Invitrogen

LRP5 Polyclonal Antibody

Name: LRP5 Polyclonal Antibody
Brand: Invitrogen
SKU: 36-5400

高级验证

6个已发表图片

10 篇参考文献

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说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

LRP5 Antibody in Immunocytochemistry (ICC/IF)

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LRP5 Antibody (36-5400) in ICC/IF

Immunofluorescent staining of LRP5-transfected 293T cells using Rb anti-LRP5 (Product # 36-5400). Image courtesy of Heekyung Chung, PhD, and Anthony Brown, PhD, Weill Medical College of Cornell University, NY.

LRP5 Antibody in Immunohistochemistry (IHC)

Product Image Gallery

LRP5 Antibody — FIG. 5. Dkk1 treatment and LRP5/LRP6 siRNA stimulated neurite outgrowth. (A) Wnt-1 and Wnt-3a CM promoted beta-catenin stabilization in TC-32 cells. Stabilization of free beta-catenin was analyzed by GST-E-cadherin pull-down assay. Immunoblot (IB) analyses of beta-catenin pelleted with GST-E-cadherin and of beta-catenin and HSP70 in whole-cell lysates are indicated. -, absence of; +, presence of. (B) Dkk1 blocked Wnt-3a-induced beta-catenin stabilization in TC-32 cells. Cells were serum starved overnight and pretreated with Dkk1 for 30 min at 37degC in a CO 2 incubator. Wnt-3a CM or control L (Ctl.) CM were then added, and cells were further incubated for 3 h, followed by GST-E-cadherin pull-down assay. (C) Dkk1 induced neurite outgrowth. TC-32 cells plated on coverslips were pretreated with Dkk1 as indicated and subsequently incubated with Wnt-3a or control medium for 3 h. Then cells were fixed and stained with Alexa Fluor 568 phalloidin. Scale bars, 20 mum. (D) Quantitative analysis of the effect of Dkk1, Wnt-3a, and cotreatment with Dkk1 and Wnt-3a on neurite outgrowth. The percentage of cells having neurites was determined for each treatment group. The stimulation of neurite outgrowth for each group was defined as the ratio of this percentage to the percentage observed for the negative control. The results are means +- standard deviations (error bars) from three independent experiments. ***, P < 0.001. (E) Knockdown of LRP5 and LRP6 in TC-32 cells. Cells were transfected {KD}

Immunogen image

Immunogen Image LRP5 Polyclonal Antibody

产品信息

36-5400

应用

建议稀释比

已发表文章

免疫印迹 (WB)

Assay-dependent

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免疫组化 (IHC)

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免疫组织化学（多聚甲醛固定） (IHC (PFA))

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免疫细胞化学 (ICC/IF)

Assay-dependent

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产品规格

种属反应

Human, Mouse

已发表种属

C. elegans, Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptide derived from the C-terminal region of the human LRP5 protein. This intracellular region is identical to the corresponding mouse and rat LRP5 amino acid sequences.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.25 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7.4

内含物

0.1% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_2533267

靶标信息

LDL related proteins, LRP 5 and LRP 6, are members of the LDL receptor superfamily, which were found to be involved in the canonical Wnt signaling pathway. Mammalian LRP 6 was shown to bind to Wnt 1 and enhance Wnt-induced developmental processes in Xenopus embryos. Mice lacking LRP 6 exhibited developmental defects that are similar to those caused by deficiencies in various Wnt proteins. Recent work also shows that Wnt proteins induce the binding of LRP 5 to Axin, leading Axin degradation and beta catenin stabilization.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: HGNC:6697; low density lipoprotein receptor-related protein 5; low density lipoprotein receptor-related protein 7; Low-density lipoprotein receptor-related protein 5; Low-density lipoprotein receptor-related protein 7; LRP-5; LRP-7

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基因别名: BMND1; EVR1; EVR4; HBM; LR3; LRP-5; LRP5; LRP7; mKIAA4142; OPPG; OPS; OPTA1; VBCH2

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UniProt ID: (Human) O75197, (Mouse) Q91VN0

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Entrez Gene ID: (Human) 4041, (Mouse) 16973

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功能

protein binding Wnt-protein binding toxin transporter activity Wnt-activated receptor activity coreceptor activity involved in Wnt signaling pathway coreceptor activity involved in canonical Wnt signaling pathway transmembrane signal receptor

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