Invitrogen

FKBP12 Polyclonal Antibody

Name: FKBP12 Polyclonal Antibody
Brand: Invitrogen
SKU: PA1-026A

高级验证

16个已发表图片

43 篇参考文献

查看其他16个FKBP12抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

FKBP12 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

图: 1 / 21

FKBP12 Antibody (PA1-026A) in IHC (P)

Immunohistochemistry was performed on cancer biopsies of deparaffinized human Colon carcinoma tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with a rabbit poly... View More

FKBP12 Antibody in Immunohistochemistry (Frozen) (IHC (F))

FKBP12 Antibody in Immunohistochemistry (IHC)

FKBP12 Antibody in Western Blot, Immunohistochemistry (WB, IHC)

Product Image Gallery

Immunogen image

Immunogen Image FKBP12 Polyclonal Antibody

产品信息

PA1-026A

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1 µg/mL

查看32篇已发表文章 32篇已发表文章

免疫组化 (IHC)

查看6篇已发表文章 6篇已发表文章

免疫组化（石蜡） (IHC (P))

2 µg/mL

免疫组化（冰冻） (IHC (F))

查看1篇已发表文章 1篇已发表文章

免疫细胞化学 (ICC/IF)

查看2篇已发表文章 2篇已发表文章

免疫沉淀 (IP)

Assay-dependent

查看1篇已发表文章 1篇已发表文章

亲和纯化 (AP)

查看1篇已发表文章 1篇已发表文章

产品规格

种属反应

Human, Mouse

已发表种属

C. elegans, Human, Mouse, Pig, Protozoa, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptide corresponding to the N-terminal residues G(1) V Q V E T I S P G D G R(13) of human FKBP12.

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS with 1mg/mL BSA

内含物

0.05% sodium azide

保存条件

-20°C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

AB_2102731

产品详细信息

PA1-026A detects FK506 binding protein 12 kDa (FKBP12) in human and mouse tissues.

PA1-026A has been successfully used in Western blot, immunohistochemistry, and immunoprecipitation procedures. By Western blot, this antibody detects a 12 kDa protein representing FKBP12 from rat heart homogenate.

The PA1-026A immunogen is a synthetic peptide corresponding to the N-terminal residues G(1) V Q V E T I S P G D G R(13) of human FKBP12. PA1-026A immunizing peptide (Cat. # PEP-012) is available for use in neutralization and control experiments.

靶标信息

FKBP12 is a member of the immunophilin protein family, which play a role in immunoregulation and basic cellular processes involving protein folding and trafficking. The protein is a cis-trans prolyl isomerase that binds the immunosuppressants FK506 and rapamycin. It interacts with several intracellular signal transduction proteins including type I TGF-beta receptor. It also interacts with multiple intracellular calcium release channels, and coordinates multi-protein complex formation of the tetrameric skeletal muscle ryanodine receptor. In mouse, deletion of this homologous gene causes congenital heart disorder known as noncompaction of left ventricular myocardium.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: 12 kDa FK506-binding protein; 12 kDa FKBP; calstabin 1; Calstabin-1; EC 5.2.1.8; FK506 binding protein 1A, 12kDa; FK506 binding protein12; FK506-binding protein 1; FK506-binding protein 12; FK506-binding protein 1A; FK506-binding protein 1A (12kD); FK506-binding protein, T-cell, 12-kD; FKBP-1A; FKBP12-Exip3; Immunophilin FKBP12; Peptidyl-prolyl cis-trans isomerase FKBP1A; PPIase FKBP1A; protein kinase C inhibitor 2; Rotamase

查看更多收起

基因别名: Fkbp; FKBP-12; FKBP-1A; FKBP1; FKBP12; FKBP1A; PKC12; PKCI2; PPIASE

查看更多收起

UniProt ID: (Human) P62942, (Mouse) P26883

查看更多收起

Entrez Gene ID: (Human) 2280, (Mouse) 14225

查看更多收起

功能