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Invitrogen
GPNMB Recombinant Rat Monoclonal Antibody (CTSREVL), Alexa Fluor™ Plus 555
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GPNMB Antibody (740113TP555) in ICC/IF
Immunofluorescence analysis of GPNMB was performed using GPNMB Recombinant Rat Monoclonal Antibody (CTSREVL), Alexa Fluor™ Plus 555 (Product # 740113TP555) on 70-80% confluent B16-F10 and EL4 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes and then blocked with 2% BSA for 1 hour at room temperature. The cells were stained with GPNMB Recombinant Rat Monoclonal Antibody (CTSREVL), Alexa Fluor™ Plus 555 (Product # 740113TP555, 1:100) in 0.1% BSA, incubated at 4 degree Celsius overnight, Panel a) shows representative image ... View More
产品信息
740113TP555
产品规格
种属反应
Mouse
宿主/亚型
Rat
/ IgG2a, kappa
Expression System
Expi293
分类
Recombinant Monoclonal
类型
Antibody
克隆号
CTSREVL
偶联物
激发/发射光谱
558/572 nm
查看光谱
形式
Liquid
浓度
1 mg/mL
纯化类型
Affinity chromatography
保存液
proprietary buffer, pH 6.3-7.3
内含物
0.008% Bromonitrodioxane, 0.008% Methylisothiazolone
保存条件
4°C, store in dark, DO NOT FREEZE!
运输条件
Wet ice
产品详细信息
Alexa Fluor™ Plus recombinant antibodies are conjugated using new, proprietary dye chemistry so you can generate stunning data. Alexa Fluor™ Plus antibodies represent an advancement in fluorescent conjugate technology. Alexa Fluor™ Plus antibodies provide brighter signal compared to leading Alexa Fluor™ antibodies, providing you with better signal-to-noise for your critical experiments. These antibodies show better specificity and lot-to-lot consistency as these are recombinant antibodies, generated by cloning specific genes for the desired antibodies into an expression vector and expressed in vitro.
Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment. This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining.
It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 553 nm; Emission: 568 nm; Laser: Yellow Laser
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
The protein encoded by this gene is a type I transmembrane glycoprotein which shows homology to the pMEL17 precursor, a melanocyte-specific protein. GPNMB shows expression in the lowly metastatic human melanoma cell lines and xenografts but does not show expression in the highly metastatic cell lines. GPNMB may be involved in growth delay and reduction of metastatic potential. Two transcript variants encoding different isoforms have been found for this gene.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: DC-HIL; dendritic cell-associated heparin sulfate proteoglycan-dependent integrin ligand; Dendritic cell-associated transmembrane protein; iris pigment dispersion; Osteoactivin; Transmembrane glycoprotein NMB
基因别名: DC-HIL; Dchil; Gpnmb; Hgfin; ipd; Nmb
UniProt ID: (Mouse) Q99P91
Entrez Gene ID: (Mouse) 93695
