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Bethyl Laboratories

HIF1-alpha Polyclonal Antibody

View all (67) HIF1A antibodies

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HIF1-alpha Polyclonal Antibody

Product Details

A300-286A

Applications
Tested Dilution
Publications

Western Blot (WB)

1:2,000-1:10,000
-

Immunohistochemistry (IHC)

1:100-1:500
-

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:500
-

Immunocytochemistry (ICC/IF)

1:100-1:500
-

Immunoprecipitation (IP)

2-10 µg/mg lysate
-
Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Region between residues 775 and the C-terminus (residue 826) of human hypoxia-inducible factor 1, alpha subunit.
3D Epitope / Immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.20 mg/mL

Amount

20 µg

Purification

Antigen affinity chromatography

Storage buffer

TBS, pH 7.0 to 8.0, with 0.1% BSA

Contains

0.09% sodium azide

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

The recommended shelf life for this product is 1 year from date of receipt.

Based on 100% sequence identity, this antibody is predicted to react with Rat, Chicken and Bovine

Target Information

HIF1-alpha (HIF1A) is a subunit of HIF1, which is a transcription factor found in mammalian cells cultured under reduced oxygen tension. HIF-1 is a heterodimer consisting of an alpha and beta subunit, both belonging to the basic-helix-loop-helix Per-aryl hydrocarbon receptor nuclear translocator-Sim (PAS) family of transcription factors. HIF1 functions as a transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions, HIF-1 activates the transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, HILPDA, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. HIF1-alpha regulates hypoxia-mediated apoptosis, cell proliferation and tumor angiogenesis. Hypoxia which induces p53 protein accumulation, directly interacts with HIF1-alpha and reduces hypoxia-induced expression of HIF1-alpha by promoting MDM2-mediated ubiquitination and proteasomal degradation under hypoxic conditions. Recent studies suggest that induction of NOX4 by HIF1-alpha contributes to maintain ROS levels after hypoxia and hypoxia-induced proliferation. In humans, it is located on the q arm of chromosome 14. The C-terminal of HIF1A binds to p300. p300/CBP-HIF complexes participate in the induction of hypoxia-responsive genes, including VEGF. Hypoxia contributes significantly to the pathophysiology of major categories of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease and chronic obstructive pulmonary disease.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: ARNT-interacting protein; ARNT2; hif 1; hif 1a; HIF-1-alpha; HIF1-alpha; Hypoxia-inducible factor 1-alpha

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Gene Aliases: AA959795; bHLHe78; Hif1a; HIF1alpha; MOP1

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UniProt ID: (Mouse) Q61221

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Entrez Gene ID: (Mouse) 15251

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Function(s)
RNA polymerase II transcription factor activity, sequence-specific DNA binding transcription factor activity, transcription factor binding transcription factor activity, RNA polymerase II transcription factor binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding DNA binding transcription factor activity, sequence-specific DNA binding transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding signal transducer activity protein binding transcription factor binding enzyme binding protein kinase binding ubiquitin protein ligase binding protein complex binding histone acetyltransferase binding nuclear hormone receptor binding histone deacetylase binding sequence-specific DNA binding protein heterodimerization activity protein dimerization activity Hsp90 protein binding basic helix-loop-helix transcription factor
Process(es)
angiogenesis blood vessel development response to hypoxia neural crest cell migration epithelial to mesenchymal transition embryonic placenta development B-1 B cell homeostasis vasculature development heart looping positive regulation of neuroblast proliferation connective tissue replacement involved in inflammatory response wound healing outflow tract morphogenesis cardiac ventricle morphogenesis lactate metabolic process regulation of glycolytic process transcription, DNA-templated regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase II promoter cellular iron ion homeostasis signal transduction lactation positive regulation of cell proliferation visual learning regulation of gene expression positive regulation of autophagy vascular endothelial growth factor production positive regulation of vascular endothelial growth factor production positive regulation of gene expression positive regulation of epithelial cell migration positive regulation of receptor biosynthetic process response to muscle activity axonal transport of mitochondrion neural fold elevation formation cerebral cortex development cell differentiation negative regulation of bone mineralization positive regulation of vascular endothelial growth factor receptor signaling pathway negative regulation of TOR signaling oxygen homeostasis regulation of transforming growth factor beta2 production collagen metabolic process negative regulation of transcription elongation from RNA polymerase II promoter embryonic hemopoiesis positive regulation of insulin secretion involved in cellular response to glucose stimulus regulation of cell proliferation hemoglobin biosynthetic process glucose homeostasis positive regulation of apoptotic process negative regulation of apoptotic process negative regulation of neuron apoptotic process regulation of transcription from RNA polymerase II promoter in response to oxidative stress positive regulation of erythrocyte differentiation positive regulation of transcription, DNA-templated negative regulation of vasoconstriction negative regulation of growth positive regulation of transcription from RNA polymerase II promoter muscle cell cellular homeostasis positive regulation of hormone biosynthetic process blood vessel morphogenesis digestive tract morphogenesis positive regulation of smooth muscle cell proliferation regulation of catalytic activity cartilage development elastin metabolic process intestinal epithelial cell maturation response to fungicide epithelial cell differentiation involved in mammary gland alveolus development retina vasculature development in camera-type eye positive regulation of transcription from RNA polymerase II promoter in response to hypoxia regulation of thymocyte apoptotic process negative regulation of thymocyte apoptotic process cellular response to hypoxia dopaminergic neuron differentiation hypoxia-inducible factor-1alpha signaling pathway negative regulation of oxidative stress-induced neuron intrinsic apoptotic signaling pathway positive regulation of mitophagy regulation of aerobic respiration negative regulation of reactive oxygen species metabolic process negative regulation of mesenchymal cell apoptotic process
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