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Invitrogen
Glucocorticoid Receptor Monoclonal Antibody (BuGR2)
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Glucocorticoid Receptor Antibody (MA1-510) in ICC/IF
Immunofluorescent analysis of Glucocorticoid Receptor using Glucocorticoid Receptor Monoclonal Antibody (BuGR2) (Product # MA1-510) shows staining in A549 Cells. Glucocorticoid Receptor (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody ... View More
产品信息
MA1-510
应用
建议稀释比
已发表文章
产品规格
种属反应
Guinea pig,
Human,
Mouse,
Sheep,
Rabbit,
Rat,
Yeast
已发表种属
C. elegans,
Human,
Mouse,
Non-human primate,
Rabbit,
Rat,
Sheep,
Xenopus,
Yeast
宿主/亚型
Mouse
/ IgG2a
分类
Monoclonal
类型
Antibody
克隆号
BuGR2
抗原
Partially purified rat GR.
偶联物
形式
Lyophilized
浓度
1 mg/mL
纯化类型
Protein A
保存液
PBS, pH 7.2
内含物
0.05% sodium azide
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
MA1-510 detects glucocorticoid receptor (GR) from human, mouse, rat, guinea pig, rabbit, sheep and yeast samples. This antibody does not react with primate, avian or amphibian GR.
MA1-510 has been successfully used in Western blot, immunofluorescence, immunocytochemistry, flow cytometry, immunohistochemistry, immunoprecipitation, and gel shift procedures. By Western blot, this antibody detects a 97 kDa protein representing GR in L929 cell extract. Immunocytochemical staining of GR in L929 cells with MA1-510 results in staining of both the cytoplasm and nucleus, even in the presence of hormone. Using enzymatic digestion analysis, MA1-510 reacts with the undigested 97 kDa GR, a 17 kDa DNA-binding trypsin fragment, and a 45 kDa steroid- and DNA-binding chymotrypsin fragment.
The MA1-510 immunogen is partially purified rat GR.
Reconstitute with 100 µL distilled water.
靶标信息
Glucocorticoid Receptor (NR3C1) is a receptor for glucocorticoids that can act as both a transcription factor and as a regulator of other transcription factors. Glucocorticoid Receptor can also be found in heteromeric cytoplasmic complexes along with heat shock factors and immunophilins. The protein is typically found in the cytoplasm until it binds a ligand, which induces transport into the nucleus. Glucocorticoid Receptor is expressed in the heart, detected in left and right atria, left and right ventricles, aorta, apex, intraventricular septum, and atrioventricular node as well as whole adult and fetal heart. Alternate splicing, the use of at least three different promoters, and alternate translation initiation sites result in several transcript variants encoding the same protein or different isoforms, but the full-length nature of some variants has not been determined. Mutations in the Glucocorticoid Receptor gene are a cause of glucocorticoid resistance, or cortisol, resistance. Studies have shown that glucocorticoid receptor (GR) must be associated with a complex of chaperone proteins for ligand activation. GR binds to known steroids such as dexamethasone with nanomolar affinity
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: cytosolic/nuclear receptor; glucocorticoid nuclear receptor variant 1; Glucocorticoid receptor; glucocorticoid receptor 1; glucocorticoid receptor alpha; GR; gr alpha; GR Kit; Nuclear receptor subfamily 3 group C member 1; nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor); OTTHUMP00000222854; OTTHUMP00000222858
基因别名: GCCR; GCR; GCRST; GR; GRL; Grl-1; Grl1; NR3C1
UniProt ID: (Rabbit) P59667, (Human) P04150, (Mouse) P06537, (Guinea pig) P49115, (Rat) P06536
Entrez Gene ID: (Sheep) 443412, (Rabbit) 100008890, (Human) 2908, (Mouse) 14815, (Guinea pig) 100135583, (Rat) 24413
RNA polymerase II core promoter proximal region sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
transcription factor activity, sequence-specific DNA binding
RNA binding
glucocorticoid receptor activity
steroid binding
protein binding
zinc ion binding
protein complex binding
glucocorticoid-activated RNA polymerase II transcription factor binding transcription factor activity
steroid hormone binding
RNA polymerase II regulatory region DNA binding
DNA binding
chromatin binding
double-stranded DNA binding
steroid hormone receptor activity
transcription coactivator activity
transcription factor binding
lipid binding
Hsp70 protein binding
receptor tyrosine kinase binding
heat shock protein binding
hormone binding
protein homodimerization activity
sequence-specific DNA binding
metal ion binding
protein heterodimerization activity
protein dimerization activity
Hsp90 protein binding
regulation of gluconeogenesis
chromatin remodeling
transcription, DNA-templated
regulation of transcription, DNA-templated
transcription from RNA polymerase II promoter
glucocorticoid metabolic process
regulation of glucose metabolic process
positive regulation of glutamate secretion
chromatin modification
adrenal gland development
negative regulation of synaptic plasticity
regulation of glucocorticoid biosynthetic process
regulation of cell proliferation
maternal behavior
glucocorticoid receptor signaling pathway
negative regulation of vascular permeability
steroid hormone mediated signaling pathway
glucocorticoid mediated signaling pathway
positive regulation of neuron apoptotic process
positive regulation of transcription from RNA polymerase II promoter
response to arsenic-containing substance
chromatin-mediated maintenance of transcription
mammary gland duct morphogenesis
positive regulation of dendritic spine development
positive regulation of cell growth involved in cardiac muscle cell development
cellular response to steroid hormone stimulus
cellular response to dexamethasone stimulus
negative regulation of glucocorticoid mediated signaling pathway
positive regulation of glucocorticoid receptor signaling pathway
brain development
aging
circadian rhythm
androgen metabolic process
response to radiation
response to activity
response to inactivity
muscle atrophy
lung development
response to insulin
response to mercury ion
response to corticosterone
response to calcium ion
response to electrical stimulus
cellular response to magnesium ion
response to dexamethasone
transcription initiation from RNA polymerase II promoter
apoptotic process
chromosome segregation
mitotic nuclear division
signal transduction
covalent chromatin modification
cell division
