Invitrogen

HSC70 Monoclonal Antibody (13D3)

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29 篇参考文献
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HSC70 Monoclonal Antibody (13D3)

产品信息

MA3-014

应用
建议稀释比
已发表文章

免疫印迹 (WB)

1:1,000-1:4,000
查看15篇已发表文章 15篇已发表文章

免疫组化 (IHC)

-
查看5篇已发表文章 5篇已发表文章

免疫组化（石蜡） (IHC (P))

1:200
-

免疫组化（冰冻） (IHC (F))

1:20-1:200
-

免疫细胞化学 (ICC/IF)

1:250
查看4篇已发表文章 4篇已发表文章

流式细胞分析 (Flow)

-
查看2篇已发表文章 2篇已发表文章

免疫沉淀 (IP)

2 µL
-

中和实验 (Neu)

-
查看1篇已发表文章 1篇已发表文章

竞争反应 (CA)

-
查看1篇已发表文章 1篇已发表文章

其他PubMed (Misc)

-
查看1篇已发表文章 1篇已发表文章
产品规格

种属反应

Cat, Human, Mouse, Non-human primate, Rat

已发表种属

Bovine, C. elegans, Cat, Fish, Human, Mouse, Non-human primate, Rat

宿主/亚型

Mouse / IgM

分类

Monoclonal

类型

Antibody

克隆号

13D3

抗原

Mouse spermatogenic cell protein.
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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Affinity chromatography - MBP

保存液

PBS with 1mg/mL BSA

内含物

0.05% sodium azide

保存条件

-20°C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_325462

产品详细信息

MA3-014 detects heat shock cognate protein 70 kDa (HSC70) from human, feline, primate, rat, and mouse tissues. This antibody displays slight cross-reactivity to HSP70.

MA3-014 has been successfully used in Western blot, immunohistochemistry, immunofluorescence, and immunoprecipitation procedures By Western blot, this antibody detects a 70 kDa protein representing HSC70. By 2D gel electrophoresis, MA3-014 was found to bind strongly to HSC70 and faintly to HSP70 both before and after heat shock. Immunohistochemical staining of HSC70 in mouse spermatids/spermatozoa with MA3-014 results in staining restricted to the post acrosomal region in condensing spermatids and to the midpiece in spermatozoa.

The MA3-014 antigen is mouse spermatogenic cell protein.

靶标信息

The HSP70 family is a set of highly conserved proteins that are induced by a variety of biological stresses, including heat stress, in every organism in which the proteins have been examined. The human HSP70 family members include: HSP70, a protein which is strongly inducible in all organisms but which is also constitutively expressed in primate cells; HSP72, a 72 kDa protein that is induced exclusively under stress conditions; HSC70, or cognate protein, is a 72 kDa, constitutively expressed, protein which is involved in the uncoating of clathrin coated vesicles; GRP78, or BiP, is a glucose regulated 78 kDa protein localized in the endoplasmic reticulum; and p75, or HSP75, a 75 kDa protein that is found within the mitochondria. HSC70 (also known as HSC71, HSC73, HSP73, p72, prp73) is expressed constitutively and is slightly heat-inducible. HSC70 binds to the exposed loop of clathrin light chains to promote uncoating and can also bind the cytoskeleton which may facilitate cytoskeletal rearrangements. HSC70 has been shown to stimulate lysosomal degradation of intracellular proteins and to retard both aggregation and folding of mitochondrial precursor proteins in vitro.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: AAG2; constitutive heat shock protein 70; epididymis luminal protein 33; epididymis secretory sperm binding protein Li 72p; FAM10A1; Heat shock 70 kDa protein 8; heat shock 70kd protein 10; heat shock 70kD protein 8; heat shock 70kDa protein 8; Heat shock cognate 71 kDa protein; heat shock cognate hsc73; heat shock cognate protein 54; Heat shock cognate protein 70; heat shock protein 8; heat shock protein A8; heat shock protein cognate 70; Heat shock protein family A member 8; HIP; LAP; LAP-1; Lipopolysaccharide-associated protein 1; LPS-associated protein 1; MGC131511; MGC29929; N-myristoyltransferase inhibitor protein 71; Protein FAM10A1; SNC6

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基因别名: 2410008N15Rik; HEL-33; HEL-S-72p; HSC54; HSC70; HSC71; Hsc73; HSP71; HSP73; HSPA10; HSPA8; LAP-1; LAP1; NIP71

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UniProt ID: (Human) P11142, (Rat) P63018, (Mouse) P63017

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Entrez Gene ID: (Human) 3312, (Rat) 24468, (Mouse) 15481

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功能
G-protein coupled receptor binding phosphatidylserine binding protein binding ATP binding ATPase activity enzyme binding MHC class II protein complex binding heat shock protein binding ubiquitin protein ligase binding ATPase activity, coupled poly(A) RNA binding unfolded protein binding C3HC4-type RING finger domain binding cadherin binding involved in cell-cell adhesion receptor binding ADP binding nucleotide binding
参与通路
transcription, DNA-templated mRNA processing protein folding RNA splicing protein refolding negative regulation of transcription, DNA-templated positive regulation of mRNA splicing, via spliceosome chaperone mediated protein folding requiring cofactor regulation of cell cycle chaperone-mediated protein folding clathrin coat disassembly negative regulation of fibril organization mRNA splicing, via spliceosome protein targeting to lysosome response to unfolded protein neurotransmitter secretion cellular response to starvation viral process regulation of protein stability regulation of protein complex assembly regulation of mRNA stability ATP metabolic process regulation of protein complex stability chaperone-mediated autophagy late endosomal microautophagy protein targeting to lysosome involved in chaperone-mediated autophagy chaperone-mediated protein transport involved in chaperone-mediated autophagy cell-cell adhesion regulation of cellular response to heat regulation of protein import chaperone-mediated autophagy translocation complex disassembly regulation of transcription, DNA-templated
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