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Gata-4 Antibody (14-9980-82) in WB
Western blot analysis was performed on tissue extracts (30 µg lysate) of Mouse Heart (Lane 1), Mouse Ovary (Lane 2) and Mouse Brain (Lane 3). The blot was probed with Anti-GATA-4 Rat Monoclonal Antibody (Product # 14-9980-82, 1:1000 dilution) and detected by chemiluminescence using F(ab"e)2-Rabbit anti-Rat IgG (H+L) Secondary Antibody, HRP (Product # PA1-29927, 0.25 ug/ml, 1:4000 dilution). A 50 kDa band corresponding to GATA-4 was detected across the tissues tested. Additional band around 25kDa from circulating IgGs was observed in Mo... View More
产品信息
14-9980-82
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse
已发表种属
C. elegans,
Human,
Mouse,
Rat
宿主/亚型
Rat
/ IgG2a, kappa
分类
Monoclonal
类型
Antibody
克隆号
eBioEvan
偶联物
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2
内含物
0.09% sodium azide
保存条件
4°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Description: The eBioEvan monoclonal antibody reacts with mouse and human Gata-4. In a western blot of C57Bl/6 and BALB/c mouse heart lysates, a single band is present, suggesting that this antibody does not cross-react with Gata-5 or Gata-6, which are also expressed in the heart, but with different molecular weights. The eBioEvan monoclonal antibody has been successfully used in flow cytometry on HEK 293T cells transiently transfected with either mouse Gata-4 or human Gata-4.
Gata-4 is a member of the GATA family of transcription factors, which are characterized by a conserved DNA binding domain containing two zinc fingers. The mouse Gata-4 gene encodes a protein of 48 kDa. Studies show that Gata-4 plays an important role in controlling cardiomyocyte differentiation, proliferation and survival. Gata-4 is expressed during fetal development, as well as in the adult mouse heart. It also appears to modulate gene expression in the gut, liver, and gonads.
Applications Reported: This eBioEvan antibody has been reported for use in immunoblotting (WB) and immunohistochemistry.
Applications Tested: This eBioEvan antibody has been tested by western blot analysis of mouse heart lysates. As a starting dilution, it is recommended to use this purified antibody at 2 µg/mL for western blot analysis. The eBioEvan antibody has been tested on formalin-fixed paraffin embedded human tissue using low pH antigen retrieval and can be used at less than or equal to 5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
GATA4 is a member of the GATA family of zinc-finger transcription factors. Members of this family recognize the GATA motif which is present in the promoters of many genes. This protein is thought to regulate genes involved in embryogenesis and in myocardial differentiation and function. Mutations in the gene encoding GATA4 have been associated with cardiac septal defects.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: GAT4; GATA-binding factor 4; GATA-binding protein 4; MGC126629; Transcription factor GATA-4
基因别名: ASD2; Gata-4; GATA4; TACHD; TOF; VSD1
UniProt ID: (Human) P43694, (Mouse) Q08369
Entrez Gene ID: (Human) 2626, (Mouse) 14463
RNA polymerase II regulatory region sequence-specific DNA binding
transcription factor activity, RNA polymerase II transcription factor binding
RNA polymerase II transcription factor binding
enhancer sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
DNA binding
chromatin binding
transcription factor activity, sequence-specific DNA binding
transcription coactivator activity
protein binding
transcription factor binding
zinc ion binding
protein kinase binding
activating transcription factor binding
sequence-specific DNA binding
transcription regulatory region DNA binding
NFAT protein binding
co-SMAD binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding
metal ion binding
DNA-binding transcription factor
heart looping
endocardial cushion development
cardiac ventricle morphogenesis
cardiac right ventricle morphogenesis
ventricular septum development
atrial septum primum morphogenesis
atrial septum secundum morphogenesis
regulation of transcription, DNA-templated
transcription from RNA polymerase II promoter
cell-cell signaling
endoderm development
blood coagulation
male gonad development
response to mechanical stimulus
negative regulation of autophagy
positive regulation of vascular endothelial growth factor production
positive regulation of BMP signaling pathway
response to vitamin A
embryonic heart tube anterior/posterior pattern specification
response to drug
cell fate commitment
positive regulation of angiogenesis
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
cell development
embryonic foregut morphogenesis
anatomical structure formation involved in morphogenesis
positive regulation of cardioblast differentiation
transdifferentiation
atrial septum morphogenesis
intestinal epithelial cell differentiation
cell growth involved in cardiac muscle cell development
cellular response to glucose stimulus
regulation of cardiac muscle cell contraction
in utero embryonic development
gastrulation with mouth forming second
endoderm formation
heart morphogenesis
outflow tract morphogenesis
atrioventricular valve morphogenesis
atrioventricular valve formation
mitral valve formation
tricuspid valve formation
ventricular cardiac muscle tissue development
cardiac septum development
transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
spermatogenesis
heart development
regulation of gene expression
cardiac muscle hypertrophy in response to stress
signal transduction involved in regulation of gene expression
BMP signaling pathway
response to retinoic acid
embryonic heart tube development
tube morphogenesis
skeletal muscle cell differentiation
atrioventricular canal development
response to estrogen
embryonic digestive tract morphogenesis
embryonic morphogenesis
cardiac muscle tissue development
cardiac muscle cell differentiation
Sertoli cell differentiation
regulation of cardiac muscle cell proliferation
positive regulation of cardiac muscle cell proliferation
canonical Wnt signaling pathway
SMAD protein signal transduction
lung morphogenesis
lung lobe formation
diaphragm morphogenesis
vasculogenesis involved in coronary vascular morphogenesis
cellular response to gonadotropin stimulus
cellular response to follicle-stimulating hormone stimulus
epithelial cell fate commitment
seminiferous tubule development
