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Invitrogen
FOXL2 Polyclonal Antibody
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FOXL2 Antibody (PA1-802) in ICC/IF
Immunofluorescence analysis of FOXL2 was done on 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with FOXL2 Rabbit Polyclonal Antibody (Product # PA1-802) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at... View More
产品信息
PA1-802
应用
建议稀释比
已发表文章
产品规格
种属反应
Hamster,
Human,
Mouse
已发表种属
Human,
Mouse
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Synthetic Peptide corresponding to residues M(1) M A S Y P E P E D T A G T(14) of mouse FoxL2.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS with 1mg/mL BSA
内含物
0.05% sodium azide
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
PA1-802 detects FoxL2 protein in mouse and hamster samples.
PA1-802 has successfully been used in Western blot , immunofluorescence and immunohistochemistry procedures. By Western blot, this antibody detects an ~39 kDa protein representing FozL2 in CHO whole cell lysate.
The PA1-802 immunogen is a synthetic Peptide corresponding to residues M(1) M A S Y P E P E D T A G T(14) of mouse FoxL2. This sequence is completely conserved in mouse and rat and 92% conserved in human. This peptide (Cat. # PEP-238) is available for use in neutralization and control experiments.
靶标信息
FOXL2 is a winged helix/forkhead transcription factor that is highly conserved in human, goat, mouse, and certain aquatic species. Given its cross-species conservation, it appears to play a critical role evolutionarily, likely in ovarian differentiation in mammals and in ovarian somatic cell differentiation and in further follicle development and/or maintenance. Immunohistochemical data shows that FOXL2 is a nuclear protein specifically expressed in eyelids and in fetal and adult ovarian follicular cells. It does not undergo any major posttranslational modifications. FOXL2 is also found to be associated with blepharo-phimosis/ptosis/epicanthus inverses syndrome (BPES). There are two forms of BPES. Type I BPES shows eyelid abnormal-ities that are associated with ovarian failure. In type II BPES, only eyelid defects are found. Studies have shown that FOXL2 is expressed in the mesenchyme of developing mouse eyelids and in adult ovarian follicles.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: Blepharophimosis; epicanthus inversus; Forkhead box protein L2; forkhead transcription factor FOXL2; LUSTR2; MGC14393; P-Frk; Pituitary forkhead factor; putative transcription factor foxl2
基因别名: AU045128; BPES; BPES1; FOXL2; PFRK; PINTO; POF3
UniProt ID: (Human) P58012, (Mouse) O88470
Entrez Gene ID: (Human) 668, (Mouse) 26927
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
DNA binding
transcription factor activity, sequence-specific DNA binding
protein binding
estrogen receptor binding
ubiquitin conjugating enzyme binding
cysteine-type endopeptidase regulator activity involved in apoptotic process
sequence-specific DNA binding
winged helix/forkhead transcription factor
negative regulation of transcription from RNA polymerase II promoter
ovarian follicle development
oocyte growth
extraocular skeletal muscle development
apoptotic DNA fragmentation
transcription from RNA polymerase II promoter
single fertilization
female somatic sex determination
cell differentiation
positive regulation of luteinizing hormone secretion
menstruation
positive regulation of apoptotic process
positive regulation of cysteine-type endopeptidase activity involved in apoptotic process
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
positive regulation of follicle-stimulating hormone secretion
embryonic eye morphogenesis
granulosa cell differentiation
uterus development
transcription, DNA-templated
regulation of transcription, DNA-templated
