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Invitrogen
F(ab')2-Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, HRP
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Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody (A10547) in WB
Chemiluminescent western blot was performed using F(ab)2-Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, HRP (Product # A10547). Membrane enriched extracts of THP1 (Lane 1, 2, 3, 4), MCF7 (Lane 5, 6, 7, 8) and HeLa (Lane 9) were electrophoresed usingNuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were transferred onto anitrocellulose membrane (Product # IB23001) byi... View More
产品信息
A10547
应用
建议稀释比
免疫印迹 (WB)
1:5,000-1:10,000
免疫组化 (IHC)
1:500-1:2,000
酶联免疫吸附实验 (ELISA)
1:500-1:2,000
产品规格
种属反应
Rabbit
宿主/亚型
Goat
/ IgG
分类
Polyclonal
类型
Secondary Antibody
抗原
Gamma Immunoglobins Heavy and Light chains
偶联物
形式
Lyophilized
纯化类型
purified
保存液
PBS
内含物
no preservative
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
靶标
IgG
交叉吸附
Against pooled human serum, mouse serum, mouse plasmacytoma/hybridoma proteins and purified human paraproteins
抗体形式
F(ab')2 Fragment
产品详细信息
Storage and reconstitution: The formulation of this antibody was updated from PBS with 0.02% thimerosal to PBS without thimerosal on 31/Mar/2024, any lots produced after this date will not contain thimerosal. Lot numbers greater than 2855945 will not have thimerosal.
Store product at -20°C until opened. Reconstitute using 0.5 mL PBS, pH 7.2, to yield a 1 mg/mL stock solution. After reconstitution in buffer without a stabilizer like thimerosal, the product is stable for approximately three months at 4°C. For prolonged storage after reconstitution in buffers without a stabilizer, add glycerol to a final concentration of 50% (v/v), aliquot, and store at -20°C. The antibody will be stable for up to one year stored in this manner.
Alternatively, if the product is reconstituted in a buffer containing a preservative, for example, in PBS, pH 7.2, containing 0.02% thimerosal, the product will be stable for up to one year when stored at 4°C.
靶标信息
Anti-Rabbit secondary antibodies are affinity-purified antibodies with well-characterized specificity for rabbit immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
