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Agrisera
LFY/Leafy Polyclonal Antibody
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LFY/Leafy Antibody (AS16-3990) in WB
20 µg of total protein: Markers (1), 35 S: : LFY lines tsp 1 (2), 35 S: : LFY lines E 1 (3), 35 S: : LFY lines tsp 2 (4), 35 S: : LFY lines E 2 (5), Empty well (6), 35 S: : LFY lines tsp 3 (7), 35 S: : LFY lines E 3 (8), LFY recombinant protein (9) extracted freshly from leaves with IP buffer (Co-IP kit from Invitrogen) and denatured with SDS reducing dye at 90°C for 5 min were separated on 12% SDS-PAGE and blotted for 10 min to PVDF using dry transfer system (i Blot, Invitrogen). Blot was blocked with blocking buffer for 10 min at RT with... View More
产品信息
AS16-3990
产品规格
种属反应
Plant
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
KLH-conjugated synthetic peptide derived from LFY sequence of Arabidopsis thaliana.
偶联物
形式
Lyophilized
规格
50 µg
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7.4
内含物
no preservative
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
For reconstitution add 50 µL of sterile water.
Specific Species Reactivity: Arabidopsis thaliana
靶标信息
Leafy encodes a nuclear, land plant-specific transcription factor that functions as a master regulator of floral meristem identity, promoting the transition from inflorescence to flower formation and coordinating multiple aspects of floral morphogenesis, including organ number, identity, and determinacy, in large part by activating key floral commitment and patterning programs such as APETALA1 and, in specific contexts, contributing to regulation of class B genes via cofactor interactions (e.g., with UFO at relevant promoters). The gene produces a protein with an N-terminal conserved self-association module that mediates homo-oligomerization (including a Sterile Alpha Motif-like domain implicated in multimerization) and a highly conserved C-terminal DNA-binding domain with a helix-turn-helix-like fold that binds semi-palindromic target elements as a dimer; together these domains shape genome-wide binding and transcriptional output. Beyond sequence-specific transcriptional control, LEAFY can exhibit pioneer transcription factor behavior by engaging nucleosome-occupied sites and facilitating local chromatin unlocking, supporting cell fate reprogramming toward floral identity.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: AT5G61850; LEAFY; LEAFY 3; LFY; LFY3; MAC9.13; MAC9_13
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