Invitrogen

Galectin 3 Monoclonal Antibody (A3A12)

Name: Galectin 3 Monoclonal Antibody (A3A12)
Brand: Invitrogen
SKU: MA1-940

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15 篇参考文献

查看其他63个Galectin 3抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

Galectin 3 Antibody in Immunocytochemistry (ICC/IF)

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Galectin 3 Antibody (MA1-940) in ICC/IF

Immunofluorescent analysis of Galectin 3 (green) showing staining in the cytoplasm and nucleus of NIH-3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Galectin 3 monoclonal antibody (Product # MA1-940) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 °C i... View More

Galectin 3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Galectin 3 Antibody in Immunohistochemistry (IHC)

Galectin 3 Antibody in Western Blot (WB)

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Immunogen image

Immunogen Image Galectin 3 Monoclonal Antibody (A3A12)

产品信息

MA1-940

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

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免疫组化 (IHC)

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免疫组化（石蜡） (IHC (P))

1:20-1:200

免疫组化（冰冻） (IHC (F))

6 µg/mL

免疫细胞化学 (ICC/IF)

1:20-1:200

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酶联免疫吸附实验 (ELISA)

Assay-dependent

产品规格

种属反应

Human, Mouse, Rat

已发表种属

C. elegans, Human, Mouse, Rat

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

A3A12

抗原

Full length recombinant human galectin-3.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

purified

保存液

PBS

内含物

0.05% sodium azide

保存条件

-20°C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

AB_2136775

产品详细信息

MA1-940 detects galectin-3 from human, mouse and rat tissues.

MA1-940 has been successfully used in Western blot, immunohistochemistry, immunofluorescence, immunocytochemistry and ELISA procedures. By Western blot, this antibody detects an ~30 kDa protein representing galectin-3 from Jurkat cells transfected with human galectin-3. Immunohistochemical staining of galectin-3 in rat olfactory bulb with MA1-940 yields a pattern consistent with nuclear and plasma membrane staining. This antibody is not suitable for mouse NIH-3T3 in Western blot analysis. This antibody also detects a non-specific band in Hela cell lysates at ~21 kDa.

MA1-940 antigen is full length recombinant human galectin-3. The epitope for this antibody has been mapped to the first 58 amino acids of galectin-3.

靶标信息

Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis. In the nucleus: acts as a pre-mRNA splicing factor. Involved in acute inflammatory responses including neutrophil activation and adhesion, chemoattraction of monocytes macrophages, opsonization of apoptotic neutrophils, and activation of mast cells.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: 35 kDa lectin; advanced glycation end-product receptor 3; Carbohydrate-binding protein 35; CBP 35; CBP-30; epsilon BP; Gal-3; Galactose-specific lectin 3; Galactoside-binding protein; GALBP; Galectin-3; Galectin3; IgE binding protein; IgE-binding protein; L-29; L-31; L-34 galactoside-binding lectin; Laminin-binding protein; lectin galactoside-binding soluble 3 protein; Lectin L-29; lectin, galactose binding, soluble 3; lectin, galactoside-binding, soluble, 3; MAC-2 antigen

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基因别名: AGE-R3; CBP30; CBP35; gal-3; GAL3; GALBP; GALIG; GBP; L-34; L31; LGALS2; LGALS3; Mac-2; MAC2

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UniProt ID: (Human) P17931, (Rat) P08699

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Entrez Gene ID: (Human) 3958, (Mouse) 16854, (Rat) 83781

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