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Huntingtin Antibody (MA5-16703) in ICC/IF
Knockdown of HTT was achieved by transfecting SH-SY5Y cells with HTT specific siRNA (Silencer® select Product # s6492, s6491). Immunofluorescence analysis was performed on U-87 MG cells (untransfected, panel a,d), transfected with non-specific scrambled siRNA (panels b,e) and transfected with HTT specific siRNA (panel c,f). Cells were fixed, permeabilized, and labelled with Huntingtin Monoclonal Antibody (H... View More
产品信息
MA5-16703
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rabbit
宿主/亚型
Mouse
/ IgG1
分类
Monoclonal
类型
Antibody
克隆号
HDB4E10
抗原
Recombinant protein corresponding to amino acids 1844 - 2131 of huntingtin.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein A
保存液
PBS
内含物
0.09% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Increased cytoplasmic staining, relative to nuclear, has been reported using formaldehyde as a fixative compared with acetone/methanol. A suggested positive control for immunohistochemical applications is brain.
靶标信息
Huntingtin is a disease gene linked to Huntington's disease (HD), a neurodegenerative disorder characterized by loss of striatal neurons. This is thought to be caused by an expanded, unstable trinucleotide repeat in the huntingtin gene, which translates as a polyglutamine repeat in the protein product. HD is a mid-life onset autosomal dominant neurodegenerative disease that is characterized by psychiatric disorders, dementia, and involuntary movements (chorea), leading to death in 10-20 years.The huntingtin locus is large, spanning 180 kb and consisting of 67 exons. The huntingtin gene is widely expressed and is required for normal development. It is expressed as 2 alternatively polyadenylated forms displaying different relative abundance in various fetal and adult tissues. The larger transcript is approximately 13.7 kb and is expressed predominantly in adult and fetal brain whereas the smaller transcript of approximately 10.3 kb is more widely expressed. The genetic defect leading to Huntington's disease may not necessarily eliminate transcription, but may confer a new property on the mRNA or alter the function of the protein.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: HD protein; HD protein homolog; Huntingtin; Huntington disease gene homolog; Huntington disease protein; huntington disease protein homolog; solute carrier family 6 (neurotransmitter transporter, serotonin), member 4
基因别名: AI256365; C430023I11Rik; HD; Hdh; HTT; IT15
UniProt ID: (Human) P42858
Entrez Gene ID: (Human) 3064, (Rabbit) 100351801, (Mouse) 15194
establishment of mitotic spindle orientation
retrograde vesicle-mediated transport, Golgi to ER
apoptotic process
Golgi organization
central nervous system development
positive regulation of inositol 1,4,5-trisphosphate-sensitive calcium-release channel activity
regulation of protein phosphatase type 2A activity
vocal learning
positive regulation of cilium assembly
vesicle transport along microtubule
animal organ development
mRNA transport
negative regulation of cysteine-type endopeptidase activity
negative regulation of extrinsic apoptotic signaling pathway
urea cycle
citrulline metabolic process
protein import into nucleus
mitochondrial transport
ER to Golgi vesicle-mediated transport
mitochondrion organization
endoplasmic reticulum organization
dopamine receptor signaling pathway
spermatogenesis
gastrulation
brain development
cell aging
learning or memory
learning
grooming behavior
locomotory behavior
axo-dendritic transport
associative learning
determination of adult lifespan
visual learning
anatomical structure morphogenesis
embryo development
anterior/posterior pattern specification
endosomal transport
lactate biosynthetic process from pyruvate
quinolinate biosynthetic process
striatum development
olfactory lobe development
neural plate formation
neurogenesis
peptide hormone secretion
insulin secretion
social behavior
hormone metabolic process
negative regulation of apoptotic process
negative regulation of neuron apoptotic process
regulation of mitochondrial membrane permeability
regulation of synaptic plasticity
paraxial mesoderm formation
neuron development
neuron apoptotic process
response to calcium ion
regulation of mitochondrial membrane potential
L-glutamate import
iron ion homeostasis
negative regulation of neuron death
