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Invitrogen
Hexanoyl-Lysine adduct Monoclonal Antibody (5E8)
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Hexanoyl-Lysine adduct Antibody (MA5-27555) in ICC/IF
Immunofluorescent analysis of Hexanoyl-Lysine adduct in human embryonic kidney cells (HEK293). Sample was fixed with 5% formaldehyde (5 min), incubated with Hexanoyl-Lysine adduct monoclonal antibody (Product # MA5-27555) using a dilution of 1:50 (30-60 min at RT), and followed by Goat Anti-Mouse Alexa Fluor 488, Phalloidin Alexa Fluor 633 and DAPI secondary antibody at a dilution of 1:1500, 1:250 and 1:50,... View More
产品信息
MA5-27555
应用
建议稀释比
已发表文章
产品规格
种属反应
Chemical
宿主/亚型
Mouse
/ IgG1
分类
Monoclonal
类型
Antibody
克隆号
5E8
抗原
Synthetic Hexanoyl modified Keyhole Limpet Hemocyanin (KLH)
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein G
保存液
PBS, pH 7.4, with 50% glycerol
内含物
0.09% sodium azide
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
A 1:1,000 dilution of MA5-27555 was sufficient for detection of Hexanoyl Lysine adduct in 0.5 µg of Hexanoyl Lysine conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody.|Specific for Hexanoyl-Lysine adduct (HEL) modified peptides and proteins. Does not detect free Hexanoyl-Lysine. Does not cross-react with Acrolein, Crotonaldehyde, 4-Hydroxy-2-hexenal, 4-Hydroxy nonenal, Malondialdehyde, or Methylglyoxal modified proteins.
靶标信息
Hexanoyl-lysine (HEL) is a novel lipid peroxidation biomarker which is derived from the oxidation of omega-6 unsaturated fatty acid. HEL may be used as a biomarker in omega-6 PUFA (polyunsaturated fatty acid) oxidation in living organisms.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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