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Invitrogen
HLA-G Monoclonal Antibody (87G)
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FIGURE: 1 / 3
HLA-G Antibody (MA1-10356) in Flow
Flow Cytometry analysis of HLA-G using HLA-G Monoclonal Antibody (87G) (Product # MA1-10356). Separation of HLA-G transfected LCL cells (red-filled) from non-transfected LCL cells (black-dashed) in flow cytometry analysis (surface staining) stained using anti-HLA-G (87G) purified antibody (concentration in sample 10 μg/mL, GAM APC).
Product Details
MA1-10356
Applications
Tested Dilution
Publications
Product Specifications
Published species
Human,
Pig
Host/Isotype
Mouse
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
87G
Immunogen
HLA-B27 transgenic mice were imunized with H-2 identical murine cells transfected with and expressing genes encoding HLA-G and human beta2-microglobulin.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Storage conditions
4°C, do not freeze
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody recognizes both membrane-bound and soluble forms of HLA-G (HLA-G1 and HLA-G5). HLA-G belongs to the MHC Class I molecules (MHC Class Ib; nonclassical) and it is expressed on the surface of trophoblast cells.
It will not cross-react with mouse or rat.
Endotoxin level is less than 0.01 EU/ug of the protein, as determined by the LAL test.
Immunohistochemistry (Frozen): Incubation: 20 min at 25°C; Functional Application: Blocking.
Target Information
HLA-G belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA-G is expressed on fetal derived placental cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domain, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exon 6 encodes the cytoplasmic tail.
HLA and MHC antibodies play a significant role in Immunopeptidomics, facilitating the identification and characterization of neoantigens through high-performance liquid chromatography coupled to tandem Mass Spectrometry.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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