Invitrogen

GATA4 Polyclonal Antibody

3个已发表图片

12 篇参考文献

查看其他24个GATA4抗体

问题与答案

问题与答案

抗体检测数据 (13)
已发表图片 (3)

GATA4 Antibody in Immunocytochemistry (ICC/IF)

GATA4 Antibody in Immunocytochemistry (ICC/IF)

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GATA4 Antibody (PA1-102) in ICC/IF

Immunofluorescent analysis of GATA4 (green) in embryoid body endoderm generated from Gibco ® Human Episomal iPSC Line grown on Geltrex® in Essential 8TM Medium. After 2 weeks in culture, EB were dissociated with TrypLETM and re-plated onto Geltrex®-coated multi-well plates. Cells were fixed, permeabilized and blocked for immunostaining. Cells were stained with a GATA4 polyclonal antibody (Product # PA1-102) at a dilution of 1:100 in 3% BSA/PBS blocking buffer overnight at 4°C, and then incubated with Alexa Fluor® 488 donkey anti rabbit ant... View More

GATA4 Antibody in Immunocytochemistry (ICC/IF)

GATA4 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

GATA4 Antibody in Western Blot (WB)

GATA4 Antibody in Flow Cytometry (Flow)

GATA4 Antibody in Immunocytochemistry (ICC/IF)

GATA4 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

GATA4 Antibody in Immunocytochemistry (ICC/IF)

GATA4 Antibody in Immunocytochemistry (ICC/IF)

GATA4 Antibody in Immunocytochemistry (ICC/IF)

GATA4 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

GATA4 Antibody in Western Blot (WB)

GATA4 Antibody in Flow Cytometry (Flow)

GATA4 Antibody in Flow Cytometry (Flow)

GATA4 Antibody in Immunocytochemistry (ICC/IF)

GATA4 Antibody in Immunocytochemistry (ICC/IF)

GATA4 Antibody in Western Blot (WB)

Product Image Gallery

GATA4 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of GATA4 (green) in embryoid body endoderm generated from Gibco ® Human Episomal iPSC Line grown on Geltrex® in Essential 8TM Medium. After 2 weeks in culture, EB were dissociated with TrypLETM and re-plated onto Geltrex®-coated multi-well plates. Cells were fixed, permeabilized and blocked for immunostaining. Cells were stained with a GATA4 polyclonal antibody (Product # PA1-102) at a dilution of 1:100 in 3% BSA/PBS blocking buffer overnight at 4°C, and then incubated with Alexa Fluor® 488 donkey anti rabbit antibody (Product # A-21206) at a 1:500 dilution in conjunction with NucBlue® Fixed Cell Ready Probes® Reagent. After another 3 washes, images were taken on EVOS®Floid® Cell Imaging system at 10X magnification.

GATA4 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of GATA4 showing positive staining in the nucleus of paraffin-treated Human small intestine (right) compared with a negative control in the absence of primary antibody (left). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a GATA4 polyclonal antibody (Product # PA1-102) diluted by 3% BSA-PBS at a dilution of 1:200 overnight at 4°C in a humidified chamber. Tissues were washed extensively PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

GATA4 Antibody in Western Blot (WB) — Western blot analysis was performed on whole cell extracts (20 µg lysate) of SH-SY5Y (Lane 1) and Hep G2 (Lane 2). The blots were probed with Anti-GATA4 Rabbit Polyclonal Antibody (Product # PA1-102, 1:500-1:2000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.4 µg/mL, 1:2500 dilution). A 46 kDa band corresponding to GATA4 was observed across the cell lines tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 12 % Bis-Tris gel (Product # NP0342BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).

GATA4 Antibody in Flow Cytometry (Flow) — Flow cytometry analysis of GATA4 in Hela cells compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/mL, fixed with 2% paraformaldehyde and washed with PBS. Cells were penetrated by dropping the supernatant, adding 90% methanol and incubated for 10 minutes at room temperature. Follwing penetration, cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with a GATA4 polyclonal antibody (Product # PA1-102) at a dilution of 2 µg/test for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated goat anti-rabbit IgG (H+L) secondary antibody and re-suspended in PBS for FACS analysis.

GATA4 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of GATA4 using anti-GATA4 polyclonal antibody (Product # PA1-102) in HeLa cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were blocked with Blocker BSA (Product # 37525) for 15 minutes at room temperature and probed with a rabbit polyclonal antibody recognizing GATA4 (Product # PA1-102), at a dilution of 1:50 for 1 hour at room temperature. Cells were then washed with PBS and incubated with DyLight 488 (green) goat-anti-rabbit secondary antibody (Product # 35552) at a dilution of 1:400 for 30 minutes at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ArrayScan at 20X magnification.

GATA4 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of GATA4 showing positive staining in the nucleus of paraffin-treated Mouse pancreas tissue (right) compared with a negative control in the absence of primary antibody (left). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a GATA4 polyclonal antibody (Product # PA1-102) diluted by 3% BSA-PBS at a dilution of 1:500 overnight at 4°C in a humidified chamber. Tissues were washed extensively PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

GATA4 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of GATA4 (green) showing positive staining in the nucleus of NIH-3T3 cells (right) compared with a negative control in the absence of primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes, blocked with 3% BSA-PBS for 30 minutes at room temperature and probed with a GATA4 polyclonal antibody (Product # PA1-102) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 488-conjugated goat-anti-rabbit IgG secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with DAPI for 5-10 minutes in the dark. Images were taken at a magnification of 60x.

GATA4 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of GATA4 (green) showing positive staining in the nucleus of HepG2 cells (right) compared with a negative control in the absence of primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes, blocked with 3% BSA-PBS for 30 minutes at room temperature and probed with a GATA4 polyclonal antibody (Product # PA1-102) in 3% BSA-PBS at a dilution of 1:200 and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 488-conjugated goat-anti-rabbit IgG secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with DAPI for 5-10 minutes in the dark. Images were taken at a magnification of 60x.

GATA4 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of GATA4 (green) showing positive staining in the nucleus of Hela cells (right) compared with a negative control in the absence of primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes, blocked with 3% BSA-PBS for 30 minutes at room temperature and probed with a GATA4 polyclonal antibody (Product # PA1-102) in 3% BSA-PBS at a dilution of 1:200 and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 488-conjugated goat-anti-rabbit IgG secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with DAPI for 5-10 minutes in the dark. Images were taken at a magnification of 60x.

GATA4 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of GATA4 showing positive staining in the nucleus of paraffin-treated Human pancreas tissue (right) compared with a negative control in the absence of primary antibody (left). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a GATA4 polyclonal antibody (Product # PA1-102) diluted by 3% BSA-PBS at a dilution of 1:200 overnight at 4°C in a humidified chamber. Tissues were washed extensively PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

GATA4 Antibody in Western Blot (WB) — Western blot analysis of GATA4 was performed by loading 40 µg of HeLa nuclear fraction (NF) (Lane 1), HEK293T control (Lane 2), and HEK293T GATA4 over-expression lysate (Lane 3) onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for 1 hour. Membranes were probed with a rabbit polyclonal antibody recognizing GATA4 (Product # PA1-102) at a dilution of 1:1000 overnight at 4°C on a rocking platform. Membranes were washed in TBS-0.1%Tween 20 and probed with a goat anti-rabbit-HRP secondary antibody (Product # 31460) at a dilution of 1:20,000 for one hour. Membranes were washed and chemiluminescent detection performed using Super Signal West Pico (Product # 34078). Nuclear fractions were generated using the NE-PER kit (Product # 78833).

GATA4 Antibody in Flow Cytometry (Flow) — Flow cytometry analysis of GATA4 in NIH/3T3 cells compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/mL, fixed with 2% paraformaldehyde and washed with PBS. Cells were penetrated by dropping the supernatant, adding 90% methanol and incubated for 10 minutes at room temperature. Follwing penetration, cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with a GATA4 polyclonal antibody (Product # PA1-102) at a dilution of 2 µg/test for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated goat anti-rabbit IgG (H+L) secondary antibody and re-suspended in PBS for FACS analysis.

GATA4 Antibody in Flow Cytometry (Flow) — Flow cytometry analysis of GATA4 in HepG2 cells compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/mL, fixed with 2% paraformaldehyde and washed with PBS. Cells were penetrated by dropping the supernatant, adding 90% methanol and incubated for 10 minutes at room temperature. Follwing penetration, cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with a GATA4 polyclonal antibody (Product # PA1-102) at a dilution of 2 µg/test for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated goat anti-rabbit IgG (H+L) secondary antibody and re-suspended in PBS for FACS analysis.

GATA4 Antibody in Immunocytochemistry (ICC/IF) — FIGURE 4 Confocal imaging to assess the expression of cardiac markers in human induced-pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) cultured on the aligned coaxial nanofiber patch. (A-F) Confocal images showing the expression of alpha-SA and GATA4 in hiPSC-CMs cultured on aligned coaxial patches. (G,H) Confocal images showing the expression of TnT and Cx-43 in hiPSC-CMs cultured on aligned coaxial patches. (J,K) Confocal images showing the expression of TnT and Nkx2.5 in hiPSC-CMs cultured on aligned coaxial patches. (C,F) Z-stack images showing the distribution of the hiPSC-CMs through the depth of the coaxial patches. (D,J) Cross-section images of the coaxial patches showing the distribution of hiPSC-CMs in the patch. (I) Confocal image of a single hiPSC-CM cultured on an aligned coaxial patch.

GATA4 Antibody in Immunocytochemistry (ICC/IF) — FIGURE 1 hCPCs morphology and cardiac gene expression under normoxic and hypoxic microenvironments. Human CPCs were cultured under 21, 5, and 1% O 2 for 48 h. DIC imaging shows the typical morphology of cells, which is unchanged under hypoxia. Immunofluorescent staining for cardiac lineage markers NKX-2.5 (green, nuclear), GATA-4 (green, nuclear), and c-kit (green) showed their expression was maintained under all oxygen conditions. Nuclei are stained blue and F-actin is stained red.

GATA4 Antibody in Western Blot (WB) — Published figure using GATA4 polyclonal antibody (Product # PA1-102) in Western Blot

Immunogen image

GATA4 Polyclonal Antibody

Immunogen Image GATA4 Polyclonal Antibody

产品信息

PA1-102

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

查看3篇已发表文章 3篇已发表文章

免疫组化 (IHC)

-

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免疫组化（石蜡） (IHC (P))

1:100-1:1,000

-

免疫细胞化学 (ICC/IF)

1:50-1:200

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流式细胞分析 (Flow)

3-5 µg/1x10^6 cells

-

亲和纯化 (AP)

-

查看1篇已发表文章 1篇已发表文章

产品规格

种属反应

Human, Mouse

已发表种属

C. elegans, Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Pooled peptides: RKEGIQTRKRKPKNLNKSK & SKQDSWNSLVLADSHGDIITA

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS with 1mg/mL BSA, 30% glycerol

内含物

0.05% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

PA1-102 has been successfully used in Western blot, FACS, immunohistochemistry (paraffin) and immunofluorescence applications with human and mouse samples. PA1-102 can be used for immunofluorescence analysis of GATA4 in the endoderm derived from human iPSCs.

靶标信息

GATA4 is a member of the GATA family of zinc-finger transcription factors. Members of this family recognize the GATA motif which is present in the promoters of many genes. This protein is thought to regulate genes involved in embryogenesis and in myocardial differentiation and function. Mutations in the gene encoding GATA4 have been associated with cardiac septal defects.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: GAT4; GATA-binding factor 4; GATA-binding protein 4; MGC126629; Transcription factor GATA-4

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基因别名: ASD2; Gata-4; GATA4; TACHD; TOF; VSD1

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UniProt ID: (Human) P43694, (Mouse) Q08369

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Entrez Gene ID: (Human) 2626, (Mouse) 14463

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RNA polymerase II regulatory region sequence-specific DNA binding transcription factor activity, RNA polymerase II transcription factor binding RNA polymerase II transcription factor binding enhancer sequence-specific DNA binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding DNA binding chromatin binding transcription factor activity, sequence-specific DNA binding transcription coactivator activity protein binding transcription factor binding zinc ion binding protein kinase binding activating transcription factor binding sequence-specific DNA binding transcription regulatory region DNA binding NFAT protein binding co-SMAD binding RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding metal ion binding DNA-binding transcription factor

heart looping endocardial cushion development cardiac ventricle morphogenesis cardiac right ventricle morphogenesis ventricular septum development atrial septum primum morphogenesis atrial septum secundum morphogenesis regulation of transcription, DNA-templated transcription from RNA polymerase II promoter cell-cell signaling endoderm development blood coagulation male gonad development response to mechanical stimulus negative regulation of autophagy positive regulation of vascular endothelial growth factor production positive regulation of BMP signaling pathway response to vitamin A embryonic heart tube anterior/posterior pattern specification response to drug cell fate commitment positive regulation of angiogenesis positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter cell development embryonic foregut morphogenesis anatomical structure formation involved in morphogenesis positive regulation of cardioblast differentiation transdifferentiation atrial septum morphogenesis intestinal epithelial cell differentiation cell growth involved in cardiac muscle cell development cellular response to glucose stimulus regulation of cardiac muscle cell contraction in utero embryonic development gastrulation with mouth forming second endoderm formation heart morphogenesis outflow tract morphogenesis atrioventricular valve morphogenesis atrioventricular valve formation mitral valve formation tricuspid valve formation ventricular cardiac muscle tissue development cardiac septum development transcription, DNA-templated regulation of transcription from RNA polymerase II promoter spermatogenesis heart development regulation of gene expression cardiac muscle hypertrophy in response to stress signal transduction involved in regulation of gene expression BMP signaling pathway response to retinoic acid embryonic heart tube development tube morphogenesis skeletal muscle cell differentiation atrioventricular canal development response to estrogen embryonic digestive tract morphogenesis embryonic morphogenesis cardiac muscle tissue development cardiac muscle cell differentiation Sertoli cell differentiation regulation of cardiac muscle cell proliferation positive regulation of cardiac muscle cell proliferation canonical Wnt signaling pathway SMAD protein signal transduction lung morphogenesis lung lobe formation diaphragm morphogenesis vasculogenesis involved in coronary vascular morphogenesis cellular response to gonadotropin stimulus cellular response to follicle-stimulating hormone stimulus epithelial cell fate commitment seminiferous tubule development