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          • Primary Antibodies ›
          • HIF1A Antibodies

          Invitrogen

          HIF1A Monoclonal Antibody (ESEE122), DyLight™ 488

          1 Published Figure
          2 References
          View all (67) HIF1A antibodies

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          Cite HIF1A Monoclonal Antibody (ESEE122), DyLight™ 488

          Additional Information:
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          • Published Figures (1)
          HIF1A Antibody in Immunohistochemistry (Paraffin) (IHC (P))
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          HIF1A Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Group 53 Created with Sketch.

          FIGURE: 1 / 1

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          HIF1A Antibody (MA5-16018) in IHC (P)

          MethodsX 2023 - Fig. 1 IHC staining of U251 tumoroid cytoskeleton with actin (a), autophagosome with LC3 (b, c), and hypoxia with Hif1-alpha following the presented protocol (d-1) compared with the tumoroids underwent conventional intermediate steps of washing and centrifugation (d-2). Tumoroids were counterstained with DAPI. Scale bars are 100 um. Fig. 1 {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
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          View Product

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          HIF1A Antibody in Immunohistochemistry (Paraffin) (IHC (P))

          Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

          HIF1A Monoclonal Antibody (ESEE122), DyLight™ 488

          Product Details

          MA5-16018

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          Assay-Dependent
          -

          Immunohistochemistry (IHC)

          -
          View 1 publication 1 publication

          Immunohistochemistry (Paraffin) (IHC (P))

          1:100-1:5000
          View 1 publication 1 publication

          Immunohistochemistry (Frozen) (IHC (F))

          1:100-1:5000
          -

          Immunocytochemistry (ICC/IF)

          10 µg/mL
          -

          Flow Cytometry (Flow)

          Assay-Dependent
          -

          Immunoprecipitation (IP)

          Assay-dependent
          -
          Product Specifications

          Species Reactivity

          Bovine, Dog, Human, Mouse, Rat

          Published species

          Human

          Host/Isotype

          Mouse / IgG1

          Class

          Monoclonal

          Type

          Antibody

          Clone

          ESEE122

          Immunogen

          Human HIF-1 alpha corresponding to amino acids 329-530.
          3D Epitope / Immunogen

          Conjugate

          DyLight™ 488 DyLight™ 488 DyLight™ 488
          • Unconjugated
          • Biotin 
          • HRP
          • Request custom conjugation

          Excitation/Emission Max

          492/519 nm View spectra spectra

          Form

          Liquid

          Concentration

          0.71 mg/mL

          Amount

          71 µg

          Purification

          Protein G

          Storage buffer

          50mM sodium borate

          Contains

          0.05% sodium azide

          Storage conditions

          4°C, store in dark

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_11157208

          Product Specific Information

          Suggested positive control: Cos7 CoCl2-treated nuclear extract.

          Target Information

          HIF1-alpha (HIF1A) is a subunit of HIF1, which is a transcription factor found in mammalian cells cultured under reduced oxygen tension. HIF-1 is a heterodimer consisting of an alpha and beta subunit, both belonging to the basic-helix-loop-helix Per-aryl hydrocarbon receptor nuclear translocator-Sim (PAS) family of transcription factors. HIF1 functions as a transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions, HIF-1 activates the transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, HILPDA, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. HIF1-alpha regulates hypoxia-mediated apoptosis, cell proliferation and tumor angiogenesis. Hypoxia which induces p53 protein accumulation, directly interacts with HIF1-alpha and reduces hypoxia-induced expression of HIF1-alpha by promoting MDM2-mediated ubiquitination and proteasomal degradation under hypoxic conditions. Recent studies suggest that induction of NOX4 by HIF1-alpha contributes to maintain ROS levels after hypoxia and hypoxia-induced proliferation. In humans, it is located on the q arm of chromosome 14. The C-terminal of HIF1A binds to p300. p300/CBP-HIF complexes participate in the induction of hypoxia-responsive genes, including VEGF. Hypoxia contributes significantly to the pathophysiology of major categories of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease and chronic obstructive pulmonary disease.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          How to use the Panel Builder

          Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

          Bioinformatics

          Protein Aliases: ARNT interacting protein; ARNT-interacting protein; ARNT2; Basic-helix-loop-helix-PAS protein MOP1; bHLHe78; Class E basic helix-loop-helix protein 78; hif 1; hif 1a; HIF-1 alpha; HIF-1-alpha; HIF-1alpha 786; HIF1 alpha; HIF1-alpha; hypoxia inducible factor 1 alpha subunit; hypoxia inducible factor 1, alpha subunit; hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor); hypoxia-inducible factor 1 alpha; hypoxia-inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor); Hypoxia-inducible factor 1-alpha; hypoxia-inducible factor1alpha; Member of PAS protein 1; member of PAS superfamily 1; PAS domain-containing protein 8; transcription factor; transcription factor; hypoxia-inducible factor 1a; unnamed protein product

          View more View less

          Gene Aliases: AA959795; BHLHE78; HIF-1-alpha; HIF-1A; HIF-1alpha; HIF1; HIF1-ALPHA; HIF1A; HIF1alpha; MOP1; PASD8

          View more View less

          UniProt ID: (Mouse) Q61221, (Rat) O35800

          View more View less

          Entrez Gene ID: (Bovine) 281814, (Human) 3091, (Dog) 480348, (Mouse) 15251, (Rat) 29560

          View more View less

          Function(s)
          transcription factor activity, sequence-specific DNA binding histone acetyltransferase binding sequence-specific DNA binding protein heterodimerization activity RNA polymerase II regulatory region sequence-specific DNA binding RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding core promoter proximal region sequence-specific DNA binding bacterial-type RNA polymerase transcriptional activator activity, sequence-specific DNA binding bacterial-type RNA polymerase transcriptional repressor activity, sequence-specific DNA binding transcription corepressor binding transcription coactivator binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding p53 binding DNA binding protein binding ligand-dependent nuclear receptor binding enzyme binding protein kinase binding protein domain specific binding ubiquitin protein ligase binding histone deacetylase binding protein dimerization activity Hsp90 protein binding RNA polymerase II sequence-specific DNA binding transcription factor binding E-box binding transcription regulator activator activity transcription factor activity, transcription factor binding transcription factor activity, RNA polymerase II transcription factor binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding signal transducer activity transcription factor binding protein complex binding nuclear hormone receptor binding double-stranded DNA binding basic helix-loop-helix transcription factor
          Process(es)
          response to hypoxia positive regulation of vascular endothelial growth factor production axonal transport of mitochondrion positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter positive regulation of transcription from RNA polymerase II promoter in response to hypoxia cellular response to hypoxia response to reactive oxygen species cellular glucose homeostasis epithelial to mesenchymal transition positive regulation of endothelial cell proliferation connective tissue replacement involved in inflammatory response wound healing regulation of glycolytic process regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter signal transduction response to iron ion regulation of gene expression vascular endothelial growth factor production positive regulation of gene expression negative regulation of gene expression positive regulation of epithelial cell migration negative regulation of ossification positive regulation of vascular endothelial growth factor receptor signaling pathway oxygen homeostasis positive regulation of chemokine production regulation of transforming growth factor beta2 production collagen metabolic process cellular response to oxidative stress positive regulation of blood vessel endothelial cell migration positive regulation of erythrocyte differentiation positive regulation of angiogenesis positive regulation of glycolytic process positive regulation of hormone biosynthetic process elastin metabolic process positive regulation of chemokine-mediated signaling pathway cellular response to interleukin-1 cellular response to virus positive regulation of cytokine production involved in inflammatory response negative regulation of pri-miRNA transcription from RNA polymerase II promoter positive regulation of pri-miRNA transcription from RNA polymerase II promoter negative regulation of oxidative stress-induced neuron intrinsic apoptotic signaling pathway negative regulation of reactive oxygen species biosynthetic process regulation of protein neddylation angiogenesis blood vessel development neural crest cell migration embryonic placenta development B-1 B cell homeostasis vasculature development heart looping positive regulation of neuroblast proliferation outflow tract morphogenesis cardiac ventricle morphogenesis lactate metabolic process transcription, DNA-templated transcription from RNA polymerase II promoter cellular iron ion homeostasis lactation positive regulation of cell proliferation visual learning positive regulation of autophagy positive regulation of receptor biosynthetic process response to muscle activity neural fold elevation formation cerebral cortex development cell differentiation negative regulation of bone mineralization negative regulation of TOR signaling negative regulation of transcription elongation from RNA polymerase II promoter embryonic hemopoiesis positive regulation of insulin secretion involved in cellular response to glucose stimulus regulation of cell proliferation hemoglobin biosynthetic process glucose homeostasis positive regulation of apoptotic process negative regulation of apoptotic process negative regulation of neuron apoptotic process regulation of transcription from RNA polymerase II promoter in response to oxidative stress negative regulation of vasoconstriction negative regulation of growth muscle cell cellular homeostasis blood vessel morphogenesis digestive tract morphogenesis positive regulation of smooth muscle cell proliferation regulation of catalytic activity cartilage development intestinal epithelial cell maturation response to fungicide epithelial cell differentiation involved in mammary gland alveolus development retina vasculature development in camera-type eye regulation of thymocyte apoptotic process negative regulation of thymocyte apoptotic process dopaminergic neuron differentiation hypoxia-inducible factor-1alpha signaling pathway positive regulation of mitophagy regulation of aerobic respiration negative regulation of reactive oxygen species metabolic process negative regulation of mesenchymal cell apoptotic process response to mechanical stimulus response to salt stress response to glucose response to X-ray response to auditory stimulus response to purine-containing compound response to activity response to cobalt ion response to estradiol cellular response to insulin stimulus cellular response to drug response to drug response to alkaloid response to estrogen positive regulation of cell size response to glucocorticoid maternal process involved in female pregnancy cellular response to hydrogen peroxide cellular response to lipopolysaccharide cellular response to carbon monoxide cellular response to nitrite cellular response to electrical stimulus cellular response to mechanical stimulus cellular response to cobalt ion cellular response to glucose stimulus cellular response to lipid cellular response to organic cyclic compound cellular response to light stimulus response to anesthetic cellular response to toxic substance regulation of cellular response to hypoxia cellular response to cyanide
          It has to be done as per old AB suggested Products section.

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