Invitrogen

Furin Polyclonal Antibody

Name: Furin Polyclonal Antibody
Brand: Invitrogen
SKU: PA1-062

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31个已发表图片

55 篇参考文献

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说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

Furin Antibody in Immunocytochemistry (ICC/IF)

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Furin Antibody (PA1-062) in ICC/IF

Immunofluorescence analysis of Furin was performed using 70% confluent log phase BeWo cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with Furin Polyclonal Antibody (Product # PA1-062) at 2 µg/mL dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A327... View More

Furin Antibody in Western Blot, Immunocytochemistry, Immunoprecipitation (WB, ICC/IF, IP)

Furin Antibody in Immunohistochemistry (IHC)

Furin Antibody in Western Blot, Immunoprecipitation (WB, IP)

Furin Antibody in Immunoprecipitation (IP)

Product Image Gallery

Furin Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell lines and tissues owing to their inherent genetic constitution. Relative expression of Furin was observed in HEK-293, HaCaT, BeWo, SK-O-V3, Mouse Liver and Rat Liver but not in Daudi and Ramos which are known to be low expressing cell lines (DOI: 10.1099/0022-1317-75-10-2821) using Anti-Furin Polyclonal Antibody (Product # PA1-062) in Western Blot. {RE}

Immunogen image

Immunogen Image Furin Polyclonal Antibody

产品信息

PA1-062

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

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免疫组化 (IHC)

查看3篇已发表文章 3篇已发表文章

免疫细胞化学 (ICC/IF)

2 µg/mL

查看24篇已发表文章 24篇已发表文章

免疫沉淀 (IP)

1:100

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中和实验 (Neu)

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产品规格

种属反应

Dog, Human, Mouse, Rat

已发表种属

C. elegans, Dog, Hamster, Human, Mouse, Non-human primate, Pig, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptide corresponding to residues R(780) G E R T A F I K D Q S A L(793) of human Furin.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

2 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS with 1mg/mL BSA

内含物

0.05% sodium azide

保存条件

-20°C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

AB_2105077

产品详细信息

PA1-062 detects furin convertase from canine and mouse cells as well as transfected human furin. This antibody does not detect endogenous furin from BSC-40, HeLa, J774A.1 BPAEC, or CHO cells, or from rat skeletal muscle, spleen, kidney, ovary, testes, heart, or brain tissue.

PA1-062 has been successfully used in Western blot, immunocytochemistry, immunoprecipitation, and immunofluorescence procedures. By Western blot, this antibody detects an ~100 kDa protein representing furin in BSC-40 cells transfected with the human furin gene. In addition, PA1-062 detects human furin transfected into 3T3 cells. Immunofluorescence staining of endogenous furin convertase in 3T3 cells with PA1-062 yields a pattern consistent with paranuclear staining.

The PA1-062 immunogen is a synthetic peptide corresponding to residues R(780) G E R T A F I K D Q S A L(793) of human Furin. The PA1-062 immunizing peptide (Cat. # PEP-010) is available for use in neutralization and control experiments.

靶标信息

Furin is a membrane-associated, calcium-dependent, serine protease that belongs to the subtilisin-like prohormone convertase (PC) family. Members of this family of cellular enzymes cleave most prohormones and neuropeptide precursors. Numerous other cellular proteins, some viral proteins, and bacterial toxins that are transported by the constitutive secretory pathway are also targeted for maturation by PCs. Furin and other PC family members share structural similarities which include a heterogeneous ~10 kDa amino-terminal proregion, a highly conserved ~55 kDa subtilisin-like catalytic domain, and carboxyl-terminal domain that is heterogeneous in length and sequence. These enzymes become catalytically active following proregion cleavage within the appropriate cellular compartment. Furin is the only known PC to possess a transmembrane domain. Cleavage of target proteins occurs at the carboxyl-terminus of the furin consensus sequence, RX(K/R)R. It has been shown that the acidic peptide sequence, C771PSDSEEDEG780, localizes furin to the trans-Golgi-network. Phosphorylation of serine residues within this region modulates intracellular routing of furin protein. An additional signaling domain includes the tetrapeptide sequence, Y759KGL762, which directs internalization from the cell surface.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: dibasic processing enzyme; Dibasic-processing enzyme; FES upstream region; Furin; furin (paired basic amino acid cleaving enzyme); furin, membrane associated receptor protein; PACE; Paired basic amino acid cleaving enzyme (furin); paired basic amino acid residue cleaving enzyme; Paired basic amino acid residue-cleaving enzyme; Prohormone convertase 3; proprotein convertase subtilisin/kexin type 3; Proprotein convertase subtilisin/kexin type 3 (paired basic amino acid cleaving enzyme, furin, membrane associated receptor protein); proprotein convertase subtilisin/kexin type3; subtilisin pro-protein processing enzyme

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基因别名: 9130404I01Rik; FUR; FURIN; PACE; PCSK3; SPC1

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UniProt ID: (Human) P09958, (Rat) P23377, (Mouse) P23188

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Entrez Gene ID: (Dog) 488746, (Human) 5045, (Rat) 54281, (Mouse) 18550

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