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Invitrogen
SIT Monoclonal Antibody (SIT-01), PE
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FIGURE: 1 / 3
SIT Antibody (MA1-19652) in Flow
Flow cytometry (intracellular staining) of SIT on human peripheral whole blood. Samples were stained with SIT PE monoclonal antibody (Product # MA1-19652) using a dilution of 5 μg/mL. Separation of human CD3 positive SIT positive T cells (red-filled) from CD3 negative SIT negative lymphocytes (black-dashed).
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA1-19652
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
SIT-01
Immunogen
Bacterially produced recombinant intracellular fragment of human SIT.
Conjugate
PE
PE
PE
Excitation/Emission Max
565/576 nm
View spectra
Form
Liquid
Concentration
0.1 mg/mL
Amount
100 µg
Purification
Size-exclusion chromatography
Storage buffer
PBS, pH 7.4, with 0.2% BSA
Contains
15mM sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody reacts with murine SIT.
Target Information
SIT (SHP2-interacting transmembrane adaptor protein) is expressed exclusively in lymphoid organs and acts either as a positive or as a negative regulatory element in T cell activation and in T cell development. Binding to Grb2 plays a pivotal role in signal transduction. Hubener et al. (2001) determined that the SIT gene contains 5 exons and spans 1.8 kb of genomic DNA. The SIT promoter demonstrated strong transcriptional activity and potential binding sites for both ubiquitous and lymphoid-specific transcription factors.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: gp30/40; MGC125908; MGC125909; MGC125910; RP11-331F9.5; SHP2-interacting transmembrane adapter protein; Signaling threshold-regulating transmembrane adapter 1; Suppression-inducing transmembrane adapter 1
Gene Aliases: SIT; SIT1
Entrez Gene ID: (Human) 27240
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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