Invitrogen

HLA-DRB4 Polyclonal Antibody

Name: HLA-DRB4 Polyclonal Antibody
Brand: Invitrogen
SKU: PA5-87621

查看其他6个HLA-DRB4抗体

说明书

证书

实验方案

问题与答案

技术支持

说明书

证书

实验方案

问题与答案

技术支持

说明书

证书

实验方案

问题与答案

技术支持

Additional Information:

抗体检测数据 (1)

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HLA-DRB4 Antibody (PA5-87621) in WB

Western blot analysis of HLA-DRB4 in extracts of various cell lines. Samples were incubated with HLA-DRB4 Polyclonal antibody (Product # PA5-87621) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

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Immunogen image

Immunogen Image HLA-DRB4 Polyclonal Antibody

产品信息

PA5-87621

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000-1:4,000

酶联免疫吸附实验 (ELISA)

1 µg/mL

产品规格

种属反应

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant fusion protein containing a sequence corresponding to amino acids 30-227 of human HLA-DRB4 (NP_0688184)

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1.49 mg/mL

149 µg

纯化类型

Affinity Chromatography

保存液

PBS, pH 7.3, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20°C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

AB_2804296

产品详细信息

Immunogen sequence: GDTQPRFLEQ AKCECHFLNG TERVWNLIRY IYNQEEYARY NSDLGEYQAV TELGRPDAEY WNSQKDLLER RRAEVDTYCR YNYGVVESFT VQRRVQPKVT VYPSKTQPLQ HHNLLVCSVN GFYPGSIEVR WFRNGQEEKA GVVSTGLIQN GDWTFQTLVM LETVPRSGEV YTCQVEHPSM MSPLTVQWSA RSESAQSK; Positive Samples: Raji, Mouse brain; Cellular Location: Cell membrane, Endoplasmic reticulum membrane, Endosome membrane, Golgi apparatus, Late endosome membrane, Lysosome membrane, Single-pass type I membrane protein, trans-Golgi network membrane

靶标信息

HLA-DRB4 belongs to the HLA class II beta chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DRA) and a beta (DRB) chain, both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa and its gene contains 6 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail. Within the DR molecule the beta chain contains all the polymorphisms specifying the peptide binding specificities. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. DRB1 is expressed at a level five times higher than its paralogues DRB3, DRB4 and DRB5. The presence of DRB4 is linked with allelic variants of DRB1, otherwise it is omitted. There are 4 related pseudogenes: DRB2, DRB6, DRB7, DRB8 and DRB9.

HLA and MHC antibodies play a significant role in Immunopeptidomics, facilitating the identification and characterization of neoantigens through high-performance liquid chromatography coupled to tandem Mass Spectrometry.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: DRw53 beta-chain glycoprotein; HLA class II histocompatibility antigen, DR beta 4 chain; HLA-DR11-14N allele; MHC class II antigen; MHC class II antigen DRB4; MHC class II HLA-DR4/w53 protein; MHC DR7-beta-1 chain; MHC HLA-DR-beta chain precursor; MHC HLA-DR-beta-1 chain; unnamed protein product

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基因别名: DR4; DRB4; HLA-DR4B; HLA-DRB; HLA-DRB4; HLA-DRB4*

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UniProt ID: (Human) P13762

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Entrez Gene ID: (Human) 3126

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功能

MHC class II protein complex binding peptide antigen binding

参与通路

adaptive immune response immune system process peptide antigen assembly with MHC class II protein complex antigen processing and presentation of peptide or polysaccharide antigen via MHC class II immune response antigen processing and presentation antigen processing and presentation of exogenous peptide antigen via MHC class II positive regulation of immune response positive regulation of T cell activation