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Invitrogen
LAMTOR1 Polyclonal Antibody
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FIGURE: 1 / 7
LAMTOR1 Antibody (PA5-23086) in ICC/IF
Immunofluorescence analysis of Ragulator complex protein LAMTOR1 was performed using 70% confluent log phase Hep G2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with LAMTOR1 Polyclonal Antibody (Product # PA5-23086) at 1:100 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor P... View More
Product Details
PA5-23086
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A genomic peptide made to a C-terminal region of the human c11orf59/LAMTOR1 protein (within residues 50-250).
Conjugate
Form
Liquid
Concentration
0.2 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 50% glycerol, 0.1% BSA
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
The target sequence has 98% sequence homology with bovine.
Target Information
The Ragulator complex is comprised of three proteins, C11orf59, ROBLD3, and MAPKSP1, which targets mTORC1 to lysosomal surfaces. This normal endosomal trafficking is taken advantage of by certain viruses, including coronavirus, which has increased replication when C11orf59 is interfered with. C11orf59 is positively regulated by cellular cholesterol depletion and negatively regulated by cellular cholesterol loading. It is involved in LDL uptake and cholesterol homeostasis. C11orf59 and PPAR-alpha work together to control lipid and carbohydrate metabolism in diabetes. Nearly all effects of C11orf59 are associated with mTORC1 kinase growth promotion, and disregulation of the normal mTOR pathway.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Late endosomal/lysosomal adaptor and MAPK and MTOR activator 1; Lipid raft adaptor protein p18; p27kip1 releasing factor from RhoA; p27Kip1-releasing factor from RhoA; p27RF Rho; p27RF-Rho; Protein associated with DRMs and endosomes; Ragulator complex protein LAMTOR1; ragulator complex protein PDRO; RhoA activator C11orf59; RhoA activator C11orf59 homolog
Gene Aliases: 2400001E08Rik; C11orf59; LAMTOR1; p18; p27RF-Rho; PDRO; PP7157; Ragulator1
UniProt ID: (Human) Q6IAA8, (Rat) Q6P791, (Mouse) Q9CQ22
Entrez Gene ID: (Human) 55004, (Rat) 308869, (Mouse) 66508
regulation of receptor recycling
endosome organization
lysosome organization
regulation of cholesterol esterification
regulation of cholesterol efflux
cell growth
positive regulation of TOR signaling
lysosome localization
endosome localization
cellular protein localization
cholesterol homeostasis
positive regulation of MAPK cascade
positive regulation of GTPase activity
regulation of cholesterol import
cellular response to amino acid stimulus
cell cycle arrest
macroautophagy
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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