Invitrogen

Fumarase Polyclonal Antibody

Name: Fumarase Polyclonal Antibody
Brand: Invitrogen
SKU: PA5-79265

查看其他33个Fumarase抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (11)

Fumarase Antibody in Immunocytochemistry (ICC/IF)

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Fumarase Antibody (PA5-79265) in ICC/IF

Immunocytochemistry analysis of FH using anti-FH antibody (Product # PA5-79265) . FH was detected in a section of PC-3 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and then incubated with 2μg/mL rabbit anti-FH antibody (Product # PA5-79265) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize usin... View More

Fumarase Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Fumarase Antibody in Flow Cytometry (Flow)

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Immunogen image

Immunogen Image Fumarase Polyclonal Antibody

产品信息

PA5-79265

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.1-0.5 µg/mL

免疫组化（石蜡） (IHC (P))

0.5-1 µg/mL

免疫组化（冰冻） (IHC (F))

0.5-1 µg/mL

免疫细胞化学 (ICC/IF)

2 µg/mL

流式细胞分析 (Flow)

1-3 µg/1x10^6 cells

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A synthetic peptide corresponding to a sequence of human FH (YDKAAKIAKTAHKNGSTLKETAIELGYLTAEQFDEWVKPKDMLGPK).

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

500 µg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS with 4mg trehalose

内含物

0.05mg sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_2746381

产品详细信息

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

Positive Control - WB: rat lymphaden tissue, rat small intestine tissue, rat gaster tissue, mouse kidney tissue, mouse testis tissue, mouse gaster tissue, human K562 whole cell, human U937 whole cell, human colon cancer tissue, human lung cancer tissue, human placenta tissue, mouse small intestine tissue, human mammary cancer tissue. ICC/IF: PC-3 cell. Flow: A431 cell, PC-3 cell.

靶标信息

The protein encoded by this gene is an enzymatic component of the tricarboxylic acid cycle, or Krebs cycle, and catalyzes the formation of L-malate from fumarate. It exists in both a cytosolic form and an N-terminal extended form, differing only in the translation start site used. The N-terminal extended form is targeted to the mitochondrion, where the removal of the extension generates the same form as in the cytoplasm. It is similar to some thermostable class II fumarases and functions as a homotetramer. Mutations in this gene can cause fumarase deficiency and lead to progressive encephalopathy.

仅用于科研。不用于诊断过程。未经明确授权不得转售。