Invitrogen

GLP7 Polyclonal Antibody

问题与答案

问题与答案

问题与答案

抗体检测数据 (2)

GLP7 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

GLP7 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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GLP7 Antibody (PA5-119695) in IHC (P)

Immunohistochemistry (Paraffin) analysis of paraffin-embedded A.thaliana using GLP7 Polyclonal Antibody (Product # PA5-119695). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes and sodium citrate buffer (pH 6) for 20 mins. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with th... View More

GLP7 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

GLP7 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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GLP7 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry (Paraffin) analysis of paraffin-embedded A.thaliana using GLP7 Polyclonal Antibody (Product # PA5-119695). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes and sodium citrate buffer (pH 6) for 20 mins. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the GLP7 antibody at a dilution of 1:50 dilution for 10 hours at 4°C and detected using Alexa Fluor 488 conjugate-Goat anti-Rabbit IgG (H+L) Secondary Antibody at a dilution of 1:500 for 1 hour at room temperature.

GLP7 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry (Paraffin) analysis of paraffin-embedded A.thaliana tissue using GLP7 Polyclonal Antibody (Product # PA5-119695). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the GLP7 antibody at a dilution of 1:200 for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

产品信息

PA5-119695

应用

建议稀释比

已发表文章

免疫组化（石蜡） (IHC (P))

1:50-1:100

-

产品规格

种属反应

Plant

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant protein within A. thaliana AtGLP7 aa 10-217

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

TBS, pH 7.4, with 50% glycerol, 0.2% BSA

内含物

0.05% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

Positive Control: A. thaliana tissue.

Subcellular Location: Apoplast.

靶标信息

May play a role in plant defense. Probably has no oxalate oxidase activity even if the active site is conserved.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: Germin-like protein subfamily 1 member 1

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基因别名: AT1G10460; germin-like protein 7; GLP7; T10O24.6; T10O24.7

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UniProt ID: (Arabidopsis) P92998

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Entrez Gene ID: (Arabidopsis) 837586

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manganese ion binding nutrient reservoir activity

response to stress