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c-Myc Antibody (MA1-980-A647) in ICC/IF
Immunofluorescent analysis of HeLa cells transfected with a construct containing a MYC Epitope Tag. Cells were fixed with 4% paraformaldehyde, permeabilized with 0.1% Triton X-100 inPBS for 15 minutes at room temperature and blocked with 3% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were stained with AlexaFluor 647 conjugated MYC-tag monoclonal antibody (Product # MA1-980-A647) at a dilution of 1:25 for 1 hour at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye (Product # 62249). Images were ... View More
产品信息
MA1-980-A647
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
已发表种属
Human
宿主/亚型
Mouse
/ IgG1
分类
Monoclonal
类型
Antibody
克隆号
9E10
抗原
Synthetic peptide A(408) E E Q K L I S E E D L L R K R R E Q L K H K L E Q L R N S C A(438) of human c-Myc
偶联物
激发/发射光谱
650/671 nm
查看光谱
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein A
保存液
PBS with 1mg/mL BSA
内含物
0.05% sodium azide
保存条件
4° C, do not freeze
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2609821
靶标信息
The c-Myc protein is a 62 kDa transcription factor that is encoded by the c-Myc gene on human chromosome 8q24. c-Myc is commonly activated in a variety of tumor cells and plays an important role in cellular proliferation, differentiation, apoptosis and cell cycle progression. The phosphorylation of c-Myc has been investigated and previous studies have suggested a functional association between phosphorylation at Thr58/Ser62 by glycogen synthase kinase 3, cyclin dependent kinase, ERK2 and C-Jun N terminal Kinase (JNK) in cell proliferation and cell cycle regulation. Studies have shown that c-Myc is essential for vasculogenesis and angiogenesis in tumor development, which distributes blood throughout the cells. The c-myc oncogene (p62 c-myc) is involved in the control of normal cellular proliferation and differentiation, and the deregulated expression of c-Myc induces apoptosis in different cell types. Antibodies against c-myc epitopes recognize overexpressed proteins containing Myc epitope tag fused to either amino- or carboxy-termini of targeted proteins. c-Myc-, N-Myc- and L-Myc-encoded proteins function in cell proliferation, differentiation and neoplastic disease. Amplification of the c-Myc gene has been found in several types of human tumors including lung, breast and colon carcinomas, while the N-Myc gene has been found amplified in neuroblastomas. Translocation of the c-myc locus on chromosome 8 to the immunoglobulin loci on chromosome 14 (heavy chain); 2 (delta light chain); or 22 (light chain) is described in Burkitts lymphoma and other B-cell lymphoproliferative conditions. An aberrant expression of the c-myc gene occurs in tumors of different origins such as colorectal, gastric, gallbladder, hepatic, mammary, ovarian, endometrial, head and neck, pulmonary, prostatic, thyroidal, oral, ocular, nasopharyngeal, endocrine, as well as hematopoietic neoplasms.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: avian myelocytomatosis viral oncogene homolog; bHLHe39; c Myc; c-myc epitope tag; Class E basic helix-loop-helix protein 39; cmyc; Myc proto-oncogene protein; myc-related translation/localization regulatory factor; Proto-oncogene c-Myc; Transcription factor p64; v-myc myelocytomatosis viral oncogene homolog
基因别名: BHLHE39; c-Myc; MRTL; MYC; MYCC
UniProt ID: (Human) P01106
Entrez Gene ID: (Human) 4609
RNA polymerase II core promoter proximal region sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
DNA binding
transcription factor activity, sequence-specific DNA binding
protein binding
transcription factor binding
protein complex binding
protein dimerization activity
repressing transcription factor binding
E-box binding
basic helix-loop-helix transcription factor
negative regulation of transcription from RNA polymerase II promoter
MAPK cascade
branching involved in ureteric bud morphogenesis
positive regulation of mesenchymal cell proliferation
energy reserve metabolic process
chromatin remodeling
transcription from RNA polymerase II promoter
cellular iron ion homeostasis
cellular response to DNA damage stimulus
cell cycle arrest
Notch signaling pathway
positive regulation of cell proliferation
response to gamma radiation
regulation of gene expression
oxygen transport
regulation of telomere maintenance
negative regulation of stress-activated MAPK cascade
cellular response to UV
cellular response to drug
response to drug
negative regulation of apoptotic process
positive regulation of cysteine-type endopeptidase activity involved in apoptotic process
fibroblast apoptotic process
negative regulation of monocyte differentiation
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
positive regulation of fibroblast proliferation
negative regulation of fibroblast proliferation
positive regulation of epithelial cell proliferation
chromosome organization
negative regulation of cell division
canonical Wnt signaling pathway
response to growth factor
positive regulation of metanephric cap mesenchymal cell proliferation
beta-catenin-TCF complex assembly
positive regulation of DNA biosynthetic process
positive regulation of response to DNA damage stimulus
