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Invitrogen
FITC Recombinant Mouse Monoclonal Antibody (NAWESLEE), Alexa Fluor™ Plus 647
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图: 1 / 6
FITC Antibody (740178MP647) in ICC/IF
Immunofluorescence analysis of FITC was performed using FITC Recombinant Mouse Monoclonal Antibody (NAWESLEE), Alexa Fluor™ Plus 647 (Product # 740178MP647) on HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 min, permeabilized with 0.5% Triton™ X-100 for 15 min and blocked with 2% BSA for 1 hr at RT. The cells were stained with Anti-Ki-67 FITC (Product # 11-5698-82) in 0.1% BSA, incubated at 4ºC overnight, followed by FITC Recombinant Mouse Monoclonal Antibody (NAWESLEE), Alexa Fluor™ Plus 647 (Product # 740178MP647, 1:500... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
产品信息
740178MP647
产品规格
种属反应
Chemical
宿主/亚型
Mouse
/ IgG2a, kappa
Expression System
Expi293
分类
Recombinant Monoclonal
类型
Antibody
克隆号
NAWESLEE
偶联物
Alexa Fluor™ Plus 647
Alexa Fluor™ Plus 647
Alexa Fluor™ Plus 647
激发/发射光谱
658/675 nm
查看光谱
形式
Liquid
浓度
1 mg/mL
规格
50 µg
纯化类型
Protein A/G
保存液
proprietary buffer, pH 6.3-7.3
内含物
0.008% Bromonitrodioxane, 0.008% Methylisothiazolone
保存条件
4°C, store in dark, DO NOT FREEZE!
运输条件
Wet ice
产品详细信息
Alexa Fluor™ Plus recombinant antibodies are conjugated using new, proprietary dye chemistry so you can generate stunning data. Alexa Fluor™ Plus antibodies represent an advancement in fluorescent conjugate technology. Alexa Fluor™ Plus antibodies provide brighter signal compared to leading Alexa Fluor™ antibodies, providing you with better signal-to-noise for your critical experiments. These antibodies show better specificity and lot-to-lot consistency as these are recombinant antibodies, generated by cloning specific genes for the desired antibodies into an expression vector and expressed in vitro.
Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment. This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining.
It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 658 nm; Emission: 675 nm; Laser: Red Laser
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
FITC (fluorescein isothiocyanate) is a fluorochrome dye that absorbs ultraviolet or blue light causing molecules to become excited and emit a visible yellow-green light. This emission ceases upon removal of the light causing the excitation. Fluorochrome labeling provides rapid, accurate localization of antigen-antibody interaction when one of the reactants is part of a cell, tissue or other biological structure. FITC is a commonly used marker for antibodies in immunofluorescent techniques since the conjugation of FITC to proteins is relatively easy and does not, in general, destroy the biological activity of the labeled protein. FITC is widely used as a hapten to label different proteins. Anti-FITC antibodies can be used for isolating FITC labeled cells by magnetic microbeads based sorting, as well as removal of background signal.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Fluorescein
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