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Invitrogen
Survivin Monoclonal Antibody (1H5)
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FIGURE: 1 / 11
Survivin Antibody (MA5-17035) in ICC/IF
Immunofluorescence analysis of Survivin was performed using 70 % confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Survivin Monoclonal Antibody (1H5) (MA5-17035) at 1:250 dilution in 0.1% BSA and incubated for overnight at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (A28175) at a d... View More
Product Details
MA5-17035
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
C. elegans
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
1H5
Immunogen
Purified recombinant fragment of human BIRC5 expressed in E. Coli.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein G
Storage buffer
PBS
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
MA5-17035 targets BIRC5 in indirect ELISA, FACS, ICC, IHC, IF and WB applications and shows reactivity with Human samples.
The MA5-17035 immunogen is purified recombinant fragment of human BIRC5 expressed in E. Coli.
MA5-17035 detects BIRC5 which has a predicted molecular weight of approximately 16kDa.
Target Information
Survivin (IAP4) is the smallest member of the Inhibitors of Apoptosis Protein (IAP) gene family. The IAPs are involved in multiple cell functions such as cell signaling, cell division, metabolism, and exhibits differential expression in nearly all human cancers, but not in most normal tissues. IAP family members usually contain multiple baculovirus IAP repeat (BIR) domains, but the Survivin gene encodes proteins with only a single BIR domain. The encoded proteins also lack a C-terminus RING finger domain. Gene expression is high during fetal development and in most tumors yet low in adult tissues. Antisense transcripts are involved in the regulation of survivin's gene expression. Survivin is expressed in the G2/M phase of the cell cycle in a cycle-regulated manner. At the beginning of mitosis, survivin associates with microtubules of the mitotic spindle in a specific and saturable reaction that is regulated by microtubule dynamics. Disruption of survivin-microtubule interactions results in loss of survivin's anti-apoptosis function and increased caspase-3 activity, a mechanism involved in cell death, during mitosis. Survivin may counteract a default induction of apoptosis in G2/M phase. Survivin is also abundantly expressed in brain tissues (astrocytes and some neurons) of adult rats following traumatic brain injury. Survivin has been found co-expressed with NeuN (mature neuronal marker) and PCNA (a cell cycle protein). Survivin might affect regulation of neural cell proliferative responses after brain injury. At least four transcript variants encoding distinct isoforms have been found for this gene, but the full-length natures of only three of them have been determined. The overexpression of survivin in cancer may overcome this apoptotic checkpoint and favor aberrant progression of transformed cells through mitosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Apoptosis inhibitor 4; Apoptosis inhibitor survivin; Baculoviral IAP repeat-containing protein 5; BIRC 5; EPR 1; survivin variant 3 alpha; SVV
Gene Aliases: API4; BIRC5; EPR-1; IAP4
UniProt ID: (Human) O15392
Entrez Gene ID: (Human) 332
transcription, DNA-templated
protein phosphorylation
apoptotic process
sister chromatid cohesion
positive regulation of cell proliferation
regulation of signal transduction
protein ubiquitination
protein sumoylation
protein complex localization
regulation of apoptotic process
negative regulation of apoptotic process
negative regulation of transcription, DNA-templated
cell division
mitotic spindle assembly
inhibition of cysteine-type endopeptidase activity involved in apoptotic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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