Invitrogen

IL-6 Monoclonal Antibody (MP5-32C11), Biotin, eBioscience™

Name: IL-6 Monoclonal Antibody (MP5-32C11), Biotin, eBioscience\u2122
Brand: Invitrogen
SKU: 13-7062-81

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31 篇参考文献

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说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

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IL-6 Antibody (13-7062-81) in ELISA

Journal of biomedical science 2021 - Fig. 6 5-MTP suppresses HFD-induced IL-6 and HSP60 in the atherosclerotic lesions of ApoE -/- mice. Chow diet-fed or HFD-fed ApoE -/- mice were treated with saline or L-5-MTP twice weekly for 20 wk. ( A , B ) IL-6 and ( C , D ) OPG in paraffin-embedded aortic tissues were analyzed by immunohistochemistry. Negative control represents staining with a 2nd antibody. Representative microphotographs of the immuno... View More

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IL-6 Antibody — Figure 4 Peli1 mediates LPS-induce inflammatory responses in microglia. ( a ) QPCR analysis of relative mRNA expression for the indicated inflammatory genes in the primary cultured microglia isolated from WT and Peli1 -KO mice, left untreated (NT) or stimulated with LPS (100 ng/ml). ( b ) ELISA of IL-1beta, IL-6, TNF and nitrites in the supernatant of WT and Peli1 -KO microglia cultures that left untreated (NT) or stimulated with LPS (100 ng/ml) at the indicated time points. ( c , d ) QPCR analysis of relative mRNA expression for the indicated inflammatory genes in the primary cultured microglia ( c ) or astrocytes ( d ) isolated from WT and Peli1 -KO mice, left untreated (NT) or stimulated with IL-1beta (20 ng/ml). The gene expression were normalized to a reference gene Actb (encoding beta-actin) and showed as the relative value to Actb in bar graphs. Data with error bars represent mean +- SD. Each panel is representative of at least three independent experiment. *P < 0.05, **P < 0.01 as determined by two-way ANOVA with a Bonferroni post test. {TM}

产品信息

13-7062-81

应用

建议稀释比

已发表文章

酶联免疫吸附实验 (ELISA)

0.5-2 µg/mL

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中和实验 (Neu)

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产品规格

种属反应

Mouse

已发表种属

C. elegans, Human, Mouse

宿主/亚型

Rat / IgG2a, kappa

分类

Monoclonal

类型

Antibody

克隆号

MP5-32C11

偶联物

Biotin Biotin Biotin

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4°C, do not freeze

运输条件

Ambient (domestic); Wet ice (international)

AB_466910

产品详细信息

Description: The MP5-32C11 antibody reacts with mouse interleukin-6 (IL-6), a 21-28 kDa cytokine secreted by a variety of cell types.

Applications Reported: MP5-32C11 has been reported for use in detection of mouse IL-6 by ELISA and ELISPOT.

Applications Tested: The MP5-32C11 antibody has been tested as the detection antibody in a sandwich ELISA for analysis of mouse Interleukin-6 (IL-6) in combination with the purified MP5-20F3 (14-7061) antibody for capture and recombinant mouse IL-6 (14-8061) as the standard. A suitable range of concentrations of this antibody for ELISA detection is 0.5-2 µg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 1000 pg/mL - 8 pg/mL should be included in each ELISA plate.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

IL-6 is a gene that encodes a cytokine that plays a role in inflammation and the maturation of B cells. The protein produced by this gene is an endogenous pyrogen that can induce fever in people with autoimmune diseases or infections. The gene is primarily expressed at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. Elevated levels of the encoded protein have been found in virus infections, including COVID-19 (disease caused by SARS-CoV-2). Diseases associated with IL6 include Kaposi Sarcoma and Rheumatoid Arthritis, Systemic Juvenile.

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