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Invitrogen
HSC70 Monoclonal Antibody (1B5)
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HSC70 Antibody (MA1-26078) in ICC/IF
Immunofluorescence analysis of HSC70 was performed using 70% confluent log phase PC-3 cells treated with Heat Shock (43 degree celsius for 1 hr). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with HSC70 Monoclonal Antibody (1B5) (Product # MA1-26078) at 1 µg/mL concentration in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Rat IgG (H+L) Cross-Adsorbed Second... View More
产品信息
MA1-26078
应用
建议稀释比
已发表文章
产品规格
种属反应
Bovine,
Dog,
Chicken,
Guinea pig,
Hamster,
Human,
Mouse,
Non-human primate,
Sheep,
Pig,
Rabbit,
Rat
已发表种属
C. elegans,
Mouse
宿主/亚型
Rat
/ IgG2a
分类
Monoclonal
类型
Antibody
克隆号
1B5
抗原
Full length native Hsc70 protein (Hamster) purified from sodium arsenite treated heat-resistant variants of Chinese hamster cells.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein G
保存液
PBS, pH 7.2, with 0.1mM PMSF, 50% glycerol
内含物
no preservative
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Recommended positive controls: Bovine Hsc70 Protein and HeLa Heat Shocked Cell Lysate.
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
靶标信息
The HSP70 family is a set of highly conserved proteins that are induced by a variety of biological stresses, including heat stress, in every organism in which the proteins have been examined. The human HSP70 family members include: HSP70, a protein which is strongly inducible in all organisms but which is also constitutively expressed in primate cells; HSP72, a 72 kDa protein that is induced exclusively under stress conditions; HSC70, or cognate protein, is a 72 kDa, constitutively expressed, protein which is involved in the uncoating of clathrin coated vesicles; GRP78, or BiP, is a glucose regulated 78 kDa protein localized in the endoplasmic reticulum; and p75, or HSP75, a 75 kDa protein that is found within the mitochondria. HSC70 (also known as HSC71, HSC73, HSP73, p72, prp73) is expressed constitutively and is slightly heat-inducible. HSC70 binds to the exposed loop of clathrin light chains to promote uncoating and can also bind the cytoskeleton which may facilitate cytoskeletal rearrangements. HSC70 has been shown to stimulate lysosomal degradation of intracellular proteins and to retard both aggregation and folding of mitochondrial precursor proteins in vitro.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: AAG2; constitutive heat shock protein 70; epididymis luminal protein 33; epididymis secretory sperm binding protein Li 72p; FAM10A1; Heat shock 70 kDa protein 8; heat shock 70kd protein 10; heat shock 70kd protein 10 (HSC71); heat shock 70kD protein 8; heat shock 70kDa protein 8; heat shock cognate 70; heat shock cognate 71 kD protein; Heat shock cognate 71 kDa protein; heat shock cognate hsc73; heat shock cognate protein 54; Heat shock cognate protein 70; heat shock protein 8; heat shock protein A8; heat shock protein cognate 70; Heat shock protein family A member 8; HIP; Hsc70 ATPase; LAP; LAP-1; Lipopolysaccharide-associated protein 1; LPS-associated protein 1; MGC131511; MGC29929; N-myristoyltransferase inhibitor protein 71; Protein FAM10A1; SNC6; unnamed protein product
基因别名: 2410008N15Rik; HEL-33; HEL-S-72p; HSC54; HSC70; HSC71; Hsc73; HSP70; HSP71; HSP73; HSPA10; HSPA8; I79_022974; LAP-1; LAP1; NIP71
protein binding
G-protein coupled receptor binding
phosphatidylserine binding
ATP binding
ATPase activity
enzyme binding
MHC class II protein complex binding
heat shock protein binding
ubiquitin protein ligase binding
ATPase activity, coupled
poly(A) RNA binding
unfolded protein binding
C3HC4-type RING finger domain binding
cadherin binding involved in cell-cell adhesion
protein binding involved in protein folding
misfolded protein binding
clathrin-uncoating ATPase activity
receptor binding
ADP binding
nucleotide binding
transcription, DNA-templated
mRNA processing
protein folding
RNA splicing
protein refolding
negative regulation of transcription, DNA-templated
positive regulation of mRNA splicing, via spliceosome
chaperone mediated protein folding requiring cofactor
regulation of cell cycle
chaperone-mediated protein folding
clathrin coat disassembly
negative regulation of fibril organization
synaptic vesicle uncoating
mRNA splicing, via spliceosome
protein targeting to lysosome
response to unfolded protein
neurotransmitter secretion
cellular response to starvation
viral process
regulation of protein stability
regulation of protein complex assembly
regulation of mRNA stability
ATP metabolic process
regulation of protein complex stability
chaperone-mediated autophagy
late endosomal microautophagy
protein targeting to lysosome involved in chaperone-mediated autophagy
chaperone-mediated protein transport involved in chaperone-mediated autophagy
cell-cell adhesion
regulation of cellular response to heat
regulation of protein import
chaperone-mediated autophagy translocation complex disassembly
regulation of transcription, DNA-templated
gastrulation
axo-dendritic transport
embryo development ending in birth or egg hatching
vesicle-mediated transport
cellular response to unfolded protein
embryonic organ development
slow axonal transport
