Invitrogen

HSP90 alpha Polyclonal Antibody

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32个已发表图片
53 篇参考文献
查看其他22个HSP90 alpha抗体

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说明书
实验方案
问题与答案
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HSP90 alpha Polyclonal Antibody

产品信息

PA3-013

应用
建议稀释比
已发表文章

免疫印迹 (WB)

1:500-1:2,000
查看35篇已发表文章 35篇已发表文章

免疫组化 (IHC)

-
查看5篇已发表文章 5篇已发表文章

免疫组化（石蜡） (IHC (P))

5 µg/mL
-

免疫细胞化学 (ICC/IF)

1:50-1:200
查看6篇已发表文章 6篇已发表文章

免疫沉淀 (IP)

2 µg
查看4篇已发表文章 4篇已发表文章

染色质免疫沉淀 实验 (ChIP)

-
查看1篇已发表文章 1篇已发表文章

亲和纯化 (AP)

-
查看2篇已发表文章 2篇已发表文章
产品规格

种属反应

Human, Mouse, Non-human primate, Sheep, Rat

已发表种属

C. elegans, Human, Mouse, Non-human primate, Rabbit

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptide corresponding to residues P(2) E E T Q T Q D Q P M(12) of mouse HSP86.
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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Affinity chromatography

保存液

PBS with 1mg/mL BSA

内含物

0.05% sodium azide

保存条件

-20°C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2120934

产品详细信息

PA3-013 detects heat shock protein 90 (HSP90) from human, monkey, mouse, sheep (adrenal) and rat tissues and cells. This antibody does not detect HSP84.

PA3-013 has been successfully used in Western blot, immunofluorescence, immunohistochemistry (paraffin) and immunoprecipitation procedures. By Western blot, this antibody detects an ~86 kDa protein representing HSP86 from HeLa cell lysate. Immunofluorescence staining of HSP86 in HeLa cells with PA3-013 yields a pattern consistent with cytoplasmic staining. Immunoprecipitation experiments with this antibody suggest that HSP86 exists primarily as homodimers in HeLa cells. Furthermore, the antibody is capable of precipitating HSP86 that is complexed with other proteins such as the aryl hydrocarbon (Ah) receptor.

The PA3-013 immunogen is a synthetic peptide corresponding to residues P(2) E E T Q T Q D Q P M(12) of mouse HSP86. The N-terminal regions of HSP84 and HSP86 show the largest difference in amino acid sequence.

靶标信息

Heat shock proteins (HSP) are proteins that are expressed in response to various biological stress, including heat. HSP90 is a 90 kDa protein that is induced under stress conditions, but is also one of the most abundant cellular proteins found under non-stress conditions. HSP90 has been found to be associated with a number of other intracellular proteins, including steroid receptors, actin, tubulin, and some kinases. It has been suggested that HSP90 is necessary to maintain structural integrity of at least some steroid receptors to allow for ligand binding during receptor activation.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: epididymis luminal secretory protein 52; epididymis secretory sperm binding protein Li 65p; Heat shock 86 kDa; heat shock 90kD protein 1, alpha; heat shock 90kD protein 1, alpha-like 4; heat shock 90kD protein, alpha-like 4; heat shock 90kDa protein 1, alpha; Heat shock protein; heat shock protein 1, alpha; heat shock protein 86; heat shock protein 90kDa alpha (cytosolic), class A member 1; heat shock protein 90kDa alpha family class A member 1; heat shock protein alpha; Heat shock protein family C member 1; Heat shock protein HSP 90-alpha; heat shock protein, 1; heat shock protein, 86 kDa 1; heat shock protein, 89 kDa; Heat shock protein90; HSP; HSP 86; HSP 90 alpha; HSP86; LAP-2; Lipopolysaccharide-associated protein 2; LPS-associated protein 2; Renal carcinoma antigen NY-REN-38; TSTA; Tumor-specific transplantation 86 kDa antigen

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基因别名: 86kDa; 89kDa; AL024080; AL024147; EL52; HEL-S-65p; hsp4; HSP86; Hsp86-1; Hsp89; HSP89A; Hsp90; HSP90A; HSP90AA1; HSP90N; HSPC1; HSPCA; HSPCAL1; HSPCAL4; HSPN; LAP-2; LAP2

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UniProt ID: (Human) P07900, (Mouse) P07901, (Rat) P82995

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Entrez Gene ID: (Human) 3320, (Sheep) 100127209, (Mouse) 15519, (Rat) 299331

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功能
nucleotide binding UTP binding CTP binding mRNA binding protein tyrosine kinase activity protein binding ATP binding GTP binding ATPase activity sulfonylurea receptor binding protein kinase binding protein phosphatase binding MHC class II protein complex binding nitric-oxide synthase regulator activity TPR domain binding dATP binding identical protein binding protein homodimerization activity histone deacetylase binding ion channel binding poly(A) RNA binding GTPase binding Rho GDP-dissociation inhibitor binding unfolded protein binding Hsp90 family chaperone
参与通路
G2/M transition of mitotic cell cycle neuron migration skeletal muscle contraction mitochondrial transport receptor-mediated endocytosis response to unfolded protein signal transduction response to heat response to cold response to salt stress positive regulation of lamellipodium assembly cardiac muscle cell apoptotic process peptidyl-tyrosine phosphorylation regulation of protein ubiquitination positive regulation of protein import into nucleus, translocation Fc-gamma receptor signaling pathway involved in phagocytosis ERBB2 signaling pathway protein refolding regulation of protein complex assembly protein unfolding response to estrogen protein import into mitochondrial outer membrane positive regulation of nitric oxide biosynthetic process positive regulation of cell size response to antibiotic vascular endothelial growth factor receptor signaling pathway protein stabilization regulation of nitric-oxide synthase activity chaperone-mediated protein complex assembly positive regulation of cardiac muscle contraction chaperone-mediated autophagy regulation of cellular response to heat protein folding nitric oxide biosynthetic process response to stress positive regulation of cytotoxic T cell differentiation regulation of catalytic activity
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