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          • Primary Antibodies ›
          • HIF1A Antibodies

          Invitrogen

          HIF1A Monoclonal Antibody (H1alpha67)

          10 Published Figures
          66 References
          View all (67) HIF1A antibodies

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          Cite HIF1A Monoclonal Antibody (H1alpha67)

          Additional Information:
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          • Antibody Testing Data (3)
          • Published Figures (10)
          HIF1A Antibody in Immunocytochemistry (ICC/IF)
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          HIF1A Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 13

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          HIF1A Antibody (MA1-16504) in ICC/IF

          Immunocytochemistry analysis of HIF1A in immersion fixed HeLa human cervical epithelial carcinoma cell line treated with DFOA. Samples were incubated with HIF1A monoclonal antibody (Product # MA1-16504) using a dilution of 1 µg/mL followed by donkey anti-rabbit. Counterstained with DAPI (blue). {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          View Product

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          HIF1A Antibody in Immunocytochemistry (ICC/IF)
          HIF1A Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          HIF1A Antibody in Western Blot (WB)
          HIF1A Antibody in Immunohistochemistry (IHC)
          HIF1A Antibody in Immunohistochemistry, Immunohistochemistry (Paraffin) (IHC, IHC (P))
          HIF1A Antibody in Immunohistochemistry (IHC)
          HIF1A Antibody in Immunohistochemistry (IHC)
          HIF1A Antibody in Immunohistochemistry (PFA fixed) (IHC (PFA))
          HIF1A Antibody in Western Blot (WB)
          HIF1A Antibody in Western Blot (WB)
          HIF1A Antibody in Western Blot (WB)
          HIF1A Antibody in Western Blot (WB)
          HIF1A Antibody in ChIP Assay (ChIP)

          Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

          HIF1A Monoclonal Antibody (H1alpha67)

          Product Details

          MA1-16504

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:500
          View 16 publications 16 publications

          Immunohistochemistry (IHC)

          -
          View 39 publications 39 publications

          Immunohistochemistry (Paraffin) (IHC (P))

          1:20-1:50
          View 5 publications 5 publications

          Immunohistochemistry (PFA fixed) (IHC (PFA))

          -
          View 1 publication 1 publication

          Immunohistochemistry (Frozen) (IHC (F))

          1:20-1:50
          -

          Immunocytochemistry (ICC/IF)

          1:50
          View 1 publication 1 publication

          Flow Cytometry (Flow)

          1:10-1:1,000
          -

          Immunoprecipitation (IP)

          1:10-1:500
          -

          ChIP assay (ChIP)

          1-5 µg
          View 1 publication 1 publication

          Gel Shift (EMSA)

          -
          View 3 publications 3 publications
          Product Specifications

          Species Reactivity

          Bovine, Dog, Cat, Ferret, Human, Mouse, Non-human primate, Sheep, Pig, Rabbit, Rat, Xenopus

          Published species

          Bovine, Horse, Human, Mouse, Pig, Rabbit, Rat, Sheep

          Host/Isotype

          Mouse / IgG2b

          Class

          Monoclonal

          Type

          Antibody

          Clone

          H1alpha67

          Immunogen

          Fusion protein containing amino acids 432-528 of human HIF-1 alpha.
          3D Epitope / Immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated
          • Biotin 
          • DyLight 488
          • DyLight 550
          • DyLight 650
          • Request custom conjugation

          Form

          Liquid

          Concentration

          1.0 mg/mL

          Amount

          100 µg

          Purification

          Protein G

          Storage buffer

          PBS with 1% BSA

          Contains

          0.05% sodium azide

          Storage conditions

          Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_568567

          Product Specific Information

          In Western blot, multiple bands may be seen at 100-120 kDa representing post-translational modification of HIF-1 alpha.

          For WB, testing on nuclear extracts is recommended.

          Recommended positive controls: COS-7 nuclear hypoxic induced cell lysate, HeLa hypoxic (CoCl2) cell lysate, HepG2 hypoxic (CoCl2) cell lysate, HeLa hypoxic cell lysate, HepG2 hypoxic cell lysate, HIF-1 alpha Knockout HeLa cell lysate

          Target Information

          HIF1-alpha (HIF1A) is a subunit of HIF1, which is a transcription factor found in mammalian cells cultured under reduced oxygen tension. HIF-1 is a heterodimer consisting of an alpha and beta subunit, both belonging to the basic-helix-loop-helix Per-aryl hydrocarbon receptor nuclear translocator-Sim (PAS) family of transcription factors. HIF1 functions as a transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions, HIF-1 activates the transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, HILPDA, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. HIF1-alpha regulates hypoxia-mediated apoptosis, cell proliferation and tumor angiogenesis. Hypoxia which induces p53 protein accumulation, directly interacts with HIF1-alpha and reduces hypoxia-induced expression of HIF1-alpha by promoting MDM2-mediated ubiquitination and proteasomal degradation under hypoxic conditions. Recent studies suggest that induction of NOX4 by HIF1-alpha contributes to maintain ROS levels after hypoxia and hypoxia-induced proliferation. In humans, it is located on the q arm of chromosome 14. The C-terminal of HIF1A binds to p300. p300/CBP-HIF complexes participate in the induction of hypoxia-responsive genes, including VEGF. Hypoxia contributes significantly to the pathophysiology of major categories of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease and chronic obstructive pulmonary disease.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: ARNT-interacting protein; ARNT2; Basic-helix-loop-helix-PAS protein MOP1; bHLHe78; Class E basic helix-loop-helix protein 78; HIF; hif 1; hif 1a; HIF-1 alpha; HIF-1-alpha; HIF1 alpha; HIF1-alpha; hypoxia inducible factor 1 alpha subunit; hypoxia inducible factor 1 alpha subunit L homeolog; hypoxia inducible factor 1, alpha subunit; hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor); Hypoxia-inducible factor 1 alpha; hypoxia-inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor); hypoxia-inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor) L homeolog; Hypoxia-inducible factor 1-alpha; Member of PAS protein 1; PAS domain-containing protein 8

          View more View less

          Gene Aliases: AA959795; BHLHE78; hif-1alpha; HIF1A; HIF1alpha; MOP1; PASD8; XELAEV_18039792mg; XELAEV_18041137mg; xhif1a

          View more View less

          UniProt ID: (Mouse) Q61221, (Rat) O35800, (Xenopus) Q9I8A9

          View more View less

          Entrez Gene ID: (Bovine) 281814, (Pig) 396696, (Sheep) 443519, (Dog) 480348, (Rabbit) 100009579, (Mouse) 15251, (Rat) 29560, (Xenopus) 100337573, (Xenopus) 445838, (Xenopus) 380141

          View more View less

          Function(s)
          transcription factor activity, sequence-specific DNA binding histone acetyltransferase binding sequence-specific DNA binding protein heterodimerization activity RNA polymerase II transcription factor activity, sequence-specific DNA binding transcription factor activity, transcription factor binding transcription factor activity, RNA polymerase II transcription factor binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding DNA binding transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding signal transducer activity protein binding transcription factor binding enzyme binding protein kinase binding ubiquitin protein ligase binding protein complex binding nuclear hormone receptor binding histone deacetylase binding protein dimerization activity Hsp90 protein binding double-stranded DNA binding basic helix-loop-helix transcription factor
          Process(es)
          response to hypoxia positive regulation of vascular endothelial growth factor production axonal transport of mitochondrion positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter positive regulation of transcription from RNA polymerase II promoter in response to hypoxia cellular response to hypoxia angiogenesis blood vessel development neural crest cell migration epithelial to mesenchymal transition embryonic placenta development B-1 B cell homeostasis vasculature development heart looping positive regulation of neuroblast proliferation connective tissue replacement involved in inflammatory response wound healing outflow tract morphogenesis cardiac ventricle morphogenesis lactate metabolic process regulation of glycolytic process transcription, DNA-templated regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase II promoter cellular iron ion homeostasis signal transduction lactation positive regulation of cell proliferation visual learning regulation of gene expression positive regulation of autophagy vascular endothelial growth factor production positive regulation of gene expression positive regulation of epithelial cell migration positive regulation of receptor biosynthetic process response to muscle activity neural fold elevation formation cerebral cortex development cell differentiation negative regulation of bone mineralization positive regulation of vascular endothelial growth factor receptor signaling pathway negative regulation of TOR signaling oxygen homeostasis regulation of transforming growth factor beta2 production collagen metabolic process negative regulation of transcription elongation from RNA polymerase II promoter embryonic hemopoiesis positive regulation of insulin secretion involved in cellular response to glucose stimulus regulation of cell proliferation hemoglobin biosynthetic process glucose homeostasis positive regulation of apoptotic process negative regulation of apoptotic process negative regulation of neuron apoptotic process regulation of transcription from RNA polymerase II promoter in response to oxidative stress positive regulation of erythrocyte differentiation negative regulation of vasoconstriction negative regulation of growth muscle cell cellular homeostasis positive regulation of hormone biosynthetic process blood vessel morphogenesis digestive tract morphogenesis positive regulation of smooth muscle cell proliferation regulation of catalytic activity cartilage development elastin metabolic process intestinal epithelial cell maturation response to fungicide epithelial cell differentiation involved in mammary gland alveolus development retina vasculature development in camera-type eye regulation of thymocyte apoptotic process negative regulation of thymocyte apoptotic process dopaminergic neuron differentiation hypoxia-inducible factor-1alpha signaling pathway negative regulation of oxidative stress-induced neuron intrinsic apoptotic signaling pathway positive regulation of mitophagy regulation of aerobic respiration negative regulation of reactive oxygen species metabolic process negative regulation of mesenchymal cell apoptotic process response to mechanical stimulus response to salt stress response to glucose response to X-ray response to auditory stimulus response to purine-containing compound response to activity response to cobalt ion response to estradiol cellular response to insulin stimulus cellular response to drug response to drug response to alkaloid response to estrogen positive regulation of cell size response to glucocorticoid maternal process involved in female pregnancy cellular response to hydrogen peroxide cellular response to lipopolysaccharide cellular response to carbon monoxide cellular response to nitrite cellular response to electrical stimulus cellular response to mechanical stimulus cellular response to cobalt ion cellular response to glucose stimulus cellular response to interleukin-1 cellular response to lipid cellular response to organic cyclic compound cellular response to light stimulus response to anesthetic cellular response to toxic substance regulation of cellular response to hypoxia cellular response to cyanide
          It has to be done as per old AB suggested Products section.

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