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Invitrogen
GPNMB Recombinant Rat Monoclonal Antibody (CTSREVL)
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GPNMB Antibody (740113T) in ICC/IF
Immunofluorescence analysis of GPNMB was performed using GPNMB Recombinant Rat Monoclonal Antibody (CTSREVL) (Product # 740113T, 1:200) on 70-80% confluent B16-F10 and EL4 cells lines. The cell lines were fixed with 4% paraformaldehyde for 10 minutes and then blocked with 2% BSA for 1 hour at room temperature. The cells were stained with GPNMB Recombinant Rat Monoclonal Antibody (CTSREVL) in 0.1% BSA, incubated at 4 degree Celsius overnight, followed by labelling with Donkey anti-Rat IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alex... View More
产品信息
740113T
产品规格
种属反应
Mouse
宿主/亚型
Rat
/ IgG2a, kappa
Expression System
Expi293
分类
Recombinant Monoclonal
类型
Antibody
克隆号
CTSREVL
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein A/G
保存液
PBS, pH 7.4
内含物
0.09% sodium azide
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Wet ice
产品详细信息
Recombinant rat monoclonal antibodies are produced using in vitro expression systems. Recombinant antibodies are produced using specific genes that code for the desired antibodies. These genes are cloned into an expression vector and expressed in vitro. The advantages of recombinant antibodies include better specificity and lot-to-lot consistency.
It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
靶标信息
The protein encoded by this gene is a type I transmembrane glycoprotein which shows homology to the pMEL17 precursor, a melanocyte-specific protein. GPNMB shows expression in the lowly metastatic human melanoma cell lines and xenografts but does not show expression in the highly metastatic cell lines. GPNMB may be involved in growth delay and reduction of metastatic potential. Two transcript variants encoding different isoforms have been found for this gene.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: DC-HIL; dendritic cell-associated heparin sulfate proteoglycan-dependent integrin ligand; Dendritic cell-associated transmembrane protein; iris pigment dispersion; Osteoactivin; Transmembrane glycoprotein NMB
基因别名: DC-HIL; Dchil; Gpnmb; Hgfin; ipd; Nmb
UniProt ID: (Mouse) Q99P91
Entrez Gene ID: (Mouse) 93695
