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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • GPR40 Antibodies

          Invitrogen

          GPR40 Polyclonal Antibody

          查看其他11个GPR40抗体

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          说明书
          实验方案
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          Cite GPR40 Polyclonal Antibody

          • 抗体检测数据 (1)
          GPR40 Antibody in Western Blot (WB)
          Group 53 Created with Sketch.
          GPR40 Antibody in Western Blot (WB)
          Group 53 Created with Sketch.

          图: 1 / 1

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          GPR40 Antibody (PA5-95511) in WB

          Western blot analysis of GPR40 in Lane 1: human PANC-1 whole cell lysates. Electrophoresis was performed with 5-20% SDS-PAGE gel (70V, Stacking gel; 90V Resolving gel, Time: 2-3 hours), transferred to a nitrocellulose membrane and blocked using 5% Non-fat Milk/TBS (1.5 hrs at room temperature). Samples were incubated with GPR40 polyclonal antibody (Product # PA5-95511) using a 0.5 µg/mL dilution, followed by a goat anti-rabbit IgG-HRP at a dilution of 1:10,000, and developed with enhanced chemiluminescence (ECL). {{ $ctrl.currentElement.advancedVerification.fullName }} 验证信息 View more
          已发表图片由___提供 benchsci-logo
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          GPR40 Antibody in Western Blot (WB)
          GPR40 Polyclonal Antibody

          产品信息

          PA5-95511

          应用
          建议稀释比
          已发表文章

          免疫印迹 (WB)

          0.1-0.5 µg/mL
          -
          产品规格

          种属反应

          Human, Mouse, Rat

          宿主/亚型

          Rabbit / IgG

          分类

          Polyclonal

          类型

          Antibody

          抗原

          A synthetic peptide corresponding to a sequence of human GPCR GPR40 (YVGCLRALARSGLTHRRKLRAAWVA).
          查看免疫原

          偶联物

          Unconjugated Unconjugated Unconjugated

          形式

          Lyophilized

          浓度

          500 µg/mL

          纯化类型

          Affinity chromatography

          保存液

          PBS with 4mg trehalose

          内含物

          0.05mg sodium azide

          保存条件

          -20°C

          运输条件

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2807313

          产品详细信息

          Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

          Positive Control - WB: human PANC-1 whole cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

          靶标信息

          This gene encodes a member of the GP40 family of G protein-coupled receptors that are clustered together on chromosome 19. The encoded protein is a receptor for medium and long chain free fatty acids and may be involved in the metabolic regulation of insulin secretion. Polymorphisms in this gene may be associated with type 2 diabetes.

          仅用于科研。不用于诊断过程。未经明确授权不得转售。

          篇参考文献 (0)

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          引用该产品

          生物信息学

          蛋白别名: FFAR; Free fatty acid receptor 1; G protein-coupled receptor 40; G protein-coupled receptor GPR40; G-protein coupled receptor 40; HGNC:4498

          查看更多 收起

          基因别名: FFA1R; FFAR1; GPCR40; GPR40

          查看更多 收起

          UniProt ID: (Human) O14842, (Rat) Q8K3T4, (Mouse) Q76JU9

          查看更多 收起

          Entrez Gene ID: (Human) 2864, (Rat) 266607, (Mouse) 233081

          查看更多 收起

          功能
          G-protein coupled receptor activity guanyl-nucleotide exchange factor activity lipid binding bioactive lipid receptor activity fatty acid binding signal transducer activity
          参与通路
          G-protein coupled receptor signaling pathway positive regulation of calcium ion transport into cytosol insulin secretion positive regulation of insulin secretion glucose homeostasis positive regulation of calcium ion transport response to fatty acid signal transduction positive regulation of GTPase activity
          It has to be done as per old AB suggested Products section.
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          性能保障

          只要您发现Invitrogen抗体在实验中的表现与网站或说明书的所述内容不符,您即可享受免费退换货服务。*

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