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Invitrogen
GPNMB Recombinant Rabbit Monoclonal Antibody (PSH0-82)
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GPNMB Antibody (MA5-49360) in ICC/IF
Immunofluorescence analysis of GPNMB using L929 cells. The cells were fixed 4% paraformaldehyde for 10 minutes, permeabilized with 0.05% Triton™ X-100 in PBS for 20 minutes, and blocked with 2% negative goat serum for 30 minutes at room temperature. The cells were labeled with GPNMB Rabbit Recombinant Monoclonal Antibody (PSH0-82) (Product # MA5-49360) at 1:100 dilution in 2% negative goat serum overnight at 4°C and then with iFluor™ 488 Goat Anti-Rabbit IgG H&L secondary antibody (1:1,000 dilution) for 1 hour at room temperature (green). ... View More
产品信息
MA5-49360
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
Expression System
Expi293
分类
Recombinant Monoclonal
类型
Antibody
克隆号
PSH0-82
抗原
Recombinant protein within human gpnmb aa 1-498 / 572.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein A
保存液
PBS, pH 7.4, with 0.1% BSA, 40% glycerol
内含物
0.05% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Sequence Similarities: 98% Mouse/Rat.
Tissue Specificity: Widely expressed, but very low expression, if any, in the brain (PubMed:12609765, PubMed:16609006). Expressed in the epidermis with higher levels in melanocytes compared with keratinocytes and Langerhans cells (at protein level) (PubMed:29336782). Expressed in peripheral blood, but not bone marrow mononuclear cells (PubMed:12609765). Expressed in tissue macrophages, including liver Kuppfer cells and lung alveolar macrophages, in podocytes and in some cells of the ciliary body of the eye (at protein level) (PubMed:16489096). May be overexpressed in various cancers, including melanoma and glioblastoma multiforme (PubMed:7814155, PubMed:16489096, PubMed:16609006).
Positive Control: L929 cell lysate, A375 cell lysate, U-937 cell lysate, rat heart tissue lysate, B16F1 cell lysates, human lung tissue, L929.
Subcellular Location: Cell membrane, Melanosome membrane, Early endosome membrane.
Predicted band size: 64 kDa.
靶标信息
The protein encoded by this gene is a type I transmembrane glycoprotein which shows homology to the pMEL17 precursor, a melanocyte-specific protein. GPNMB shows expression in the lowly metastatic human melanoma cell lines and xenografts but does not show expression in the highly metastatic cell lines. GPNMB may be involved in growth delay and reduction of metastatic potential. Two transcript variants encoding different isoforms have been found for this gene.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: DC-HIL; dendritic cell-associated heparin sulfate proteoglycan-dependent integrin ligand; Dendritic cell-associated transmembrane protein; glycoprotein (transmembrane) nmb; glycoprotein nmb-like protein; glycoprotein nonmetastatic melanoma protein B; hematopoietic growth factor inducible neurokinin-1; Hematopoietic growth factor inducible neurokinin-1 type; iris pigment dispersion; Osteoactivin; transmembrane glycoprotein HGFIN; Transmembrane glycoprotein NMB
基因别名: DC-HIL; Dchil; GPNMB; HGFIN; ipd; NMB; UNQ1725/PRO9925
UniProt ID: (Human) Q14956, (Mouse) Q99P91
Entrez Gene ID: (Human) 10457, (Rat) 113955, (Mouse) 93695
