Search
Invitrogen
LEFTY Polyclonal Antibody
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
{{$ctrl.translations['antibody.pdp.skuNotes.additionalInformation']}}:
FIGURE: 1 / 1
LEFTY Antibody (PA5-90431) in WB
Western blot analysis of LEFTY in extracts of various cell lines. Samples were incubated with LEFTY Polyclonal antibody (Product # PA5-90431) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-90431
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 77-366 of human LEFTY1 (NP_0662771)
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1.27 mg/mL
Amount
127 µg
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: RFSQSFREVA GRFLALEAST HLLVFGMEQR LPPNSELVQA VLRLFQEPVP KAALHRHGRL SPRSARARVT VEWLRVRDDG SNRTSLIDSR LVSVHESGWK AFDVTEAVNF WQQLSRPRQP LLLQVSVQRE HLGPLASGAH KLVRFASQGA PAGLGEPQLE LHTLDLGDYG AQGDCDPEAP MTEGTRCCRQ EMYIDLQGMK WAENWVLEPP GFLAYECVGT CRQPPEALAF KWPFLGPRQC IASETDSLPM IVSIKEGGRT RPQVVSLPNM RVQKCSCASD GALVPRRLQP; Positive Samples: HT-29, SW480, BxPC-3, Mouse pancreas, Mouse spleen, Rat pancreas
Target Information
This gene encodes a member of the TGF-beta family of proteins. A similar secreted protein in mouse plays a role in left-right asymmetry determination of organ systems during development. Alternative processing of this protein can yield three different products. This gene is closely linked to both a related family member and a related pseudogene.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: left-right determination factor B; left-right determination, factor B; protein lefty-1; protein lefty-B; stimulated by retinoic acid gene 3 protein; TGF-beta-4; transforming growth factor beta-4; unnamed protein product
Gene Aliases: Ebaf; LEFTB; lefty; lefty-1; LEFTYB; Stra3; Tgfb4
UniProt ID: (Human) O75610, (Mouse) Q64280
Entrez Gene ID: (Human) 10637, (Mouse) 13590
negative regulation of transcription from RNA polymerase II promoter
heart morphogenesis
determination of left/right symmetry
negative regulation of cell proliferation
anterior/posterior axis specification
BMP signaling pathway
response to retinoic acid
cell migration involved in gastrulation
negative regulation of nodal signaling pathway
negative regulation of nodal receptor complex assembly
transforming growth factor beta receptor signaling pathway
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support