluorescence in situ hybridization (FISH), flow cytometry, immunofluorescence, and DNA sequencing. By attaching these dyes to gene-specific probes or primers, researchers can visualize and detect gene expression levels, identify specific DNA or RNA sequences, and study cellular processes. The compatibility of FITC, 5-FAM, and 6-FAM with different imaging systems and their bright green fluorescence make them valuable tools in molecular biology, aiding in the understanding of gene function and cellular dynamics.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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